Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh
Since Streptococcus mutans appears to be the most common cause of dental caries, appropriate laboratory media is necessary for the proper detection and management of this bacterium. The aim of this study was to evaluate the mitis salivarius agar (MSA) compared to conventional blood agar media (BAM)...
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| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2022-08-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=1239 |
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| author | Jannatul Ferdose Md. Shah Alam Anika Tasnim Md. Rayhan Chowdhury Md. Mim Obaidullah Md. Nurul Matin Md. Abu Sayem |
| author_facet | Jannatul Ferdose Md. Shah Alam Anika Tasnim Md. Rayhan Chowdhury Md. Mim Obaidullah Md. Nurul Matin Md. Abu Sayem |
| author_sort | Jannatul Ferdose |
| collection | DOAJ |
| description | Since Streptococcus mutans appears to be the most common cause of dental caries, appropriate laboratory media is necessary for the proper detection and management of this bacterium. The aim of this study was to evaluate the mitis salivarius agar (MSA) compared to conventional blood agar media (BAM) for detection of bacterium. This study was conducted in the Department of Microbiology of the Rajshahi Medical College, Rajshahi, Bangladesh from April 2017 to December 2017. The sample, dental swab was taken from 200 children, aged between 6-18 years who underwent dental caries and residing in Rajshahi district. All specimens were cultured to identify and compare the morphologic characteristics of its colonies both in MSA and BAM. In this study, antimicrobial susceptibility testing was also performed. This prospective observational study was conducted through regular and continuous monitoring of the results. Out of 200 specimens, the growth rate was 82%. Higher growth was observed in MSA (39.5%) than BAM (24.1%). Of them, we found 53.1% multi-drug resistant mutans. The most resistance was to Penicillin G (100%) followed by Azithromycin (95.3%). The study findings would help to increase the detection of mutans and its pattern for proper treatment towards the improvement of children's dental health in Bangladesh. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 283-291] |
| first_indexed | 2024-12-12T04:23:03Z |
| format | Article |
| id | doaj.art-f8d6947f089c47bb899f687d2dfbb149 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-12T04:23:03Z |
| publishDate | 2022-08-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-f8d6947f089c47bb899f687d2dfbb1492022-12-22T00:38:17ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602022-08-015228329110.5455/jabet.2022.d1151239Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in BangladeshJannatul Ferdose0Md. Shah Alam1Anika Tasnim2Md. Rayhan Chowdhury3Md. Mim Obaidullah4Md. Nurul Matin5Md. Abu Sayem6Department of Pathology and Microbiology, National Institute of Diseases of 8 the Chest and Hospital, Mohakhali, Dhaka, Bangladesh. Department of Microbiology, Rajshahi Medical College, Rajshahi, 10 Bangladesh. Department of Microbiology, Parkview Medical College, Sylhet, Bangladesh. Molecular Genetics Laboratory Department of Biochemistry and Cell Biology, Bangladesh University of Health Sciences BUHS, Mirpur, Dhaka 1216, Bangladesh Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh. Institute of Biological Sciences, University of Rajshahi, Bangladesh.Since Streptococcus mutans appears to be the most common cause of dental caries, appropriate laboratory media is necessary for the proper detection and management of this bacterium. The aim of this study was to evaluate the mitis salivarius agar (MSA) compared to conventional blood agar media (BAM) for detection of bacterium. This study was conducted in the Department of Microbiology of the Rajshahi Medical College, Rajshahi, Bangladesh from April 2017 to December 2017. The sample, dental swab was taken from 200 children, aged between 6-18 years who underwent dental caries and residing in Rajshahi district. All specimens were cultured to identify and compare the morphologic characteristics of its colonies both in MSA and BAM. In this study, antimicrobial susceptibility testing was also performed. This prospective observational study was conducted through regular and continuous monitoring of the results. Out of 200 specimens, the growth rate was 82%. Higher growth was observed in MSA (39.5%) than BAM (24.1%). Of them, we found 53.1% multi-drug resistant mutans. The most resistance was to Penicillin G (100%) followed by Azithromycin (95.3%). The study findings would help to increase the detection of mutans and its pattern for proper treatment towards the improvement of children's dental health in Bangladesh. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 283-291]http://www.ejmanager.com/fulltextpdf.php?mno=1239streptococcus mutansmitis salivariusblood agarantimicrobial susceptibility |
| spellingShingle | Jannatul Ferdose Md. Shah Alam Anika Tasnim Md. Rayhan Chowdhury Md. Mim Obaidullah Md. Nurul Matin Md. Abu Sayem Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh Journal of Advanced Biotechnology and Experimental Therapeutics streptococcus mutans mitis salivarius blood agar antimicrobial susceptibility |
| title | Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh |
| title_full | Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh |
| title_fullStr | Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh |
| title_full_unstemmed | Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh |
| title_short | Evaluation of selective mitis salivarius agar for the isolation of Streptococcus mutans and its resistance pattern in Bangladesh |
| title_sort | evaluation of selective mitis salivarius agar for the isolation of streptococcus mutans and its resistance pattern in bangladesh |
| topic | streptococcus mutans mitis salivarius blood agar antimicrobial susceptibility |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=1239 |
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