Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis.
Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacil...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-09-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC5023120?pdf=render |
| _version_ | 1818311223730503680 |
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| author | Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka |
| author_facet | Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka |
| author_sort | Cláudia Gomes |
| collection | DOAJ |
| description | Bartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas.Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit α (SCS-α) and succinyl-CoA synthetase subunit β (SCS-β). On Western blot both Pap31 and SCS-α interacted with IgM, while GroEL and SCS-β interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-α) to 97.2% (IgM against Pap31).RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers. |
| first_indexed | 2024-12-13T07:58:33Z |
| format | Article |
| id | doaj.art-f8d7c9d7013045cf849feb2eb155f8e7 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-13T07:58:33Z |
| publishDate | 2016-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-f8d7c9d7013045cf849feb2eb155f8e72022-12-21T23:54:30ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-09-01109e000498910.1371/journal.pntd.0004989Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis.Cláudia GomesNoemí PalmaMaria J PonsAriel Magallón-TejadaIsabel SandovalCarmen Tinco-ValdezCarlos GutarraJuana Del Valle-MendozaJoaquim RuizMayumi MatsuokaBartonella bacilliformis is the causative agent of Carrion's disease, a neglected illness with mortality rates of 40-85% in the absence of treatment. The lack of a diagnostic technique to overcome misdiagnosis and treat asymptomatic carriers is of note. This study aimed to identify new B. bacilliformis antigenic candidates that could lead to a new diagnostic tool able to be implemented in endemic rural areas.Blood (n = 198) and serum (n = 177) samples were collected in northern Peru. Clinical data were recorded. Specific 16S rRNA amplification by RT-PCR, IFA and ELISA for IgM/IgG with whole cells as antigens was done. Western blot analysis and N-terminal amino acid sequencing detected seroreactive proteins. ELISAs for IgM/IgG for the antigenic candidates were performed. Of the population 33.3% reported at least one symptom compatible with Carrion's disease; 25.4% (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR) of samples were positive. Four proteins were considered potential antigenic candidates, including two new antigenic candidates, succinyl-CoA synthetase subunit α (SCS-α) and succinyl-CoA synthetase subunit β (SCS-β). On Western blot both Pap31 and SCS-α interacted with IgM, while GroEL and SCS-β interacted with IgG. The presence of specific antibodies against the antigenic candidates varied from 34.5% (IgG against SCS-α) to 97.2% (IgM against Pap31).RT-PCR and the high levels of positivity for specific ELISAs demonstrate high levels of B. bacilliformis exposure and asymptomatic carriers among inhabitants. The new antigens identified might be used as a new rapid diagnostic tool to diagnose acute Carrion's disease and identify asymptomatic carriers.http://europepmc.org/articles/PMC5023120?pdf=render |
| spellingShingle | Cláudia Gomes Noemí Palma Maria J Pons Ariel Magallón-Tejada Isabel Sandoval Carmen Tinco-Valdez Carlos Gutarra Juana Del Valle-Mendoza Joaquim Ruiz Mayumi Matsuoka Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. PLoS Neglected Tropical Diseases |
| title | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
| title_full | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
| title_fullStr | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
| title_full_unstemmed | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
| title_short | Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis. |
| title_sort | succinyl coa synthetase new antigen candidate of bartonella bacilliformis |
| url | http://europepmc.org/articles/PMC5023120?pdf=render |
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