A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy
<i>Helicobacter pylori</i> (<i>Hp</i>) eradication therapy alters gut microbiota, provoking gastrointestinal (GI) symptoms that could be improved by probiotics. The study aim was to assess the effect in <i>Hp</i> patients of a Test fermented milk containing yogurt...
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/2072-6643/13/9/3171 |
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| author | Eric Guillemard Marion Poirel Florent Schäfer Laurent Quinquis Caroline Rossoni Christian Keicher Frank Wagner Hania Szajewska Frédéric Barbut Muriel Derrien Peter Malfertheiner |
| author_facet | Eric Guillemard Marion Poirel Florent Schäfer Laurent Quinquis Caroline Rossoni Christian Keicher Frank Wagner Hania Szajewska Frédéric Barbut Muriel Derrien Peter Malfertheiner |
| author_sort | Eric Guillemard |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> (<i>Hp</i>) eradication therapy alters gut microbiota, provoking gastrointestinal (GI) symptoms that could be improved by probiotics. The study aim was to assess the effect in <i>Hp</i> patients of a Test fermented milk containing yogurt and <i>Lacticaseibacillus</i> (<i>L. paracasei</i> CNCM I-1518 and I-3689, <i>L. rhamnosus</i> CNCM I-3690) strains on antibiotic associated diarrhea (AAD) (primary aim), GI-symptoms, gut microbiota, and metabolites. A randomised, double-blind, controlled trial was performed on 136 adults under 14-day <i>Hp</i> treatment, receiving the Test or Control product for 28 days. AAD and GI-symptoms were reported and feces analysed for relative and quantitative gut microbiome composition, short chain fatty acids (SCFA), and calprotectin concentrations, and viability of ingested strains. No effect of Test product was observed on AAD or GI-symptoms. <i>Hp</i> treatment induced a significant alteration in bacterial and fungal composition, a decrease of bacterial count and alpha-diversity, an increase of <i>Candida</i> and calprotectin, and a decrease of SCFA concentrations. Following <i>Hp</i> treatment, in the Test as compared to Control group, intra-subject beta-diversity distance from baseline was lower (<i>p</i><sub>adj</sub> = 0.02), some <i>Enterobacteriaceae</i>, including <i>Escherichia-Shigella</i> (<i>p</i><sub>adj</sub> = 0.0082) and <i>Klebsiella</i> (<i>p</i><sub>adj</sub> = 0.013), were less abundant, and concentrations of major SCFA (<i>p</i> = 0.035) and valerate (<i>p</i> = 0.045) were higher. Viable <i>Lacticaseibacillus</i> strains were detected during product consumption in feces. Results suggest that, in patients under <i>Hp</i> treatment, the consumption of a multi-strain fermented milk can induce a modest but significant faster recovery of the microbiota composition (beta-diversity) and of SCFA production and limit the increase of potentially pathogenic bacteria. |
| first_indexed | 2024-03-10T07:20:49Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T07:20:49Z |
| publishDate | 2021-09-01 |
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| spelling | doaj.art-f8c61f0dc92b41b49e3401f015c7c32a2023-11-22T14:38:33ZengMDPI AGNutrients2072-66432021-09-01139317110.3390/nu13093171A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> TherapyEric Guillemard0Marion Poirel1Florent Schäfer2Laurent Quinquis3Caroline Rossoni4Christian Keicher5Frank Wagner6Hania Szajewska7Frédéric Barbut8Muriel Derrien9Peter Malfertheiner10Danone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceDanone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceDanone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceDanone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceDanone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceCharité Research Organisation GmbH, 10117 Berlin, GermanyCharité Research Organisation GmbH, 10117 Berlin, GermanyDepartment of Paediatrics, Medical University of Warsaw, 02-091 Warszawa, PolandINSERM S-1139, Université de Paris, 75006 Paris, FranceDanone Nutricia Research, Department of Innovation Science and Nutrition, 91767 Palaiseau, FranceDepartment of Gastroenterology, Hepatology and Infectious Diseases, Magdeburg Clinic, OVGU University, 39120 Magdeburg, Germany<i>Helicobacter pylori</i> (<i>Hp</i>) eradication therapy alters gut microbiota, provoking gastrointestinal (GI) symptoms that could be improved by probiotics. The study aim was to assess the effect in <i>Hp</i> patients of a Test fermented milk containing yogurt and <i>Lacticaseibacillus</i> (<i>L. paracasei</i> CNCM I-1518 and I-3689, <i>L. rhamnosus</i> CNCM I-3690) strains on antibiotic associated diarrhea (AAD) (primary aim), GI-symptoms, gut microbiota, and metabolites. A randomised, double-blind, controlled trial was performed on 136 adults under 14-day <i>Hp</i> treatment, receiving the Test or Control product for 28 days. AAD and GI-symptoms were reported and feces analysed for relative and quantitative gut microbiome composition, short chain fatty acids (SCFA), and calprotectin concentrations, and viability of ingested strains. No effect of Test product was observed on AAD or GI-symptoms. <i>Hp</i> treatment induced a significant alteration in bacterial and fungal composition, a decrease of bacterial count and alpha-diversity, an increase of <i>Candida</i> and calprotectin, and a decrease of SCFA concentrations. Following <i>Hp</i> treatment, in the Test as compared to Control group, intra-subject beta-diversity distance from baseline was lower (<i>p</i><sub>adj</sub> = 0.02), some <i>Enterobacteriaceae</i>, including <i>Escherichia-Shigella</i> (<i>p</i><sub>adj</sub> = 0.0082) and <i>Klebsiella</i> (<i>p</i><sub>adj</sub> = 0.013), were less abundant, and concentrations of major SCFA (<i>p</i> = 0.035) and valerate (<i>p</i> = 0.045) were higher. Viable <i>Lacticaseibacillus</i> strains were detected during product consumption in feces. Results suggest that, in patients under <i>Hp</i> treatment, the consumption of a multi-strain fermented milk can induce a modest but significant faster recovery of the microbiota composition (beta-diversity) and of SCFA production and limit the increase of potentially pathogenic bacteria.https://www.mdpi.com/2072-6643/13/9/3171antibiotics<i>Helicobacter pylori</i>antibiotic associated diarrheagut microbiotashort chain fatty acidsrecovery |
| spellingShingle | Eric Guillemard Marion Poirel Florent Schäfer Laurent Quinquis Caroline Rossoni Christian Keicher Frank Wagner Hania Szajewska Frédéric Barbut Muriel Derrien Peter Malfertheiner A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy Nutrients antibiotics <i>Helicobacter pylori</i> antibiotic associated diarrhea gut microbiota short chain fatty acids recovery |
| title | A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy |
| title_full | A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy |
| title_fullStr | A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy |
| title_full_unstemmed | A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy |
| title_short | A Randomised, Controlled Trial: Effect of a Multi-Strain Fermented Milk on the Gut Microbiota Recovery after <i>Helicobacter pylori</i> Therapy |
| title_sort | randomised controlled trial effect of a multi strain fermented milk on the gut microbiota recovery after i helicobacter pylori i therapy |
| topic | antibiotics <i>Helicobacter pylori</i> antibiotic associated diarrhea gut microbiota short chain fatty acids recovery |
| url | https://www.mdpi.com/2072-6643/13/9/3171 |
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