Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min...
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MDPI AG
2016-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/12/1739 |
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| author | Virginia Chamorro Rachele Pandolfi Laura Moreno Bianca Barreira Andrea Martínez-Ramas Daniel Morales-Cano Jesús Ruiz-Cabello José Angel Lorente Juan Duarte Ángel Cogolludo José Luis Alvarez-Sala Francisco Perez-Vizcaino |
| author_facet | Virginia Chamorro Rachele Pandolfi Laura Moreno Bianca Barreira Andrea Martínez-Ramas Daniel Morales-Cano Jesús Ruiz-Cabello José Angel Lorente Juan Duarte Ángel Cogolludo José Luis Alvarez-Sala Francisco Perez-Vizcaino |
| author_sort | Virginia Chamorro |
| collection | DOAJ |
| description | Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion. |
| first_indexed | 2024-04-12T17:02:53Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T17:02:53Z |
| publishDate | 2016-12-01 |
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| series | Molecules |
| spelling | doaj.art-f8be5daa58a54540a191645e52eaa8402022-12-22T03:24:02ZengMDPI AGMolecules1420-30492016-12-012112173910.3390/molecules21121739molecules21121739Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and ReperfusionVirginia Chamorro0Rachele Pandolfi1Laura Moreno2Bianca Barreira3Andrea Martínez-Ramas4Daniel Morales-Cano5Jesús Ruiz-Cabello6José Angel Lorente7Juan Duarte8Ángel Cogolludo9José Luis Alvarez-Sala10Francisco Perez-Vizcaino11Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainDepartamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainDepartamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainCentro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, SpainCentro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, SpainDepartamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainCentro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, SpainCentro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, SpainDepartamento de Farmacología, Facultad de Farmacia, Universidad de Granada, Granada 18071, SpainDepartamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainDepartamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainDepartamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, SpainBackground: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion.http://www.mdpi.com/1420-3049/21/12/1739systemic arterial pressurelung edemahemorrhagetraumareperfusion |
| spellingShingle | Virginia Chamorro Rachele Pandolfi Laura Moreno Bianca Barreira Andrea Martínez-Ramas Daniel Morales-Cano Jesús Ruiz-Cabello José Angel Lorente Juan Duarte Ángel Cogolludo José Luis Alvarez-Sala Francisco Perez-Vizcaino Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion Molecules systemic arterial pressure lung edema hemorrhage trauma reperfusion |
| title | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
| title_full | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
| title_fullStr | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
| title_full_unstemmed | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
| title_short | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
| title_sort | effects of quercetin in a rat model of hemorrhagic traumatic shock and reperfusion |
| topic | systemic arterial pressure lung edema hemorrhage trauma reperfusion |
| url | http://www.mdpi.com/1420-3049/21/12/1739 |
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