Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results?
Purpose: molecular testing is often indicated for recently transfused patients. However, there are no guidelines regarding the potential interference from donor DNA or whether it is necessary to wait for a period of time post-transfusion prior to genetic testing. While the majority of patients are t...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/10/4/268 |
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| author | Maggie A. DiGuardo Sarah J. Kester Victor J. Mahaffey Scott A. Hammel Katelyn K. Heaser Christopher D. Hofich Craig D. Tauscher Sarah E. Kerr Jennifer L. Oliveira Eapen K. Jacob Ann M. Moyer |
| author_facet | Maggie A. DiGuardo Sarah J. Kester Victor J. Mahaffey Scott A. Hammel Katelyn K. Heaser Christopher D. Hofich Craig D. Tauscher Sarah E. Kerr Jennifer L. Oliveira Eapen K. Jacob Ann M. Moyer |
| author_sort | Maggie A. DiGuardo |
| collection | DOAJ |
| description | Purpose: molecular testing is often indicated for recently transfused patients. However, there are no guidelines regarding the potential interference from donor DNA or whether it is necessary to wait for a period of time post-transfusion prior to genetic testing. While the majority of patients are transfused in the non-trauma setting using leukoreduced (LR) red blood cell products, the degree of leukoreduction varies among centers and is not universally practiced. Methods: whole blood units collected from anonymous donors were used in an in vitro transfusion model. One unit was split: half being leukoreduced simulating a leukopenic recipient and half left untreated. Donors were simulated by leukoreduced, partially leukoreduced (PLR), or non-leukoreduced units, transfused in 2, 5, or 16 unit equivalents. DNA from the combinations were subjected to short tandem repeat (STR) analysis for chimerism detection. Results: donor DNA was not detectable in any of the LR combinations, but detected in the PLR combinations, ranging from 0.1 to 1.5% donor DNA in the immunocompetent recipient and 6.3–27.8% in the leukopenic recipient. Non-LR donor DNA was also detected (13–95%). Conclusion: donor-derived DNA from leukoreduced blood products is unlikely to interfere with the interpretation of germline genetic testing in immunocompetent recipients but may interfere in immunocompromised recipients. |
| first_indexed | 2024-03-10T14:14:09Z |
| format | Article |
| id | doaj.art-f8bcbe3e1e574b73a158d32d52e460a6 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-10T14:14:09Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-f8bcbe3e1e574b73a158d32d52e460a62023-11-20T23:58:02ZengMDPI AGJournal of Personalized Medicine2075-44262020-12-0110426810.3390/jpm10040268Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results?Maggie A. DiGuardo0Sarah J. Kester1Victor J. Mahaffey2Scott A. Hammel3Katelyn K. Heaser4Christopher D. Hofich5Craig D. Tauscher6Sarah E. Kerr7Jennifer L. Oliveira8Eapen K. Jacob9Ann M. Moyer10Division of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Hematopathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USAHospital Pathology Associates, Minneapolis, MN 55407, USADivision of Hematopathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Transfusion Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USADivision of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USAPurpose: molecular testing is often indicated for recently transfused patients. However, there are no guidelines regarding the potential interference from donor DNA or whether it is necessary to wait for a period of time post-transfusion prior to genetic testing. While the majority of patients are transfused in the non-trauma setting using leukoreduced (LR) red blood cell products, the degree of leukoreduction varies among centers and is not universally practiced. Methods: whole blood units collected from anonymous donors were used in an in vitro transfusion model. One unit was split: half being leukoreduced simulating a leukopenic recipient and half left untreated. Donors were simulated by leukoreduced, partially leukoreduced (PLR), or non-leukoreduced units, transfused in 2, 5, or 16 unit equivalents. DNA from the combinations were subjected to short tandem repeat (STR) analysis for chimerism detection. Results: donor DNA was not detectable in any of the LR combinations, but detected in the PLR combinations, ranging from 0.1 to 1.5% donor DNA in the immunocompetent recipient and 6.3–27.8% in the leukopenic recipient. Non-LR donor DNA was also detected (13–95%). Conclusion: donor-derived DNA from leukoreduced blood products is unlikely to interfere with the interpretation of germline genetic testing in immunocompetent recipients but may interfere in immunocompromised recipients.https://www.mdpi.com/2075-4426/10/4/268transfusiongenetic testingleukoreductiontransfusion-associated microchimerisminterference |
| spellingShingle | Maggie A. DiGuardo Sarah J. Kester Victor J. Mahaffey Scott A. Hammel Katelyn K. Heaser Christopher D. Hofich Craig D. Tauscher Sarah E. Kerr Jennifer L. Oliveira Eapen K. Jacob Ann M. Moyer Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? Journal of Personalized Medicine transfusion genetic testing leukoreduction transfusion-associated microchimerism interference |
| title | Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? |
| title_full | Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? |
| title_fullStr | Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? |
| title_full_unstemmed | Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? |
| title_short | Does Transfusion of Red Blood Cells Impact Germline Genetic Test Results? |
| title_sort | does transfusion of red blood cells impact germline genetic test results |
| topic | transfusion genetic testing leukoreduction transfusion-associated microchimerism interference |
| url | https://www.mdpi.com/2075-4426/10/4/268 |
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