Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples
Preterm birth (PTB) affects one in six Black babies in the United States. Epigenetics is believed to play a role in PTB; however, only a limited number of epigenetic studies of PTB have been reported, most of which have focused on cord blood DNA methylation (DNAm) and/or were conducted in white popu...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-02-01
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| Series: | Epigenetics |
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| Online Access: | http://dx.doi.org/10.1080/15592294.2017.1287654 |
| _version_ | 1797678996347944960 |
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| author | Xiumei Hong Ben Sherwood Christine Ladd-Acosta Shouneng Peng Hongkai Ji Ke Hao Irina Burd Tami R Bartell Guoying Wang Hui-Ju Tsai Xin Liu Yuelong Ji Anastacia Wahl Deanna Caruso Aviva Lee-Parritz Barry Zuckerman Xiaobin Wang |
| author_facet | Xiumei Hong Ben Sherwood Christine Ladd-Acosta Shouneng Peng Hongkai Ji Ke Hao Irina Burd Tami R Bartell Guoying Wang Hui-Ju Tsai Xin Liu Yuelong Ji Anastacia Wahl Deanna Caruso Aviva Lee-Parritz Barry Zuckerman Xiaobin Wang |
| author_sort | Xiumei Hong |
| collection | DOAJ |
| description | Preterm birth (PTB) affects one in six Black babies in the United States. Epigenetics is believed to play a role in PTB; however, only a limited number of epigenetic studies of PTB have been reported, most of which have focused on cord blood DNA methylation (DNAm) and/or were conducted in white populations. Here we conducted, by far, the largest epigenome-wide DNAm analysis in 300 Black women who delivered early spontaneous preterm (sPTB, n = 150) or full-term babies (n = 150) and replicated the findings in an independent set of Black mother-newborn pairs from the Boston Birth Cohort. DNAm in maternal blood and/or cord blood was measured using the Illumina HumanMethylation450 BeadChip. We identified 45 DNAm loci in maternal blood associated with early sPTB, with a false discovery rate (FDR) <5%. Replication analyses confirmed sPTB associations for cg03915055 and cg06804705, located in the promoter regions of the CYTIP and LINC00114 genes, respectively. Both loci had comparable associations with early sPTB and early medically-indicated PTB, but attenuated associations with late sPTB. These associations could not be explained by cell composition, gestational complications, and/or nearby maternal genetic variants. Analyses in the newborns of the 110 Black women showed that cord blood methylation levels at both loci had no associations with PTB. The findings from this study underscore the role of maternal DNAm in PTB risk, and provide a set of maternal loci that may serve as biomarkers for PTB. Longitudinal studies are needed to clarify temporal relationships between maternal DNAm and PTB risk. |
| first_indexed | 2024-03-11T23:07:59Z |
| format | Article |
| id | doaj.art-f8deda086ce7412aa3189ef3f775d6c6 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:59Z |
| publishDate | 2018-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-f8deda086ce7412aa3189ef3f775d6c62023-09-21T12:43:12ZengTaylor & Francis GroupEpigenetics1559-22941559-23082018-02-0113216317210.1080/15592294.2017.12876541287654Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samplesXiumei Hong0Ben Sherwood1Christine Ladd-Acosta2Shouneng Peng3Hongkai Ji4Ke Hao5Irina Burd6Tami R Bartell7Guoying Wang8Hui-Ju Tsai9Xin Liu10Yuelong Ji11Anastacia Wahl12Deanna Caruso13Aviva Lee-Parritz14Barry Zuckerman15Xiaobin Wang16Family and Reproductive HealthJohns Hopkins University Bloomberg School of Public Health, BaltimoreJohns Hopkins Bloomberg School of Public HealthDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount SinaiJohns Hopkins University Bloomberg School of Public Health, BaltimoreDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount SinaiJohns Hopkins University School of MedicineStanley Manne Children's Research InstituteFamily and Reproductive HealthInstitute of Population Health SciencesNorthwestern UniversityFamily and Reproductive HealthBoston University School of Medicine and Boston Medical CenterFamily and Reproductive HealthBoston University School of MedicineBoston University School of Medicine and Boston Medical CenterFamily and Reproductive HealthPreterm birth (PTB) affects one in six Black babies in the United States. Epigenetics is believed to play a role in PTB; however, only a limited number of epigenetic studies of PTB have been reported, most of which have focused on cord blood DNA methylation (DNAm) and/or were conducted in white populations. Here we conducted, by far, the largest epigenome-wide DNAm analysis in 300 Black women who delivered early spontaneous preterm (sPTB, n = 150) or full-term babies (n = 150) and replicated the findings in an independent set of Black mother-newborn pairs from the Boston Birth Cohort. DNAm in maternal blood and/or cord blood was measured using the Illumina HumanMethylation450 BeadChip. We identified 45 DNAm loci in maternal blood associated with early sPTB, with a false discovery rate (FDR) <5%. Replication analyses confirmed sPTB associations for cg03915055 and cg06804705, located in the promoter regions of the CYTIP and LINC00114 genes, respectively. Both loci had comparable associations with early sPTB and early medically-indicated PTB, but attenuated associations with late sPTB. These associations could not be explained by cell composition, gestational complications, and/or nearby maternal genetic variants. Analyses in the newborns of the 110 Black women showed that cord blood methylation levels at both loci had no associations with PTB. The findings from this study underscore the role of maternal DNAm in PTB risk, and provide a set of maternal loci that may serve as biomarkers for PTB. Longitudinal studies are needed to clarify temporal relationships between maternal DNAm and PTB risk.http://dx.doi.org/10.1080/15592294.2017.1287654dna methylationepigenome-wide associationsmaternal bloodspontaneous preterm birth |
| spellingShingle | Xiumei Hong Ben Sherwood Christine Ladd-Acosta Shouneng Peng Hongkai Ji Ke Hao Irina Burd Tami R Bartell Guoying Wang Hui-Ju Tsai Xin Liu Yuelong Ji Anastacia Wahl Deanna Caruso Aviva Lee-Parritz Barry Zuckerman Xiaobin Wang Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples Epigenetics dna methylation epigenome-wide associations maternal blood spontaneous preterm birth |
| title | Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples |
| title_full | Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples |
| title_fullStr | Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples |
| title_full_unstemmed | Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples |
| title_short | Genome-wide DNA methylation associations with spontaneous preterm birth in US blacks: findings in maternal and cord blood samples |
| title_sort | genome wide dna methylation associations with spontaneous preterm birth in us blacks findings in maternal and cord blood samples |
| topic | dna methylation epigenome-wide associations maternal blood spontaneous preterm birth |
| url | http://dx.doi.org/10.1080/15592294.2017.1287654 |
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