Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows
ABSTRACT: Mastitis is one of the most frequent and costly diseases affecting dairy cattle. Natural antibodies (immunoglobulins) and cyclophilin A (CyPA), the most abundant member of the family of peptidyl prolyl cis/trans isomerases, in milk may serve as indicators of mastitis resistance in dairy ca...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223007105 |
| _version_ | 1797291872074334208 |
|---|---|
| author | Yoshinobu Uemoto Teppei Katsura Yuma Endo Koutaro Tanaka Tao Zhuang Megumi Urakawa Toshimi Baba Gaku Yoshida Haifei Wang Haruki Kitazawa Hitoshi Shirakawa Takehiko Nakamura Tomonori Nochi Hisashi Aso |
| author_facet | Yoshinobu Uemoto Teppei Katsura Yuma Endo Koutaro Tanaka Tao Zhuang Megumi Urakawa Toshimi Baba Gaku Yoshida Haifei Wang Haruki Kitazawa Hitoshi Shirakawa Takehiko Nakamura Tomonori Nochi Hisashi Aso |
| author_sort | Yoshinobu Uemoto |
| collection | DOAJ |
| description | ABSTRACT: Mastitis is one of the most frequent and costly diseases affecting dairy cattle. Natural antibodies (immunoglobulins) and cyclophilin A (CyPA), the most abundant member of the family of peptidyl prolyl cis/trans isomerases, in milk may serve as indicators of mastitis resistance in dairy cattle. However, genetic information for CyPA is not available, and knowledge on the genetic and nongenetic relationships between these immune-related traits and somatic cell score (SCS) and milk yield in dairy cattle is sparse. Therefore, we aimed to comprehensively evaluate whether immune-related traits consisting of 5 Ig classes (IgG, IgG1, IgG2, IgA, and IgM) and CyPA in the test-day milk of Holstein cows can be used as genetic indicators of mastitis resistance by evaluating the genetic and nongenetic relationships with SCS in milk. The nongenetic factors affecting immune-related traits and the effects of these traits on SCS were evaluated. Furthermore, the genetic parameters of immune-related traits according to health status and genetic relationships under different SCS environments were estimated. All immune-related traits were significantly associated with SCS and directly proportional. Additionally, evaluation using a classification tree revealed that IgA, IgG2, and IgG were associated with SCS levels. Genetic factor analyses indicated that heritability estimates were low for CyPA (0.08) but moderate for IgG (0.37), IgA (0.44), and IgM (0.44), with positive genetic correlations among Ig (0.25–0.96). We also evaluated the differences in milk yield and SCS of cows between the low and high groups according to their sires' estimated breeding value for immune-related traits. In the high group, IgA had a significantly lower SCS in milk at 7 to 30 d compared with that in the low group. Furthermore, the Ig in milk had high positive genetic correlations between healthy and infected conditions (0.82–0.99), suggesting that Ig in milk under healthy conditions could interact with those under infected conditions, owing to the genetic ability based on the level of Ig in milk. Thus, Ig in milk are potential indicators for the genetic selection of mastitis resistance. However, because only the relationship between immune-related traits and SCS was investigated in this study, further study on the relationship between clinical mastitis and Ig in milk is needed before Ig can be used as an indicator of mastitis resistance. |
| first_indexed | 2024-03-07T19:44:01Z |
| format | Article |
| id | doaj.art-f8dd09a00da6494fa6a007ae5a7058e7 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-07T19:44:01Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-f8dd09a00da6494fa6a007ae5a7058e72024-02-29T05:16:59ZengElsevierJournal of Dairy Science0022-03022024-03-01107315771591Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cowsYoshinobu Uemoto0Teppei Katsura1Yuma Endo2Koutaro Tanaka3Tao Zhuang4Megumi Urakawa5Toshimi Baba6Gaku Yoshida7Haifei Wang8Haruki Kitazawa9Hitoshi Shirakawa10Takehiko Nakamura11Tomonori Nochi12Hisashi Aso13Animal Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan; Corresponding authorsAnimal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanAnimal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanAnimal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanHolstein Cattle Association of Japan, Hokkaido Branch, Sapporo, Hokkaido 001-8555, JapanShihoro Agricultural Cooperative, Kato District, Hokkaido 080-1219, JapanKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan; Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan; Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanAnimal Functional Morphology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan; Laboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, JapanLaboratory of Animal Health Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan; The