In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
Abstract Background Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem ba...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-023-03957-2 |
| _version_ | 1797789949504782336 |
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| author | Kevine Johane Jumeta Dongmo Mariscal Brice Tchatat Tali Yannick Stéphane Fotsing Fongang Pierre Leonel K. Tafokeu Taguimjeu Donald Ulrich Kenou Kagho Gabin Thierry Bitchagno Bruno Ndjakou Lenta Fabrice Fekam Boyom Norbert Sewald Silvère Augustin Ngouela |
| author_facet | Kevine Johane Jumeta Dongmo Mariscal Brice Tchatat Tali Yannick Stéphane Fotsing Fongang Pierre Leonel K. Tafokeu Taguimjeu Donald Ulrich Kenou Kagho Gabin Thierry Bitchagno Bruno Ndjakou Lenta Fabrice Fekam Boyom Norbert Sewald Silvère Augustin Ngouela |
| author_sort | Kevine Johane Jumeta Dongmo |
| collection | DOAJ |
| description | Abstract Background Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem bark of D. edulis with an emphasis on the antiplasmodial and cytotoxic effects of extracts, fractions, and isolated compounds. Methods Extracts, fractions, and some isolated compounds were subjected to antiplasmodial activity screening in vitro against chloroquine-sensitive 3D7 and multidrug resistant Dd2 strains of Plasmodium falciparum using a SyBr Green fluorescence-based assay. The cytotoxicity of active extracts, fractions, and compounds was tested against mammalian Raw cell lines using an in vitro resazurin-based viability assay. The structures of the compounds were determined based on their NMR and MS data. The in vivo toxicity using female BALB/c mice was performed on the most active extract according to the protocol of OECD (2002), guideline 423. Results The hydroethanolic extract from the leaves of D. edulis displayed good antiplasmodial activity with IC50 values of 3.10 and 3.56 μg/mL respectively on sensitive (3D7) and multiresistant (Dd2) strains of P. falciparum. Of the sixteen compounds isolated, 3,3′,4-tri-O-methylellagic acid (4) exhibited the highest antiplasmodial activity against PfDd2 strains with an IC50 value of 0.63 μg/mL. All extracts, fractions, and isolated compounds demonstrated no cytotoxicity against Raw cell lines with CC50 > 250 μg/mL. In addition, the most active extract on both strains of P. falciparum was nontoxic in vivo, with a LD50 greater than 2000 and 5000 mg/kg. A phytochemical investigation of the stem bark and leaves of D. edulis afforded sixteen compounds, including two xanthones (1–2), three ellagic acid derivatives (3–5), one phenolic compound (6), one depside (7), one triglyceride (8), one auranthiamide acetate (9), one gallic acid derivative (10), four triterpenoids (11–14), and two steroids (15–16). Compounds 1, 2, 5, 7, 8, and 9 were herein reported for the first time from the Burseraceae family. Conclusion This work highlights the good in vitro antiplasmodial potency of the hydroethanolic extract of the leaves of this plant and that of two isolated constituents (3,3′,4-tri-O-methylellagic acid and ethylgallate) from the plant. These biological results support the use of D. edulis in traditional medicine against malaria. |
| first_indexed | 2024-03-13T01:57:56Z |
| format | Article |
| id | doaj.art-f8bccf5953564d7193faa83849e0b462 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-13T01:57:56Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-f8bccf5953564d7193faa83849e0b4622023-07-02T11:07:04ZengBMCBMC Complementary Medicine and Therapies2662-76712023-06-0123111310.1186/s12906-023-03957-2In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)Kevine Johane Jumeta Dongmo0Mariscal Brice Tchatat Tali1Yannick Stéphane Fotsing Fongang2Pierre Leonel K. Tafokeu Taguimjeu3Donald Ulrich Kenou Kagho4Gabin Thierry Bitchagno5Bruno Ndjakou Lenta6Fabrice Fekam Boyom7Norbert Sewald8Silvère Augustin Ngouela9Department of Organic Chemistry, Faculty of Science, University of Yaoundé IAntimicrobial and Biocontrol Agents Unit, Faculty of Science, University of Yaoundé IDepartment of Chemistry, Higher Teachers’ Training College, The University of MarouaDepartment of