Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i>
In recent years, the bacterial blight of cassava has caused substantial economic losses to the Chinese cassava industry. Chemical control methods have become the primary approach to control this disease; however, their widespread usage and harmful residues have raised concerns about environmental po...
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MDPI AG
2023-09-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/12/9/1231 |
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| author | Yating Feng Yijie Zhang Obaid Ullah Shah Kai Luo Yinhua Chen |
| author_facet | Yating Feng Yijie Zhang Obaid Ullah Shah Kai Luo Yinhua Chen |
| author_sort | Yating Feng |
| collection | DOAJ |
| description | In recent years, the bacterial blight of cassava has caused substantial economic losses to the Chinese cassava industry. Chemical control methods have become the primary approach to control this disease; however, their widespread usage and harmful residues have raised concerns about environmental pollution. In order to avoid this, it is urgent to seek a green ecological method to prevent and control it. Biological control through the utilization of microorganisms not only effectively inhibits the disease, but also gives consideration to environmental friendliness. Therefore, investigating an endophytic biological control method for cassava bacterial blight is of great importance. In this study, cassava leaf tissues were used as test specimens in order to isolate endophytic bacteria by using dilution and separation methods. <i>Bacillus</i> ME9, derived from cassava endophytic bacteria, exhibits good antagonism against a diverse range of pathogens, including <i>Xpm</i>11. Its genome consists of a series of genes encoding antibacterial lipopeptides, which may be directly related to its antibacterial capabilities. Furthermore, inoculation resulted in a substantial change in the diversity of the endophytic bacterial community, characterized by improved diversity, and displayed an obvious inhibition of pathogenic bacterial growth, demonstrating successful colonization within plants. The results laid a foundation and provided theoretical support for the development and utilization of cassava endophytic bacterial diversity and endogenous disease control strategies. |
| first_indexed | 2024-03-10T23:01:59Z |
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| id | doaj.art-f8d9537d89444832b32350aa30f344ff |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T23:01:59Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
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| spelling | doaj.art-f8d9537d89444832b32350aa30f344ff2023-11-19T09:38:51ZengMDPI AGBiology2079-77372023-09-01129123110.3390/biology12091231Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i>Yating Feng0Yijie Zhang1Obaid Ullah Shah2Kai Luo3Yinhua Chen4Sanya Nanfan Research Institute, Hainan University, Sanya 572025, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572025, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572025, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572025, ChinaSanya Nanfan Research Institute, Hainan University, Sanya 572025, ChinaIn recent years, the bacterial blight of cassava has caused substantial economic losses to the Chinese cassava industry. Chemical control methods have become the primary approach to control this disease; however, their widespread usage and harmful residues have raised concerns about environmental pollution. In order to avoid this, it is urgent to seek a green ecological method to prevent and control it. Biological control through the utilization of microorganisms not only effectively inhibits the disease, but also gives consideration to environmental friendliness. Therefore, investigating an endophytic biological control method for cassava bacterial blight is of great importance. In this study, cassava leaf tissues were used as test specimens in order to isolate endophytic bacteria by using dilution and separation methods. <i>Bacillus</i> ME9, derived from cassava endophytic bacteria, exhibits good antagonism against a diverse range of pathogens, including <i>Xpm</i>11. Its genome consists of a series of genes encoding antibacterial lipopeptides, which may be directly related to its antibacterial capabilities. Furthermore, inoculation resulted in a substantial change in the diversity of the endophytic bacterial community, characterized by improved diversity, and displayed an obvious inhibition of pathogenic bacterial growth, demonstrating successful colonization within plants. The results laid a foundation and provided theoretical support for the development and utilization of cassava endophytic bacterial diversity and endogenous disease control strategies.https://www.mdpi.com/2079-7737/12/9/1231cassavaendophyte<i>Bacillus subtilis</i>bacterial blightisolationidentification |
| spellingShingle | Yating Feng Yijie Zhang Obaid Ullah Shah Kai Luo Yinhua Chen Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> Biology cassava endophyte <i>Bacillus subtilis</i> bacterial blight isolation identification |
| title | Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> |
| title_full | Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> |
| title_fullStr | Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> |
| title_full_unstemmed | Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> |
| title_short | Isolation and Identification of Endophytic Bacteria <i>Bacillus</i> sp. ME9 That Exhibits Biocontrol Activity against <i>Xanthomonas phaseoli</i> pv. <i>manihotis</i> |
| title_sort | isolation and identification of endophytic bacteria i bacillus i sp me9 that exhibits biocontrol activity against i xanthomonas phaseoli i pv i manihotis i |
| topic | cassava endophyte <i>Bacillus subtilis</i> bacterial blight isolation identification |
| url | https://www.mdpi.com/2079-7737/12/9/1231 |
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