Cell-binding microarray application in diagnosis of hairy cell leukemia
We describe an application of a cell-binding microarray – to parallel study of morphology, tartrate-resistant acid phosphatase activity and detection of surface markers on peripheral blood lymphocytes of 90 atients with suspected hairy cell leukemia (HCL). We have formulated the microarray-based di...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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ABV-press
2015-06-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/137 |
| _version_ | 1797855579023081472 |
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| author | A. N. Khvastunova L. S. Al-Radi N. M. Kapranov O. S. Fedyanina L. A. Gorgidze S. A. Lugovskaya E. V. Naumova U. L. Dzhulakyan A. V. Filatov F. I. Ataullakhanov S. A. Kuznetsova |
| author_facet | A. N. Khvastunova L. S. Al-Radi N. M. Kapranov O. S. Fedyanina L. A. Gorgidze S. A. Lugovskaya E. V. Naumova U. L. Dzhulakyan A. V. Filatov F. I. Ataullakhanov S. A. Kuznetsova |
| author_sort | A. N. Khvastunova |
| collection | DOAJ |
| description | We describe an application of a cell-binding microarray – to parallel study of morphology, tartrate-resistant acid phosphatase activity and detection of surface markers on peripheral blood lymphocytes of 90 atients with suspected hairy cell leukemia (HCL). We have formulated the microarray-based diagnostic criteria for hairy cell leukemia, hairy cell leukemia variant (HCLv) and splenic marginal zone lymphoma (SMZL). According to these criteria we have suggested the presence of HCL for 55 patients, HCLv – for 7 patients and SMZL – for 10 patients from the studied cohort. These diagnoses were confirmed by standard diagnostic methods in all cases. These results show that the cellbinding microarray can be used in differential diagnosis of HCL, while the high sensitivity of the microarray permits to detect the leukemic cells in spite of leukopenia and low hairy cell content. |
| first_indexed | 2024-04-09T20:26:07Z |
| format | Article |
| id | doaj.art-f8e0bf2d3987422e9c1b569a2b1646d4 |
| institution | Directory Open Access Journal |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| last_indexed | 2024-04-09T20:26:07Z |
| publishDate | 2015-06-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj.art-f8e0bf2d3987422e9c1b569a2b1646d42023-03-30T20:15:10ZrusABV-pressОнкогематология1818-83462413-40232015-06-01101374510.17650/1818-8346-2015-1-37-45152Cell-binding microarray application in diagnosis of hairy cell leukemiaA. N. Khvastunova0L. S. Al-Radi1N. M. Kapranov2O. S. Fedyanina3L. A. Gorgidze4S. A. Lugovskaya5E. V. Naumova6U. L. Dzhulakyan7A. V. Filatov8F. I. Ataullakhanov9S. A. Kuznetsova10Federal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, Russia Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, RussiaHematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaTheoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaTheoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, RussiaHematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaRussian Medical Academy of Postgraduate Education, Ministry of Health of Russia; 2/1 Barrikadnaya St., Moscow, 125993, RussiaRussian Medical Academy of Postgraduate Education, Ministry of Health of Russia; 2/1 Barrikadnaya St., Moscow, 125993, RussiaHematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaState Research Center “Institute of Immunology”, Federal Medical and Biological Agency, Moscow; 24, Bldg. 2 Kashirskoe Shosse, Moscow, 115478, RussiaFederal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, Russia Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaFederal Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitriy Rogachev, Ministry of Health of Russia; 1 Samory Mashela St., Moscow, 117198, Russia Theoretical Problems Center of Physical and Chemical Pharmacology, Russian Academy of Sciences; 38A, Bldg. 1 Leninskiy Pr., Moscow, 119991, Russia Hematological Research Center, Ministry of Health of Russia; 4a Novo-Zykovskiy Pr-d, Moscow, 125167, RussiaWe describe an application of a cell-binding microarray – to parallel study of morphology, tartrate-resistant acid phosphatase activity and detection of surface markers on peripheral blood lymphocytes of 90 atients with suspected hairy cell leukemia (HCL). We have formulated the microarray-based diagnostic criteria for hairy cell leukemia, hairy cell leukemia variant (HCLv) and splenic marginal zone lymphoma (SMZL). According to these criteria we have suggested the presence of HCL for 55 patients, HCLv – for 7 patients and SMZL – for 10 patients from the studied cohort. These diagnoses were confirmed by standard diagnostic methods in all cases. These results show that the cellbinding microarray can be used in differential diagnosis of HCL, while the high sensitivity of the microarray permits to detect the leukemic cells in spite of leukopenia and low hairy cell content.https://oncohematology.abvpress.ru/ongm/article/view/137anti-cd antibody microarrayhairy cell leukemiahairy cell leukemia variantsplenic marginal zone lymphomacd11ccd25cd103cd123 |
| spellingShingle | A. N. Khvastunova L. S. Al-Radi N. M. Kapranov O. S. Fedyanina L. A. Gorgidze S. A. Lugovskaya E. V. Naumova U. L. Dzhulakyan A. V. Filatov F. I. Ataullakhanov S. A. Kuznetsova Cell-binding microarray application in diagnosis of hairy cell leukemia Онкогематология anti-cd antibody microarray hairy cell leukemia hairy cell leukemia variant splenic marginal zone lymphoma cd11c cd25 cd103 cd123 |
| title | Cell-binding microarray application in diagnosis of hairy cell leukemia |
| title_full | Cell-binding microarray application in diagnosis of hairy cell leukemia |
| title_fullStr | Cell-binding microarray application in diagnosis of hairy cell leukemia |
| title_full_unstemmed | Cell-binding microarray application in diagnosis of hairy cell leukemia |
| title_short | Cell-binding microarray application in diagnosis of hairy cell leukemia |
| title_sort | cell binding microarray application in diagnosis of hairy cell leukemia |
| topic | anti-cd antibody microarray hairy cell leukemia hairy cell leukemia variant splenic marginal zone lymphoma cd11c cd25 cd103 cd123 |
| url | https://oncohematology.abvpress.ru/ongm/article/view/137 |
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