Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine
Introduction: Growth factors are crucial bioactive molecules in vitro and in vivo. Among them, basic fibroblast growth factor (bFGF) has been used widely for various applications such as cell culture and regenerative medicine. However, bFGF has extremely poor stability in aqueous solution; thus, it...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235232042030078X |
| _version_ | 1818611342065532928 |
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| author | Yasuhiro Ikegami Hideyuki Mizumachi Kozue Yoshida Hiroyuki Ijima |
| author_facet | Yasuhiro Ikegami Hideyuki Mizumachi Kozue Yoshida Hiroyuki Ijima |
| author_sort | Yasuhiro Ikegami |
| collection | DOAJ |
| description | Introduction: Growth factors are crucial bioactive molecules in vitro and in vivo. Among them, basic fibroblast growth factor (bFGF) has been used widely for various applications such as cell culture and regenerative medicine. However, bFGF has extremely poor stability in aqueous solution; thus, it is difficult to maintain its high local concentration. Heparin-conjugated materials have been studied recently as promising scaffold-immobilizing growth factors for biological and medical applications. The previous studies have focused on the local concentration maintenance and sustained release of the growth factors from the scaffold. Methods: In this paper, we focused on the biological stability of bFGF immobilized on the heparin-conjugated collagen (hep-col) scaffold. The stability of the immobilized bFGF was quantitatively evaluated at physiological temperature (37 °C) using cell culture and ELISA. Results: The immobilized bFGF had twice higher stability than the bFGF solution. Furthermore, the hep-col scaffold was able to immobilize not only bFGF but also other growth factors (i.e., vascular endothelial growth factor and hepatocyte growth factor) at high efficiency. Conclusions: The hep-col scaffold can localize several kinds of growth factors as well as stabilize bFGF under physiological temperature and is a promising potent scaffold for regenerative medicine. |
| first_indexed | 2024-12-16T15:28:48Z |
| format | Article |
| id | doaj.art-f8deba3ed76342fa8b15973664839fef |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-16T15:28:48Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-f8deba3ed76342fa8b15973664839fef2022-12-21T22:26:25ZengElsevierRegenerative Therapy2352-32042020-12-0115236242Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicineYasuhiro Ikegami0Hideyuki Mizumachi1Kozue Yoshida2Hiroyuki Ijima3Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, JapanDepartment of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, JapanDepartment of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, JapanCorresponding author. Fax: +81-92-802-2748.; Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, JapanIntroduction: Growth factors are crucial bioactive molecules in vitro and in vivo. Among them, basic fibroblast growth factor (bFGF) has been used widely for various applications such as cell culture and regenerative medicine. However, bFGF has extremely poor stability in aqueous solution; thus, it is difficult to maintain its high local concentration. Heparin-conjugated materials have been studied recently as promising scaffold-immobilizing growth factors for biological and medical applications. The previous studies have focused on the local concentration maintenance and sustained release of the growth factors from the scaffold. Methods: In this paper, we focused on the biological stability of bFGF immobilized on the heparin-conjugated collagen (hep-col) scaffold. The stability of the immobilized bFGF was quantitatively evaluated at physiological temperature (37 °C) using cell culture and ELISA. Results: The immobilized bFGF had twice higher stability than the bFGF solution. Furthermore, the hep-col scaffold was able to immobilize not only bFGF but also other growth factors (i.e., vascular endothelial growth factor and hepatocyte growth factor) at high efficiency. Conclusions: The hep-col scaffold can localize several kinds of growth factors as well as stabilize bFGF under physiological temperature and is a promising potent scaffold for regenerative medicine.http://www.sciencedirect.com/science/article/pii/S235232042030078XHeparin-conjugated collagenBasic fibroblast growth factorGrowth factor stabilizationBioactive growth factor-quantification |
| spellingShingle | Yasuhiro Ikegami Hideyuki Mizumachi Kozue Yoshida Hiroyuki Ijima Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine Regenerative Therapy Heparin-conjugated collagen Basic fibroblast growth factor Growth factor stabilization Bioactive growth factor-quantification |
| title | Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine |
| title_full | Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine |
| title_fullStr | Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine |
| title_full_unstemmed | Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine |
| title_short | Heparin-conjugated collagen as a potent growth factor-localizing and stabilizing scaffold for regenerative medicine |
| title_sort | heparin conjugated collagen as a potent growth factor localizing and stabilizing scaffold for regenerative medicine |
| topic | Heparin-conjugated collagen Basic fibroblast growth factor Growth factor stabilization Bioactive growth factor-quantification |
| url | http://www.sciencedirect.com/science/article/pii/S235232042030078X |
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