Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
Over the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1345 |
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| author | Delia Michiu Maria-Ioana Socaciu Melinda Fogarasi Anamaria Mirela Jimborean Floricuţa Ranga Vlad Mureşan Cristina Anamaria Semeniuc |
| author_facet | Delia Michiu Maria-Ioana Socaciu Melinda Fogarasi Anamaria Mirela Jimborean Floricuţa Ranga Vlad Mureşan Cristina Anamaria Semeniuc |
| author_sort | Delia Michiu |
| collection | DOAJ |
| description | Over the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical method to assess the total phenolic content (TPC) in essential oils using Folin–Ciocalteu’s phenol reagent and quantifies the individual phenolic compounds by liquid chromatography. Thus, the research design and methodology included: (1) extraction of essential oil from dried thyme leaves by hydrodistillation; (2) spectrophotometric measurement of TPC by Folin–Ciocalteu method; and (3) identification and quantification of individual phenolic compounds by high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). Results revealed a TPC of 22.62 ± 0.482 mg GAE/100 µL and a polyphenolic profile characterized by phenolic acids (52.1%), flavonoids (16.1%), and other polyphenols (31.8%). Thymol, salvianolic acid A, and rosmarinic acid were the major compounds of thyme essential oil. The proposed analytical procedure has an acceptable level of repeatability, reproducibility, linearity, LOD (limit of detection), and LOQ (limit of quantification). |
| first_indexed | 2024-03-09T21:21:20Z |
| format | Article |
| id | doaj.art-f8a8069e81d84fb68a160c78cea7e306 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:21:20Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f8a8069e81d84fb68a160c78cea7e3062023-11-23T21:22:31ZengMDPI AGMolecules1420-30492022-02-01274134510.3390/molecules27041345Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential OilsDelia Michiu0Maria-Ioana Socaciu1Melinda Fogarasi2Anamaria Mirela Jimborean3Floricuţa Ranga4Vlad Mureşan5Cristina Anamaria Semeniuc6Department of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Science, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaDepartment of Food Engineering, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăştur Str., 400372 Cluj-Napoca, RomaniaOver the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical method to assess the total phenolic content (TPC) in essential oils using Folin–Ciocalteu’s phenol reagent and quantifies the individual phenolic compounds by liquid chromatography. Thus, the research design and methodology included: (1) extraction of essential oil from dried thyme leaves by hydrodistillation; (2) spectrophotometric measurement of TPC by Folin–Ciocalteu method; and (3) identification and quantification of individual phenolic compounds by high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). Results revealed a TPC of 22.62 ± 0.482 mg GAE/100 µL and a polyphenolic profile characterized by phenolic acids (52.1%), flavonoids (16.1%), and other polyphenols (31.8%). Thymol, salvianolic acid A, and rosmarinic acid were the major compounds of thyme essential oil. The proposed analytical procedure has an acceptable level of repeatability, reproducibility, linearity, LOD (limit of detection), and LOQ (limit of quantification).https://www.mdpi.com/1420-3049/27/4/1345total phenolic contentessential oilsFolin–Ciocalteu methodUV-Vis spectrophotometrypolyphenolic compoundsHPLC-DAD-ESI-MS analysis |
| spellingShingle | Delia Michiu Maria-Ioana Socaciu Melinda Fogarasi Anamaria Mirela Jimborean Floricuţa Ranga Vlad Mureşan Cristina Anamaria Semeniuc Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils Molecules total phenolic content essential oils Folin–Ciocalteu method UV-Vis spectrophotometry polyphenolic compounds HPLC-DAD-ESI-MS analysis |
| title | Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils |
| title_full | Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils |
| title_fullStr | Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils |
| title_full_unstemmed | Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils |
| title_short | Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils |
| title_sort | implementation of an analytical method for spectrophotometric evaluation of total phenolic content in essential oils |
| topic | total phenolic content essential oils Folin–Ciocalteu method UV-Vis spectrophotometry polyphenolic compounds HPLC-DAD-ESI-MS analysis |
| url | https://www.mdpi.com/1420-3049/27/4/1345 |
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