The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils
Hypermucoviscosity phenotypic <i>Klebsiella pneumoniae</i> (HV-<i>Kp</i>) serotype K1 is the predominant pathogen of a pyogenic liver abscess, an emerging infectious disease that often complicates septic metastatic syndrome in diabetic patients with poor sugar control. HV-<...
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MDPI AG
2020-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1009 |
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| author | Chen-Hsiang Lee Seng-Kee Chuah Chia-Chi Chang Fang-Ju Chen |
| author_facet | Chen-Hsiang Lee Seng-Kee Chuah Chia-Chi Chang Fang-Ju Chen |
| author_sort | Chen-Hsiang Lee |
| collection | DOAJ |
| description | Hypermucoviscosity phenotypic <i>Klebsiella pneumoniae</i> (HV-<i>Kp</i>) serotype K1 is the predominant pathogen of a pyogenic liver abscess, an emerging infectious disease that often complicates septic metastatic syndrome in diabetic patients with poor sugar control. HV-<i>Kp</i>isolates were more resistant to neutrophil phagocytosis than non-HV-<i>Kp</i>isolates because of different pathogen-associated molecular patterns. The protein expression of HV-<i>Kp</i> after interaction with neutrophils is unclear. We studied KP-M1 (HV phenotype; serotype K<sub>1</sub>), DT-X (an acapsularmutant strain of KP-M1), and <i>E. coli</i> (ATCC 25922) with the model of <i>Kp-</i>infected neutrophils, using a comparative proteomic approach. One the identified protein, namely fructose-1, 6-bisphosphate aldolase (FBA), was found to be distributed in the KP-M1 after infecting neutrophils. Cell fractionation experiments showed that FBA is localized both to the cytoplasm and the outer membrane. Flow cytometry demonstrated that outer membrane-localized FBA was surface-accessible to FBA-specific antibody. The <i>fba</i> gene expression was enhanced in high glucose concentrations, which leads to increasing bacterial resistance to neutrophils phagocytosis and killing. The KP-M1 after FBA inhibitors and FBA-specific antibody treatment showed a significant reduction in bacterial resistance to neutrophils phagocytosis and killing, respectively, compared to KP-M1 without treatment. FBA is a highly conserved surface-exposed protein that is required for optimal interaction of HV-<i>Kp</i> to neutrophils. |
| first_indexed | 2024-03-10T14:25:48Z |
| format | Article |
| id | doaj.art-f8b6f3741bdd417cb64669baf0c4294d |
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| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T14:25:48Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-f8b6f3741bdd417cb64669baf0c4294d2023-11-20T23:03:12ZengMDPI AGPathogens2076-08172020-11-01912100910.3390/pathogens9121009The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with NeutrophilsChen-Hsiang Lee0Seng-Kee Chuah1Chia-Chi Chang2Fang-Ju Chen3Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83304, TaiwanDivision of Gastroenterology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83304, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83304, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83304, TaiwanHypermucoviscosity phenotypic <i>Klebsiella pneumoniae</i> (HV-<i>Kp</i>) serotype K1 is the predominant pathogen of a pyogenic liver abscess, an emerging infectious disease that often complicates septic metastatic syndrome in diabetic patients with poor sugar control. HV-<i>Kp</i>isolates were more resistant to neutrophil phagocytosis than non-HV-<i>Kp</i>isolates because of different pathogen-associated molecular patterns. The protein expression of HV-<i>Kp</i> after interaction with neutrophils is unclear. We studied KP-M1 (HV phenotype; serotype K<sub>1</sub>), DT-X (an acapsularmutant strain of KP-M1), and <i>E. coli</i> (ATCC 25922) with the model of <i>Kp-</i>infected neutrophils, using a comparative proteomic approach. One the identified protein, namely fructose-1, 6-bisphosphate aldolase (FBA), was found to be distributed in the KP-M1 after infecting neutrophils. Cell fractionation experiments showed that FBA is localized both to the cytoplasm and the outer membrane. Flow cytometry demonstrated that outer membrane-localized FBA was surface-accessible to FBA-specific antibody. The <i>fba</i> gene expression was enhanced in high glucose concentrations, which leads to increasing bacterial resistance to neutrophils phagocytosis and killing. The KP-M1 after FBA inhibitors and FBA-specific antibody treatment showed a significant reduction in bacterial resistance to neutrophils phagocytosis and killing, respectively, compared to KP-M1 without treatment. FBA is a highly conserved surface-exposed protein that is required for optimal interaction of HV-<i>Kp</i> to neutrophils.https://www.mdpi.com/2076-0817/9/12/1009innate immunityvirulencepathogenesismetastatic infectionprotein expression |
| spellingShingle | Chen-Hsiang Lee Seng-Kee Chuah Chia-Chi Chang Fang-Ju Chen The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils Pathogens innate immunity virulence pathogenesis metastatic infection protein expression |
| title | The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils |
| title_full | The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils |
| title_fullStr | The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils |
| title_full_unstemmed | The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils |
| title_short | The Surface Protein Fructose-1, 6 Bisphosphate Aldolase of <i>Klebsiella pneumoniae</i> Serotype K1: Role of Interaction with Neutrophils |
| title_sort | surface protein fructose 1 6 bisphosphate aldolase of i klebsiella pneumoniae i serotype k1 role of interaction with neutrophils |
| topic | innate immunity virulence pathogenesis metastatic infection protein expression |
| url | https://www.mdpi.com/2076-0817/9/12/1009 |
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