The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress
SMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among the growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function of SAUR family member AtSA...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.625493/full |
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| author | Yanjun He Yue Liu Mengzhuo Li Anthony Tumbeh Lamin-Samu Dandan Yang Xiaolin Yu Muhammad Izhar Ibadullah Jan Muhammad Ali Gang Lu Gang Lu |
| author_facet | Yanjun He Yue Liu Mengzhuo Li Anthony Tumbeh Lamin-Samu Dandan Yang Xiaolin Yu Muhammad Izhar Ibadullah Jan Muhammad Ali Gang Lu Gang Lu |
| author_sort | Yanjun He |
| collection | DOAJ |
| description | SMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among the growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function of SAUR family member AtSAUR32 has the potential to diminish the negative impact of drought stress, but the exact function and mode of action remain unclear in Arabidopsis. In the current study, AtSAUR32 gene was cloned and functionally analyzed. AtSAUR32 localized to the plasma membrane and nucleus was dominantly expressed in roots and highly induced by abscisic acid and drought treatment at certain time points. The stomatal closure and seed germination of saur32 were less sensitive to ABA relative to AtSAUR32-overexpressed line (OE32-5) and wild type (WT). Moreover, the saur32 mutant under drought stress showed increased ion leakage while quantum yield of photosystem II (ΦPSII) and endogenous ABA accumulation were reduced, along with the expression pattern of ABA/stress-responsive genes compared with WT and the OE32-5 transgenic line. Additionally, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that AtSAUR32 interacted with clade-A PP2C proteins (AtHAI1 and AtAIP1) to regulate ABA sensitivity in Arabidopsis. Taken together, these results indicate that AtSAUR32 plays an important role in drought stress adaptation via mediating ABA signal transduction. |
| first_indexed | 2024-12-13T23:36:36Z |
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| id | doaj.art-f8b022bc8d934d948de5b8e5eceec2fa |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T23:36:36Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f8b022bc8d934d948de5b8e5eceec2fa2022-12-21T23:27:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-03-011210.3389/fpls.2021.625493625493The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought StressYanjun He0Yue Liu1Mengzhuo Li2Anthony Tumbeh Lamin-Samu3Dandan Yang4Xiaolin Yu5Muhammad Izhar6Ibadullah Jan7Muhammad Ali8Gang Lu9Gang Lu10Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Agronomy, Northwest A&F University, Yangling, ChinaDepartment of Agriculture, University of Swabi, Swabi, PakistanDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaKey Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Zhejiang University, Hangzhou, ChinaSMALL AUXIN UP-REGULATED RNAs (SAURs) are recognized as auxin-responsive genes involved in the regulation of abiotic stress adaptive growth. Among the growth-limiting factors, water-deficit condition significantly affects plant growth and development. The putative function of SAUR family member AtSAUR32 has the potential to diminish the negative impact of drought stress, but the exact function and mode of action remain unclear in Arabidopsis. In the current study, AtSAUR32 gene was cloned and functionally analyzed. AtSAUR32 localized to the plasma membrane and nucleus was dominantly expressed in roots and highly induced by abscisic acid and drought treatment at certain time points. The stomatal closure and seed germination of saur32 were less sensitive to ABA relative to AtSAUR32-overexpressed line (OE32-5) and wild type (WT). Moreover, the saur32 mutant under drought stress showed increased ion leakage while quantum yield of photosystem II (ΦPSII) and endogenous ABA accumulation were reduced, along with the expression pattern of ABA/stress-responsive genes compared with WT and the OE32-5 transgenic line. Additionally, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that AtSAUR32 interacted with clade-A PP2C proteins (AtHAI1 and AtAIP1) to regulate ABA sensitivity in Arabidopsis. Taken together, these results indicate that AtSAUR32 plays an important role in drought stress adaptation via mediating ABA signal transduction.https://www.frontiersin.org/articles/10.3389/fpls.2021.625493/fullABAArabidopsisAtSAUR32droughtPP2C |
| spellingShingle | Yanjun He Yue Liu Mengzhuo Li Anthony Tumbeh Lamin-Samu Dandan Yang Xiaolin Yu Muhammad Izhar Ibadullah Jan Muhammad Ali Gang Lu Gang Lu The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress Frontiers in Plant Science ABA Arabidopsis AtSAUR32 drought PP2C |
| title | The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress |
| title_full | The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress |
| title_fullStr | The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress |
| title_full_unstemmed | The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress |
| title_short | The Arabidopsis SMALL AUXIN UP RNA32 Protein Regulates ABA-Mediated Responses to Drought Stress |
| title_sort | arabidopsis small auxin up rna32 protein regulates aba mediated responses to drought stress |
| topic | ABA Arabidopsis AtSAUR32 drought PP2C |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.625493/full |
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