Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants
This work presents the synthesis of nanostructured TiO<sub>2</sub> modified with noble metal nanoparticles (Au, Ag) and lysozyme and coated on titanium foil. Moreover, the specific structural and functional properties of the resulting inorganic and hybrid materials were explored. The pur...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/18/3186 |
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| author | Emilian Chifor Ion Bordeianu Crina Anastasescu Jose Maria Calderon-Moreno Veronica Bratan Diana-Ioana Eftemie Mihai Anastasescu Silviu Preda Gabriel Plavan Diana Pelinescu Robertina Ionescu Ileana Stoica Maria Zaharescu Ioan Balint |
| author_facet | Emilian Chifor Ion Bordeianu Crina Anastasescu Jose Maria Calderon-Moreno Veronica Bratan Diana-Ioana Eftemie Mihai Anastasescu Silviu Preda Gabriel Plavan Diana Pelinescu Robertina Ionescu Ileana Stoica Maria Zaharescu Ioan Balint |
| author_sort | Emilian Chifor |
| collection | DOAJ |
| description | This work presents the synthesis of nanostructured TiO<sub>2</sub> modified with noble metal nanoparticles (Au, Ag) and lysozyme and coated on titanium foil. Moreover, the specific structural and functional properties of the resulting inorganic and hybrid materials were explored. The purpose of this study was to identify the key parameters for developing engineered coatings on titanium foil appropriate for efficient dental implants with intrinsic antibacterial activity. TiO<sub>2</sub> nanoparticles obtained using the sol–gel method were deposited on Ti foil and modified with Au/Ag nanoparticles. Morphological and structural investigations (scanning electron and atomic force microscopies, X-ray diffraction, photoluminescence, and UV–Vis spectroscopies) were carried out for the characterization of the resulting inorganic coatings. In order to modify their antibacterial activity, which is essential for safe dental implants, the following aspects were investigated: (a) singlet oxygen (<sup>1</sup>O<sub>2</sub>) generation by inorganic coatings exposed to visible light irradiation; (b) the antibacterial behavior emphasized by titania-based coatings deposited on titanium foil (TiO<sub>2</sub>/Ti foil; Au–TiO<sub>2</sub>/Ti foil, Ag–TiO<sub>2</sub>/Ti foil); (c) the lysozyme bioactivity on the microbial substrate <i>(Micrococcus lysodeicticus</i>) after its adsorption on inorganic surfaces (Lys/TiO<sub>2</sub>/Ti foil; Lys/Au–TiO<sub>2</sub>/Ti foil, Lys/Ag–TiO<sub>2</sub>/Ti foil); (d) the enzymatic activity of the above-mentioned hybrids materials for the hydrolysis reaction of a synthetic organic substrate usually used for monitoring the lysozyme biocatalytic activity, namely, 4-Methylumbelliferyl β-D-N,N′,N″-triacetylchitotrioside [4-MU-β- (GlcNAc)<sub>3</sub>]. This was evaluated by identifying the presence of a fluorescent reaction product, 7-hydroxy-4-metyl coumarin (4-methylumbelliferone). |
| first_indexed | 2024-03-09T22:58:39Z |
| format | Article |
| id | doaj.art-f8c128153c8e4116a7dc4501b2e09dec |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T22:58:39Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-f8c128153c8e4116a7dc4501b2e09dec2023-11-23T18:07:00ZengMDPI AGNanomaterials2079-49912022-09-011218318610.3390/nano12183186Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental ImplantsEmilian Chifor0Ion Bordeianu1Crina Anastasescu2Jose Maria Calderon-Moreno3Veronica Bratan4Diana-Ioana Eftemie5Mihai Anastasescu6Silviu Preda7Gabriel Plavan8Diana Pelinescu9Robertina Ionescu10Ileana Stoica11Maria Zaharescu12Ioan Balint13Faculty of Medicine of the Ovidius University, Aleea Universitatii nr.1, 900470 Constanţa, RomaniaFaculty of Medicine of the Ovidius University, Aleea Universitatii nr.1, 900470 Constanţa, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaFaculty of