Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17
PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress. However, there has been very little research on the function of this family gene in tomatoes, which limits its application in germplasm resource impr...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923002654 |
| _version_ | 1797356374243409920 |
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| author | Min Xu Zhao Gao Dalong Li Chen Zhang Yuqi Zhang Qian He Yingbin Qi He Zhang Jingbin Jiang Xiangyang Xu Tingting Zhao |
| author_facet | Min Xu Zhao Gao Dalong Li Chen Zhang Yuqi Zhang Qian He Yingbin Qi He Zhang Jingbin Jiang Xiangyang Xu Tingting Zhao |
| author_sort | Min Xu |
| collection | DOAJ |
| description | PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress. However, there has been very little research on the function of this family gene in tomatoes, which limits its application in germplasm resource improvement. Therefore, the PLATZ gene family was identified and analyzed in tomato, and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function. In this study, the PLATZ family members of tomato were identified in the whole genome, and 19 SlPLATZ genes were obtained. Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis. SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources. SlPLATZ17 was selected for functional verification by experiment-based analysis. The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants. Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long, thin petals, adjacent petals were not connected at the base, and the stamen circumference was smaller. This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening. |
| first_indexed | 2024-03-08T14:26:41Z |
| format | Article |
| id | doaj.art-f8a7a56e915847b99b01cf44493e4b3e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-08T14:26:41Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-f8a7a56e915847b99b01cf44493e4b3e2024-01-13T04:44:04ZengElsevierJournal of Integrative Agriculture2095-31192024-01-01231141154Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17Min Xu0Zhao Gao1Dalong Li2Chen Zhang3Yuqi Zhang4Qian He5Yingbin Qi6He Zhang7Jingbin Jiang8Xiangyang Xu9Tingting Zhao10College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, China; Correspondence Xiangyang XuCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, Harbin 150030, China; Correspondence Tingting ZhaoPLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress. However, there has been very little research on the function of this family gene in tomatoes, which limits its application in germplasm resource improvement. Therefore, the PLATZ gene family was identified and analyzed in tomato, and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function. In this study, the PLATZ family members of tomato were identified in the whole genome, and 19 SlPLATZ genes were obtained. Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis. SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources. SlPLATZ17 was selected for functional verification by experiment-based analysis. The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants. Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long, thin petals, adjacent petals were not connected at the base, and the stamen circumference was smaller. This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.http://www.sciencedirect.com/science/article/pii/S2095311923002654tomatoPLATZ familyabiotic stressesplant development |
| spellingShingle | Min Xu Zhao Gao Dalong Li Chen Zhang Yuqi Zhang Qian He Yingbin Qi He Zhang Jingbin Jiang Xiangyang Xu Tingting Zhao Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 Journal of Integrative Agriculture tomato PLATZ family abiotic stresses plant development |
| title | Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 |
| title_full | Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 |
| title_fullStr | Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 |
| title_full_unstemmed | Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 |
| title_short | Functional prediction of tomato PLATZ family members and functional verification of SlPLATZ17 |
| title_sort | functional prediction of tomato platz family members and functional verification of slplatz17 |
| topic | tomato PLATZ family abiotic stresses plant development |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923002654 |
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