Organic Chemistry, Faculty of Science, University of Yaoundé IDepartment of Organic Chemistry, Faculty of Science, University of Yaoundé IDepartment of Chemistry, University of DschangDepartment of Chemistry, Higher Teacher Training College, University of Yaoundé IAntimicrobial and Biocontrol Agents Unit, Faculty of Science, University of Yaoundé IDepartment of Chemistry, Bielefeld UniversityDepartment of Organic Chemistry, Faculty of Science, University of Yaoundé IAbstract Background Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem bark of D. edulis with an emphasis on the antiplasmodial and cytotoxic effects of extracts, fractions, and isolated compounds. Methods Extracts, fractions, and some isolated compounds were subjected to antiplasmodial activity screening in vitro against chloroquine-sensitive 3D7 and multidrug resistant Dd2 strains of Plasmodium falciparum using a SyBr Green fluorescence-based assay. The cytotoxicity of active extracts, fractions, and compounds was tested against mammalian Raw cell lines using an in vitro resazurin-based viability assay. The structures of the compounds were determined based on their NMR and MS data. The in vivo toxicity using female BALB/c mice was performed on the most active extract according to the protocol of OECD (2002), guideline 423. Results The hydroethanolic extract from the leaves of D. edulis displayed good antiplasmodial activity with IC50 values of 3.10 and 3.56 μg/mL respectively on sensitive (3D7) and multiresistant (Dd2) strains of P. falciparum. Of the sixteen compounds isolated, 3,3′,4-tri-O-methylellagic acid (4) exhibited the highest antiplasmodial activity against PfDd2 strains with an IC50 value of 0.63 μg/mL. All extracts, fractions, and isolated compounds demonstrated no cytotoxicity against Raw cell lines with CC50 > 250 μg/mL. In addition, the most active extract on both strains of P. falciparum was nontoxic in vivo, with a LD50 greater than 2000 and 5000 mg/kg. A phytochemical investigation of the stem bark and leaves of D. edulis afforded sixteen compounds, including two xanthones (1–2), three ellagic acid derivatives (3–5), one phenolic compound (6), one depside (7), one triglyceride (8), one auranthiamide acetate (9), one gallic acid derivative (10), four triterpenoids (11–14), and two steroids (15–16). Compounds 1, 2, 5, 7, 8, and 9 were herein reported for the first time from the Burseraceae family. Conclusion This work highlights the good in vitro antiplasmodial potency of the hydroethanolic extract of the leaves of this plant and that of two isolated constituents (3,3′,4-tri-O-methylellagic acid and ethylgallate) from the plant. These biological results support the use of D. edulis in traditional medicine against malaria.https://doi.org/10.1186/s12906-023-03957-2Dacryodes edulisBurseraceaeAntiplasmodialAcute toxicityCytotoxicity3,3′,4-tri-O-methylellagic acid |
| spellingShingle | Kevine Johane Jumeta Dongmo Mariscal Brice Tchatat Tali Yannick Stéphane Fotsing Fongang Pierre Leonel K. Tafokeu Taguimjeu Donald Ulrich Kenou Kagho Gabin Thierry Bitchagno Bruno Ndjakou Lenta Fabrice Fekam Boyom Norbert Sewald Silvère Augustin Ngouela In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) BMC Complementary Medicine and Therapies Dacryodes edulis Burseraceae Antiplasmodial Acute toxicity Cytotoxicity 3,3′,4-tri-O-methylellagic acid |
| title | In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) |
| title_full | In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) |
| title_fullStr | In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) |
| title_full_unstemmed | In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) |
| title_short | In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) |
| title_sort | in vitro antiplasmodial activity and toxicological profile of extracts fractions and chemical constituents of leaves and stem bark from dacryodes edulis burseraceae |
| topic | Dacryodes edulis Burseraceae Antiplasmodial Acute toxicity Cytotoxicity 3,3′,4-tri-O-methylellagic acid |
| url | https://doi.org/10.1186/s12906-023-03957-2 |
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