Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture
Abstract The incidence of chorioamnionitis and neonatal sepsis increases with the increasing time of rupture of membranes. Changes in the amount and categories of microbiomes in maternal and fetal environments after membrane rupture have yet to be discussed. In order to determine the microbiome dive...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41777-z |
| _version_ | 1827723206478266368 |
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| author | Huifen Yin Jiao Yu Wei Wu Xiaotian Li Rong Hu |
| author_facet | Huifen Yin Jiao Yu Wei Wu Xiaotian Li Rong Hu |
| author_sort | Huifen Yin |
| collection | DOAJ |
| description | Abstract The incidence of chorioamnionitis and neonatal sepsis increases with the increasing time of rupture of membranes. Changes in the amount and categories of microbiomes in maternal and fetal environments after membrane rupture have yet to be discussed. In order to determine the microbiome diversity and signature in the maternal, intrauterine, and fetal environments of different durations following membrane rupture, we collected samples of fetal membrane, amniotic fluid, cord blood and maternal peripheral blood from singleton pregnant women and divided them into five groups according to the duration of membrane rupture. DNA was isolated from the samples, and the V3V4 region of bacterial 16S rRNA genes was sequenced. We found that the alpha diversity of the fetal membrane microbiome increased significantly 12 h after membrane rupture, while the beta diversity of the amniotic fluid microbiome increased 24 h after membrane rupture. In cord blood, the mean proportion of Methylobacterium and Halomonadaceae reached the highest 12 h after membrane rupture, and the mean proportion of Prevotella reached the highest 24 h after membrane rupture. The LEfSe algorithm showed that Ruminococcus, Paludibaculum, Lachnospiraceae, and Prevotella were detected earlier in cord blood or maternal blood and then detected in fetal membranes or amniotic fluid, which may suggest a reverse infection model. In conclusion, the microbes may invade the placenta 12 h after membrane rupture and invaded the amniotic cavity 24 h after membrane rupture. In addition to the common ascending pattern of infection, the hematogenous pathway of intrauterine infection should also be considered among people with rupture of membranes. |
| first_indexed | 2024-03-10T21:56:06Z |
| format | Article |
| id | doaj.art-f8c1314d001c4d689913326b1d3ca67c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:56:06Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f8c1314d001c4d689913326b1d3ca67c2023-11-19T13:09:07ZengNature PortfolioScientific Reports2045-23222023-09-0113111010.1038/s41598-023-41777-zAnalysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane ruptureHuifen Yin0Jiao Yu1Wei Wu2Xiaotian Li3Rong Hu4The Obstetrics and Gynecology Hospital of Fudan UniversityThe Obstetrics and Gynecology Hospital of Fudan UniversityThe Obstetrics and Gynecology Hospital of Fudan UniversityThe Obstetrics and Gynecology Hospital of Fudan UniversityThe Obstetrics and Gynecology Hospital of Fudan UniversityAbstract The incidence of chorioamnionitis and neonatal sepsis increases with the increasing time of rupture of membranes. Changes in the amount and categories of microbiomes in maternal and fetal environments after membrane rupture have yet to be discussed. In order to determine the microbiome diversity and signature in the maternal, intrauterine, and fetal environments of different durations following membrane rupture, we collected samples of fetal membrane, amniotic fluid, cord blood and maternal peripheral blood from singleton pregnant women and divided them into five groups according to the duration of membrane rupture. DNA was isolated from the samples, and the V3V4 region of bacterial 16S rRNA genes was sequenced. We found that the alpha diversity of the fetal membrane microbiome increased significantly 12 h after membrane rupture, while the beta diversity of the amniotic fluid microbiome increased 24 h after membrane rupture. In cord blood, the mean proportion of Methylobacterium and Halomonadaceae reached the highest 12 h after membrane rupture, and the mean proportion of Prevotella reached the highest 24 h after membrane rupture. The LEfSe algorithm showed that Ruminococcus, Paludibaculum, Lachnospiraceae, and Prevotella were detected earlier in cord blood or maternal blood and then detected in fetal membranes or amniotic fluid, which may suggest a reverse infection model. In conclusion, the microbes may invade the placenta 12 h after membrane rupture and invaded the amniotic cavity 24 h after membrane rupture. In addition to the common ascending pattern of infection, the hematogenous pathway of intrauterine infection should also be considered among people with rupture of membranes.https://doi.org/10.1038/s41598-023-41777-z |
| spellingShingle | Huifen Yin Jiao Yu Wei Wu Xiaotian Li Rong Hu Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture Scientific Reports |
| title | Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture |
| title_full | Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture |
| title_fullStr | Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture |
| title_full_unstemmed | Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture |
| title_short | Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture |
| title_sort | analysis of the microbiome in maternal intrauterine and fetal environments based on 16s rrna genes following different durations of membrane rupture |
| url | https://doi.org/10.1038/s41598-023-41777-z |
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