Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent
The consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for <i>Morus alba</i> callus extraction. The efficiency of extraction from t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Cosmetics |
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| Online Access: | https://www.mdpi.com/2079-9284/8/3/91 |
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| author | Jukrapun Komaikul Supachoke Mangmool Waraporn Putalun Tharita Kitisripanya |
| author_facet | Jukrapun Komaikul Supachoke Mangmool Waraporn Putalun Tharita Kitisripanya |
| author_sort | Jukrapun Komaikul |
| collection | DOAJ |
| description | The consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for <i>Morus alba</i> callus extraction. The efficiency of extraction from the NADES and methanol was investigated by comparison of the stilbenoids yield and anti-melanogenesis activity. Prior to testing the irritability of a suitable NADES on the reconstructed human epidermis (RhE), the effect of the selected NADES on stilbenoids stability was determined. The results showed that the highest yields of stilbenoids were obtained from choline chloride-glycerol mixtures (Ch1G2) and methanol extracts, with no significant difference in yields (5.06 ± 0.05 and 6.32 ± 0.40 mg/g callus dry weight, respectively). The NADES extracts of <i>M. alba</i> callus showed comparable anti-melanogenesis activity compared to methanol. In term of stability, stilbenoids in Ch1G2 remained stable after six months of storage at 4 °C except resveratrol. Furthermore, Ch1G2 had no irritation effect on RhE. Thus, based on the findings of this study, Ch1G2 is an intriguing green solvent alternative for the extraction of <i>M. alba</i> callus and may be advantageous for the preparation of skin-lightening cosmetics. |
| first_indexed | 2024-03-10T07:47:56Z |
| format | Article |
| id | doaj.art-f8c5e39a9dae4689820e5223e0d5acc0 |
| institution | Directory Open Access Journal |
| issn | 2079-9284 |
| language | English |
| last_indexed | 2024-03-10T07:47:56Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cosmetics |
| spelling | doaj.art-f8c5e39a9dae4689820e5223e0d5acc02023-11-22T12:34:20ZengMDPI AGCosmetics2079-92842021-09-01839110.3390/cosmetics8030091Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic SolventJukrapun Komaikul0Supachoke Mangmool1Waraporn Putalun2Tharita Kitisripanya3Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, ThailandDepartment of Pharmacology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, ThailandThe consumer and cosmetic industries have recently placed a greater emphasis on ecofriendly solvents for botanical extraction, including natural deep eutectic solvents (NADES). In this study, NADES were prepared for <i>Morus alba</i> callus extraction. The efficiency of extraction from the NADES and methanol was investigated by comparison of the stilbenoids yield and anti-melanogenesis activity. Prior to testing the irritability of a suitable NADES on the reconstructed human epidermis (RhE), the effect of the selected NADES on stilbenoids stability was determined. The results showed that the highest yields of stilbenoids were obtained from choline chloride-glycerol mixtures (Ch1G2) and methanol extracts, with no significant difference in yields (5.06 ± 0.05 and 6.32 ± 0.40 mg/g callus dry weight, respectively). The NADES extracts of <i>M. alba</i> callus showed comparable anti-melanogenesis activity compared to methanol. In term of stability, stilbenoids in Ch1G2 remained stable after six months of storage at 4 °C except resveratrol. Furthermore, Ch1G2 had no irritation effect on RhE. Thus, based on the findings of this study, Ch1G2 is an intriguing green solvent alternative for the extraction of <i>M. alba</i> callus and may be advantageous for the preparation of skin-lightening cosmetics.https://www.mdpi.com/2079-9284/8/3/91natural deep eutectic solvent<i>Morus alba</i>stilbenoidsstability |
| spellingShingle | Jukrapun Komaikul Supachoke Mangmool Waraporn Putalun Tharita Kitisripanya Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent Cosmetics natural deep eutectic solvent <i>Morus alba</i> stilbenoids stability |
| title | Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent |
| title_full | Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent |
| title_fullStr | Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent |
| title_full_unstemmed | Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent |
| title_short | Preparation of Readily-to-Use Stilbenoids Extract from <i>Morus alba</i> Callus Using a Natural Deep Eutectic Solvent |
| title_sort | preparation of readily to use stilbenoids extract from i morus alba i callus using a natural deep eutectic solvent |
| topic | natural deep eutectic solvent <i>Morus alba</i> stilbenoids stability |
| url | https://www.mdpi.com/2079-9284/8/3/91 |
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