Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy
Mesenchymal stem cells serve as the body’s reservoir for healing and tissue regeneration. In cases of severe tissue trauma where there is also a need for tissue organization, a scaffold may be of use to support the cells in the damaged tissue. Such a scaffold should be composed of a materi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-03-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/10/3/458 |
| _version_ | 1818450898172510208 |
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| author | Leslie Pomeraniec Dafna Benayahu |
| author_facet | Leslie Pomeraniec Dafna Benayahu |
| author_sort | Leslie Pomeraniec |
| collection | DOAJ |
| description | Mesenchymal stem cells serve as the body’s reservoir for healing and tissue regeneration. In cases of severe tissue trauma where there is also a need for tissue organization, a scaffold may be of use to support the cells in the damaged tissue. Such a scaffold should be composed of a material that can biomimic the mechanical and biological properties of the target tissues in order to support autologous cell-adhesion, their proliferation, and differentiation. In this study, we developed and assayed a new biocomposite made of unique collagen fibers and alginate hydrogel that was assessed for the ability to support mesenchymal cell-proliferation and differentiation. Analysis over 11 weeks in vitro demonstrated that the scaffold was biocompatible and supports the cells viability and differentiation to produce tissue-like structures or become adipocyte under differentiation medium. When the biocomposite was enriched with nano particles (NPs), mesenchymal cells grew well after uptake of fluorescein isothiocyanate (FITC) labeled NPs, maintained their viability, migrated through the biocomposite, reached, and adhered to the tissue culture dish. These promising findings revealed that the scaffold supports the growth and differentiation of mesenchymal cells that demonstrate their full physiological function with no sign of material toxicity. The cells’ functionality performance indicates and suggests that the scaffold is suitable to be developed as a new medical device that has the potential to support regeneration and the production of functional tissue. |
| first_indexed | 2024-12-14T20:58:37Z |
| format | Article |
| id | doaj.art-f8d4377d2158493e96edbe8712ce1835 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-14T20:58:37Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-f8d4377d2158493e96edbe8712ce18352022-12-21T22:47:38ZengMDPI AGBiomolecules2218-273X2020-03-0110345810.3390/biom10030458biom10030458Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration TherapyLeslie Pomeraniec0Dafna Benayahu1Department of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, IsraelDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, IsraelMesenchymal stem cells serve as the body’s reservoir for healing and tissue regeneration. In cases of severe tissue trauma where there is also a need for tissue organization, a scaffold may be of use to support the cells in the damaged tissue. Such a scaffold should be composed of a material that can biomimic the mechanical and biological properties of the target tissues in order to support autologous cell-adhesion, their proliferation, and differentiation. In this study, we developed and assayed a new biocomposite made of unique collagen fibers and alginate hydrogel that was assessed for the ability to support mesenchymal cell-proliferation and differentiation. Analysis over 11 weeks in vitro demonstrated that the scaffold was biocompatible and supports the cells viability and differentiation to produce tissue-like structures or become adipocyte under differentiation medium. When the biocomposite was enriched with nano particles (NPs), mesenchymal cells grew well after uptake of fluorescein isothiocyanate (FITC) labeled NPs, maintained their viability, migrated through the biocomposite, reached, and adhered to the tissue culture dish. These promising findings revealed that the scaffold supports the growth and differentiation of mesenchymal cells that demonstrate their full physiological function with no sign of material toxicity. The cells’ functionality performance indicates and suggests that the scaffold is suitable to be developed as a new medical device that has the potential to support regeneration and the production of functional tissue.https://www.mdpi.com/2218-273X/10/3/458mesenchymal cellsscaffoldbiocompositetissue regeneration |
| spellingShingle | Leslie Pomeraniec Dafna Benayahu Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy Biomolecules mesenchymal cells scaffold biocomposite tissue regeneration |
| title | Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy |
| title_full | Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy |
| title_fullStr | Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy |
| title_full_unstemmed | Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy |
| title_short | Mesenchymal Cell Growth and Differentiation on a New Biocomposite Material: A Promising Model for Regeneration Therapy |
| title_sort | mesenchymal cell growth and differentiation on a new biocomposite material a promising model for regeneration therapy |
| topic | mesenchymal cells scaffold biocomposite tissue regeneration |
| url | https://www.mdpi.com/2218-273X/10/3/458 |
| work_keys_str_mv | AT lesliepomeraniec mesenchymalcellgrowthanddifferentiationonanewbiocompositematerialapromisingmodelforregenerationtherapy AT dafnabenayahu mesenchymalcellgrowthanddifferentiationonanewbiocompositematerialapromisingmodelforregenerationtherapy |