K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells
Adenoviral vectors are important vehicles for delivering therapeutic genes into mammalian cells. However, the yield of the adenoviral transduction of murine mesenchymal stromal cells (MSC) is low. Here, we aimed to improve the adenoviral transduction efficiency of bone marrow-derived MSC. Our data s...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/598 |
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| author | Madalina Dumitrescu Ana Maria Vacaru Violeta Georgeta Trusca Ioana Madalina Fenyo Radu Ionita Anca Violeta Gafencu |
| author_facet | Madalina Dumitrescu Ana Maria Vacaru Violeta Georgeta Trusca Ioana Madalina Fenyo Radu Ionita Anca Violeta Gafencu |
| author_sort | Madalina Dumitrescu |
| collection | DOAJ |
| description | Adenoviral vectors are important vehicles for delivering therapeutic genes into mammalian cells. However, the yield of the adenoviral transduction of murine mesenchymal stromal cells (MSC) is low. Here, we aimed to improve the adenoviral transduction efficiency of bone marrow-derived MSC. Our data showed that among all the potential transduction boosters that we tested, the K2 Transfection System (K2TS) greatly increased the transduction efficiency. After optimization of both K2TS components, the yield of the adenoviral transduction increased from 18% to 96% for non-obese diabetic (NOD)-derived MSC, from 30% to 86% for C57BL/6-derived MSC, and from 0.6% to 63% for BALB/c-derived MSC, when 250 transduction units/cell were used. We found that MSC derived from these mouse strains expressed different levels of the coxsackievirus and adenovirus receptors (MSC from C57BL/6≥NOD>>>BALB/c). K2TS did not increase the level of the receptor expression, but desensitized the cells to foreign DNA and facilitated the virus entry into the cell. The expression of Stem cells antigen-1 (Sca-1) and 5′-nucleotidase (CD73) MSC markers, the adipogenic and osteogenic differentiation potential, and the immunosuppressive capacity were preserved after the adenoviral transduction of MSC in the presence of the K2TS. In conclusion, K2TS significantly enhanced the adenoviral transduction of MSC, without interfering with their main characteristics and properties. |
| first_indexed | 2024-03-09T05:27:53Z |
| format | Article |
| id | doaj.art-f8d0fd6e4b674e5ebaddbff1cd064c24 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:27:53Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f8d0fd6e4b674e5ebaddbff1cd064c242023-12-03T12:35:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122259810.3390/ijms22020598K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal CellsMadalina Dumitrescu0Ana Maria Vacaru1Violeta Georgeta Trusca2Ioana Madalina Fenyo3Radu Ionita4Anca Violeta Gafencu5Institute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaInstitute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaInstitute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaInstitute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaInstitute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaInstitute of Cellular Biology and Pathology “N. Simionescu”, 8, B.P. Hasdeu Street, 050568 Bucharest, RomaniaAdenoviral vectors are important vehicles for delivering therapeutic genes into mammalian cells. However, the yield of the adenoviral transduction of murine mesenchymal stromal cells (MSC) is low. Here, we aimed to improve the adenoviral transduction efficiency of bone marrow-derived MSC. Our data showed that among all the potential transduction boosters that we tested, the K2 Transfection System (K2TS) greatly increased the transduction efficiency. After optimization of both K2TS components, the yield of the adenoviral transduction increased from 18% to 96% for non-obese diabetic (NOD)-derived MSC, from 30% to 86% for C57BL/6-derived MSC, and from 0.6% to 63% for BALB/c-derived MSC, when 250 transduction units/cell were used. We found that MSC derived from these mouse strains expressed different levels of the coxsackievirus and adenovirus receptors (MSC from C57BL/6≥NOD>>>BALB/c). K2TS did not increase the level of the receptor expression, but desensitized the cells to foreign DNA and facilitated the virus entry into the cell. The expression of Stem cells antigen-1 (Sca-1) and 5′-nucleotidase (CD73) MSC markers, the adipogenic and osteogenic differentiation potential, and the immunosuppressive capacity were preserved after the adenoviral transduction of MSC in the presence of the K2TS. In conclusion, K2TS significantly enhanced the adenoviral transduction of MSC, without interfering with their main characteristics and properties.https://www.mdpi.com/1422-0067/22/2/598mesenchymal stromal cellsNOD-derived MSCC57BL/6-derived MSCBALC/c-derived MSCadenovirusK2 transfection system |
| spellingShingle | Madalina Dumitrescu Ana Maria Vacaru Violeta Georgeta Trusca Ioana Madalina Fenyo Radu Ionita Anca Violeta Gafencu K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells International Journal of Molecular Sciences mesenchymal stromal cells NOD-derived MSC C57BL/6-derived MSC BALC/c-derived MSC adenovirus K2 transfection system |
| title | K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells |
| title_full | K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells |
| title_fullStr | K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells |
| title_full_unstemmed | K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells |
| title_short | K2 Transfection System Boosts the Adenoviral Transduction of Murine Mesenchymal Stromal Cells |
| title_sort | k2 transfection system boosts the adenoviral transduction of murine mesenchymal stromal cells |
| topic | mesenchymal stromal cells NOD-derived MSC C57BL/6-derived MSC BALC/c-derived MSC adenovirus K2 transfection system |
| url | https://www.mdpi.com/1422-0067/22/2/598 |
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