Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application
Carbon Dots (CDs) were synthesized using laser ablation by focusing the laser beam on carbon (Tea) material in colloid (CH3) for 3 hours. UV-Vis spectroscopic and fluorometric characterization showed absorption of the wavelength peaks caused by the control treatment and after laser ablation and coat...
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| Format: | Article |
| Language: | English |
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Jurusan Fisika, FMIPA Universitas Andalas
2023-05-01
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| Series: | JIF (Jurnal Ilmu Fisika) |
| Subjects: | |
| Online Access: | http://jif.fmipa.unand.ac.id/index.php/jif/article/view/536 |
| _version_ | 1797831856353181696 |
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| author | Jumardin Jumardin Akhiruddin Maddu Kokoeh Santoso Isnaeni Isnaeni |
| author_facet | Jumardin Jumardin Akhiruddin Maddu Kokoeh Santoso Isnaeni Isnaeni |
| author_sort | Jumardin Jumardin |
| collection | DOAJ |
| description | Carbon Dots (CDs) were synthesized using laser ablation by focusing the laser beam on carbon (Tea) material in colloid (CH3) for 3 hours. UV-Vis spectroscopic and fluorometric characterization showed absorption of the wavelength peaks caused by the control treatment and after laser ablation and coating using Poly Ethylene Glycol (PEG400). The excitation and emission energies are formulations of CDs absorbance wavelength and fluorescence intensity. The absorbance coefficient is obtained based on the absorbance value of the cuvette thickness. The transmittance value (T) is obtained based on the absorption coefficient multiplied by 100%. CD fluorescence wavelength based on control parameters was 489 nm. After laser ablation was 496 nm, and after coating was 511 nm. CDs morphology and size characteristics are 4 nm to 10 nm based on TEM measurements. Fluorescence analysis for bioimaging applications on the luminescence intensity value of internalized blue CDs in zebrafish eye organs. The average intensity of CDs in the eye organs, gill, intestinal, dorsal, and tail injection points was 88.15 %, 91.58 %, 92.76 %, and 0.00 %.
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| first_indexed | 2024-04-09T13:58:25Z |
| format | Article |
| id | doaj.art-f8c424855cfe49c28b0e0378c1d8c268 |
| institution | Directory Open Access Journal |
| issn | 1979-4657 2614-7386 |
| language | English |
| last_indexed | 2024-04-09T13:58:25Z |
| publishDate | 2023-05-01 |
| publisher | Jurusan Fisika, FMIPA Universitas Andalas |
| record_format | Article |
| series | JIF (Jurnal Ilmu Fisika) |
| spelling | doaj.art-f8c424855cfe49c28b0e0378c1d8c2682023-05-07T13:31:52ZengJurusan Fisika, FMIPA Universitas AndalasJIF (Jurnal Ilmu Fisika)1979-46572614-73862023-05-0115210.25077/jif.15.2.91-105.2023Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging ApplicationJumardin Jumardin0Akhiruddin Maddu1Kokoeh Santoso2Isnaeni Isnaeni3Department of Physics, Faculty of Sciences and Technology, Alauddin State Islamic University of Makassar Gowa, 92113Department of Physics, Faculty of Mathematics and Natural Sciences, IPB University, Bogor, 16680Department of Anatomy, Physiology and Pharmacology, School of Veterinary and Biomedical, IPB University Bogor, 16680Research Center for Photonics, National Research and Innovation Agency (Badan Riset dan Inovasi Nasional, BRIN) BJ Habibie Science and Technology Park, Banten 15314Carbon Dots (CDs) were synthesized using laser ablation by focusing the laser beam on carbon (Tea) material in colloid (CH3) for 3 hours. UV-Vis spectroscopic and fluorometric characterization showed absorption of the wavelength peaks caused by the control treatment and after laser ablation and coating using Poly Ethylene Glycol (PEG400). The excitation and emission energies are formulations of CDs absorbance wavelength and fluorescence intensity. The absorbance coefficient is obtained based on the absorbance value of the cuvette thickness. The transmittance value (T) is obtained based on the absorption coefficient multiplied by 100%. CD fluorescence wavelength based on control parameters was 489 nm. After laser ablation was 496 nm, and after coating was 511 nm. CDs morphology and size characteristics are 4 nm to 10 nm based on TEM measurements. Fluorescence analysis for bioimaging applications on the luminescence intensity value of internalized blue CDs in zebrafish eye organs. The average intensity of CDs in the eye organs, gill, intestinal, dorsal, and tail injection points was 88.15 %, 91.58 %, 92.76 %, and 0.00 %. http://jif.fmipa.unand.ac.id/index.php/jif/article/view/536Carbon dotsLaser ablationFluoresccentBioimaging |
| spellingShingle | Jumardin Jumardin Akhiruddin Maddu Kokoeh Santoso Isnaeni Isnaeni Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application JIF (Jurnal Ilmu Fisika) Carbon dots Laser ablation Fluoresccent Bioimaging |
| title | Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application |
| title_full | Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application |
| title_fullStr | Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application |
| title_full_unstemmed | Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application |
| title_short | Synthesis of Fluorescent Carbon Dots (CDs) Using Laser Ablation Method for Bioimaging Application |
| title_sort | synthesis of fluorescent carbon dots cds using laser ablation method for bioimaging application |
| topic | Carbon dots Laser ablation Fluoresccent Bioimaging |
| url | http://jif.fmipa.unand.ac.id/index.php/jif/article/view/536 |
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