Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse
<b>Background:</b> The increase in the collagen I (COL I)/COL III ratio enhances vessel wall stiffness and renders vessels less resistant to blood flow and pressure changes. Activated microglia enhance inflammation-induced fibrosis. <b>Hypotheses:</b> The COL I/COL III ratio...
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MDPI AG
2024-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/13/1/92 |
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| author | Zahra Shabani Joana Schuerger Xiaonan Zhu Chaoliang Tang Li Ma Alka Yadav Rich Liang Kelly Press Shantel Weinsheimer Annika Schmidt Calvin Wang Abinav Sekhar Jeffrey Nelson Helen Kim Hua Su |
| author_facet | Zahra Shabani Joana Schuerger Xiaonan Zhu Chaoliang Tang Li Ma Alka Yadav Rich Liang Kelly Press Shantel Weinsheimer Annika Schmidt Calvin Wang Abinav Sekhar Jeffrey Nelson Helen Kim Hua Su |
| author_sort | Zahra Shabani |
| collection | DOAJ |
| description | <b>Background:</b> The increase in the collagen I (COL I)/COL III ratio enhances vessel wall stiffness and renders vessels less resistant to blood flow and pressure changes. Activated microglia enhance inflammation-induced fibrosis. <b>Hypotheses:</b> The COL I/COL III ratio in human and mouse brain arteriovenous malformations (bAVMs) is associated with bAVM hemorrhage, and the depletion of microglia decreases the COL I/COL III ratio and hemorrhage. <b>Method:</b> COL I, COL III, and hemorrhages were analyzed in 12 human bAVMs and 6 control brains, and mouse bAVMs induced in three mouse lines with activin receptor-like kinase 1 (<i>n</i> = 7) or endoglin (<i>n</i> = 7) deleted in the endothelial cells or brain focally (<i>n</i> = 5). The controls for the mouse study were no-gene-deleted litter mates. Mouse bAVMs were used to test the relationships between the Col I/Col III ratio and hemorrhage and whether the transient depletion of microglia reduces the Col I/Col III ratio and hemorrhage. <b>Results:</b> The COL I/COL III ratio was higher in the human and mouse bAVMs than in controls. The microhemorrhage in mouse bAVMs was positively correlated with the Col I/Col III ratio. Transient depletion of microglia reduced the Col I/Col III ratio and microhemorrhage. <b>Conclusions:</b> The COL I/COL III ratio in the bAVMs was associated with bAVM hemorrhage. The depletion of microglia reduced the bAVM Col I/Col III ratio and hemorrhage. |
| first_indexed | 2024-03-08T15:09:56Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-08T15:09:56Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f8a5d4898637431194463104c0117eea2024-01-10T14:53:30ZengMDPI AGCells2073-44092024-01-011319210.3390/cells13010092Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and MouseZahra Shabani0Joana Schuerger1Xiaonan Zhu2Chaoliang Tang3Li Ma4Alka Yadav5Rich Liang6Kelly Press7Shantel Weinsheimer8Annika Schmidt9Calvin Wang10Abinav Sekhar11Jeffrey Nelson12Helen Kim13Hua Su14Center for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USACenter for Cerebrovascular Research, University of California, San Francisco, CA 94143, USA<b>Background:</b> The increase in the collagen I (COL I)/COL III ratio enhances vessel wall stiffness and renders vessels less resistant to blood flow and pressure changes. Activated microglia enhance inflammation-induced fibrosis. <b>Hypotheses:</b> The COL I/COL III ratio in human and mouse brain arteriovenous malformations (bAVMs) is associated with bAVM hemorrhage, and the depletion of microglia decreases the COL I/COL III ratio and hemorrhage. <b>Method:</b> COL I, COL III, and hemorrhages were analyzed in 12 human bAVMs and 6 control brains, and mouse bAVMs induced in three mouse lines with activin receptor-like kinase 1 (<i>n</i> = 7) or endoglin (<i>n</i> = 7) deleted in the endothelial cells or brain focally (<i>n</i> = 5). The controls for the mouse study were no-gene-deleted litter mates. Mouse bAVMs were used to test the relationships between the Col I/Col III ratio and hemorrhage and whether the transient depletion of microglia reduces the Col I/Col III ratio and hemorrhage. <b>Results:</b> The COL I/COL III ratio was higher in the human and mouse bAVMs than in controls. The microhemorrhage in mouse bAVMs was positively correlated with the Col I/Col III ratio. Transient depletion of microglia reduced the Col I/Col III ratio and microhemorrhage. <b>Conclusions:</b> The COL I/COL III ratio in the bAVMs was associated with bAVM hemorrhage. The depletion of microglia reduced the bAVM Col I/Col III ratio and hemorrhage.https://www.mdpi.com/2073-4409/13/1/92brain arteriovenous malformationcollagen Icollagen IIImicrohemorrhagedepletion of microgliagluycoprotein nonmetastatic B |
| spellingShingle | Zahra Shabani Joana Schuerger Xiaonan Zhu Chaoliang Tang Li Ma Alka Yadav Rich Liang Kelly Press Shantel Weinsheimer Annika Schmidt Calvin Wang Abinav Sekhar Jeffrey Nelson Helen Kim Hua Su Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse Cells brain arteriovenous malformation collagen I collagen III microhemorrhage depletion of microglia gluycoprotein nonmetastatic B |
| title | Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse |
| title_full | Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse |
| title_fullStr | Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse |
| title_full_unstemmed | Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse |
| title_short | Increased Collagen I/Collagen III Ratio Is Associated with Hemorrhage in Brain Arteriovenous Malformations in Human and Mouse |
| title_sort | increased collagen i collagen iii ratio is associated with hemorrhage in brain arteriovenous malformations in human and mouse |
| topic | brain arteriovenous malformation collagen I collagen III microhemorrhage depletion of microglia gluycoprotein nonmetastatic B |
| url | https://www.mdpi.com/2073-4409/13/1/92 |
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