Biology, “Alexandru Ioan Cuza” University, 700505 Iasi, RomaniaFaculty of Biology, Intrarea Portocalilor 1-3, Sector 5, 060101 Bucharest, RomaniaFaculty of Biology, Intrarea Portocalilor 1-3, Sector 5, 060101 Bucharest, RomaniaFaculty of Biology, Intrarea Portocalilor 1-3, Sector 5, 060101 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, RomaniaThis work presents the synthesis of nanostructured TiO<sub>2</sub> modified with noble metal nanoparticles (Au, Ag) and lysozyme and coated on titanium foil. Moreover, the specific structural and functional properties of the resulting inorganic and hybrid materials were explored. The purpose of this study was to identify the key parameters for developing engineered coatings on titanium foil appropriate for efficient dental implants with intrinsic antibacterial activity. TiO<sub>2</sub> nanoparticles obtained using the sol–gel method were deposited on Ti foil and modified with Au/Ag nanoparticles. Morphological and structural investigations (scanning electron and atomic force microscopies, X-ray diffraction, photoluminescence, and UV–Vis spectroscopies) were carried out for the characterization of the resulting inorganic coatings. In order to modify their antibacterial activity, which is essential for safe dental implants, the following aspects were investigated: (a) singlet oxygen (<sup>1</sup>O<sub>2</sub>) generation by inorganic coatings exposed to visible light irradiation; (b) the antibacterial behavior emphasized by titania-based coatings deposited on titanium foil (TiO<sub>2</sub>/Ti foil; Au–TiO<sub>2</sub>/Ti foil, Ag–TiO<sub>2</sub>/Ti foil); (c) the lysozyme bioactivity on the microbial substrate <i>(Micrococcus lysodeicticus</i>) after its adsorption on inorganic surfaces (Lys/TiO<sub>2</sub>/Ti foil; Lys/Au–TiO<sub>2</sub>/Ti foil, Lys/Ag–TiO<sub>2</sub>/Ti foil); (d) the enzymatic activity of the above-mentioned hybrids materials for the hydrolysis reaction of a synthetic organic substrate usually used for monitoring the lysozyme biocatalytic activity, namely, 4-Methylumbelliferyl β-D-N,N′,N″-triacetylchitotrioside [4-MU-β- (GlcNAc)<sub>3</sub>]. This was evaluated by identifying the presence of a fluorescent reaction product, 7-hydroxy-4-metyl coumarin (4-methylumbelliferone).https://www.mdpi.com/2079-4991/12/18/3186TiO<sub>2</sub> coatings for titanium implantnoble metal nanoparticlesphotosensitive materialsantibacterial activityhybrid materialsbiocatalytic activity of lysozyme |
| spellingShingle | Emilian Chifor Ion Bordeianu Crina Anastasescu Jose Maria Calderon-Moreno Veronica Bratan Diana-Ioana Eftemie Mihai Anastasescu Silviu Preda Gabriel Plavan Diana Pelinescu Robertina Ionescu Ileana Stoica Maria Zaharescu Ioan Balint Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants Nanomaterials TiO<sub>2</sub> coatings for titanium implant noble metal nanoparticles photosensitive materials antibacterial activity hybrid materials biocatalytic activity of lysozyme |
| title | Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants |
| title_full | Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants |
| title_fullStr | Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants |
| title_full_unstemmed | Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants |
| title_short | Bioactive Coatings Based on Nanostructured TiO<sub>2</sub> Modified with Noble Metal Nanoparticles and Lysozyme for Ti Dental Implants |
| title_sort | bioactive coatings based on nanostructured tio sub 2 sub modified with noble metal nanoparticles and lysozyme for ti dental implants |
| topic | TiO<sub>2</sub> coatings for titanium implant noble metal nanoparticles photosensitive materials antibacterial activity hybrid materials biocatalytic activity of lysozyme |
| url | https://www.mdpi.com/2079-4991/12/18/3186 |
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