A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella phaffii

A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.

Bibliographic Details
Main Authors: Yoichiro Ito, Misa Ishigami, Goro Terai, Yasuyuki Nakamura, Noriko Hashiba, Teruyuki Nishi, Hikaru Nakazawa, Tomohisa Hasunuma, Kiyoshi Asai, Mitsuo Umetsu, Jun Ishii, Akihiko Kondo
Format: Article
Language:English
Published: Nature Portfolio 2022-06-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-03475-w
Description
Summary:A high-throughput workflow involving a streamlined genome-wide screen improves protein production in the non-conventional yeast, Komagataella phaffii, following adaptive laboratory evolution.
ISSN:2399-3642