Cattle Museum, Oshu, Iwate 029-4205, Japan; Corresponding authorsABSTRACT: Mastitis is one of the most frequent and costly diseases affecting dairy cattle. Natural antibodies (immunoglobulins) and cyclophilin A (CyPA), the most abundant member of the family of peptidyl prolyl cis/trans isomerases, in milk may serve as indicators of mastitis resistance in dairy cattle. However, genetic information for CyPA is not available, and knowledge on the genetic and nongenetic relationships between these immune-related traits and somatic cell score (SCS) and milk yield in dairy cattle is sparse. Therefore, we aimed to comprehensively evaluate whether immune-related traits consisting of 5 Ig classes (IgG, IgG1, IgG2, IgA, and IgM) and CyPA in the test-day milk of Holstein cows can be used as genetic indicators of mastitis resistance by evaluating the genetic and nongenetic relationships with SCS in milk. The nongenetic factors affecting immune-related traits and the effects of these traits on SCS were evaluated. Furthermore, the genetic parameters of immune-related traits according to health status and genetic relationships under different SCS environments were estimated. All immune-related traits were significantly associated with SCS and directly proportional. Additionally, evaluation using a classification tree revealed that IgA, IgG2, and IgG were associated with SCS levels. Genetic factor analyses indicated that heritability estimates were low for CyPA (0.08) but moderate for IgG (0.37), IgA (0.44), and IgM (0.44), with positive genetic correlations among Ig (0.25–0.96). We also evaluated the differences in milk yield and SCS of cows between the low and high groups according to their sires' estimated breeding value for immune-related traits. In the high group, IgA had a significantly lower SCS in milk at 7 to 30 d compared with that in the low group. Furthermore, the Ig in milk had high positive genetic correlations between healthy and infected conditions (0.82–0.99), suggesting that Ig in milk under healthy conditions could interact with those under infected conditions, owing to the genetic ability based on the level of Ig in milk. Thus, Ig in milk are potential indicators for the genetic selection of mastitis resistance. However, because only the relationship between immune-related traits and SCS was investigated in this study, further study on the relationship between clinical mastitis and Ig in milk is needed before Ig can be used as an indicator of mastitis resistance.http://www.sciencedirect.com/science/article/pii/S0022030223007105cyclophilin Agenetic parameterimmunoglobulinssomatic cell score |
| spellingShingle | Yoshinobu Uemoto Teppei Katsura Yuma Endo Koutaro Tanaka Tao Zhuang Megumi Urakawa Toshimi Baba Gaku Yoshida Haifei Wang Haruki Kitazawa Hitoshi Shirakawa Takehiko Nakamura Tomonori Nochi Hisashi Aso Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows Journal of Dairy Science cyclophilin A genetic parameter immunoglobulins somatic cell score |
| title | Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows |
| title_full | Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows |
| title_fullStr | Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows |
| title_full_unstemmed | Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows |
| title_short | Genetic aspects of immunoglobulins and cyclophilin A in milk as potential indicators of mastitis resistance in Holstein cows |
| title_sort | genetic aspects of immunoglobulins and cyclophilin a in milk as potential indicators of mastitis resistance in holstein cows |
| topic | cyclophilin A genetic parameter immunoglobulins somatic cell score |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223007105 |
| work_keys_str_mv | AT yoshinobuuemoto geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT teppeikatsura geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT yumaendo geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT koutarotanaka geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT taozhuang geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT megumiurakawa geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT toshimibaba geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT gakuyoshida geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT haifeiwang geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT harukikitazawa geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT hitoshishirakawa geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT takehikonakamura geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT tomonorinochi geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows AT hisashiaso geneticaspectsofimmunoglobulinsandcyclophilinainmilkaspotentialindicatorsofmastitisresistanceinholsteincows |