Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors
IntroductionThe Chinese mitten crab (Eriocheir sinensis) is a highly valued freshwater crustacean in China. While the natural shell color of E. sinensis is greenish brown (GH), we found a variety with a brownish-orange shell color (RH). Although RH is more expensive, it exhibits a lower molting freq...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1218152/full |
| _version_ | 1827901700123394048 |
|---|---|
| author | Xiaochen Zhu Yingying Zhao Yingying Zhao Na Sun Changlei Li Qing Jiang Yazhao Zhang Hua Wei Hua Wei Yingdong Li Yingdong Li Qingbiao Hu Qingbiao Hu Xiaodong Li Xiaodong Li Xiaodong Li |
| author_facet | Xiaochen Zhu Yingying Zhao Yingying Zhao Na Sun Changlei Li Qing Jiang Yazhao Zhang Hua Wei Hua Wei Yingdong Li Yingdong Li Qingbiao Hu Qingbiao Hu Xiaodong Li Xiaodong Li Xiaodong Li |
| author_sort | Xiaochen Zhu |
| collection | DOAJ |
| description | IntroductionThe Chinese mitten crab (Eriocheir sinensis) is a highly valued freshwater crustacean in China. While the natural shell color of E. sinensis is greenish brown (GH), we found a variety with a brownish-orange shell color (RH). Although RH is more expensive, it exhibits a lower molting frequency and growth rate compared with GH, which significantly reduces its yield and hinders large-scale farming. The growth and development of animals are closely related to their gut microbiota and gut tissue metabolic profiles.MethodsIn this study, we compared the gut microbiome communities and metabolic profiles of juvenile RH and GH crabs using 16S rRNA gene sequencing and liquid chromatography–mass spectrometry (LC–MS), respectively.ResultsOur findings indicated that the intestinal microbial composition and metabolic characteristics of E. sinensis differed significantly between RH and GH. At the operational taxonomic unit (OTU) level, the α-diversity of the gut microbiota did not differ significantly between RH and GH, while the β-diversity of the RH gut microbiota was higher than that of the GH gut microbiota. At the species level, the richness of unclassified_c_Alphaproteobacteria was significantly higher in the GH group, while the RH group had a significantly higher richness of three low-abundance species, Flavobacteria bacterium BAL38, Paraburkholderia ferrariae, and uncultured_bacterium_g__Legionella. In the current study, 598 gut tissue metabolites were identified, and 159 metabolites were significantly different between GH and RH. The metabolite profile of RH was characteristic of a low level of most amino acids and lipid metabolites and a high level of several pigments compared with that of GH. These metabolites were enriched in 102 KEGG pathways. Four pathways, including (1) Central carbon metabolism in cancer, (2) protein digestion and absorption, (3) alanine, aspartate and glutamate metabolism, and (4) aminoacyl-tRNA biosynthesis, were significantly enriched. The correlation analysis between metabolites and microbiotas indicated that most key differential metabolites were positively correlated with the abundance of Shewanella_sp_MR-7.DiscussionThis research provided a greater understanding of the physiological conditions of E. sinensis varieties with different shell colors by comparing the gut microbiota and gut tissue metabolome. |
| first_indexed | 2024-03-12T23:48:55Z |
| format | Article |
| id | doaj.art-f8e1128948e44847bab93e15640986b8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T23:48:55Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f8e1128948e44847bab93e15640986b82023-07-14T01:54:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12181521218152Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colorsXiaochen Zhu0Yingying Zhao1Yingying Zhao2Na Sun3Changlei Li4Qing Jiang5Yazhao Zhang6Hua Wei7Hua Wei8Yingdong Li9Yingdong Li10Qingbiao Hu11Qingbiao Hu12Xiaodong Li13Xiaodong Li14Xiaodong Li15College of Science and Engineering, Flinders University, Adelaide, SA, AustraliaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaLiaoning Panjin Wetland Ecosystem National Observation and Research Station, Shenyang, ChinaPanjin Guanghe Crab Industry Co. Ltd., Panjin, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaLiaoning Panjin Wetland Ecosystem National Observation and Research Station, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaLiaoning Panjin Wetland Ecosystem National Observation and Research Station, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaLiaoning Panjin Wetland Ecosystem National Observation and Research Station, Shenyang, ChinaCollege of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, ChinaLiaoning Panjin Wetland Ecosystem National Observation and Research Station, Shenyang, ChinaPanjin Guanghe Crab Industry Co. Ltd., Panjin, ChinaIntroductionThe Chinese mitten crab (Eriocheir sinensis) is a highly valued freshwater crustacean in China. While the natural shell color of E. sinensis is greenish brown (GH), we found a variety with a brownish-orange shell color (RH). Although RH is more expensive, it exhibits a lower molting frequency and growth rate compared with GH, which significantly reduces its yield and hinders large-scale farming. The growth and development of animals are closely related to their gut microbiota and gut tissue metabolic profiles.MethodsIn this study, we compared the gut microbiome communities and metabolic profiles of juvenile RH and GH crabs using 16S rRNA gene sequencing and liquid chromatography–mass spectrometry (LC–MS), respectively.ResultsOur findings indicated that the intestinal microbial composition and metabolic characteristics of E. sinensis differed significantly between RH and GH. At the operational taxonomic unit (OTU) level, the α-diversity of the gut microbiota did not differ significantly between RH and GH, while the β-diversity of the RH gut microbiota was higher than that of the GH gut microbiota. At the species level, the richness of unclassified_c_Alphaproteobacteria was significantly higher in the GH group, while the RH group had a significantly higher richness of three low-abundance species, Flavobacteria bacterium BAL38, Paraburkholderia ferrariae, and uncultured_bacterium_g__Legionella. In the current study, 598 gut tissue metabolites were identified, and 159 metabolites were significantly different between GH and RH. The metabolite profile of RH was characteristic of a low level of most amino acids and lipid metabolites and a high level of several pigments compared with that of GH. These metabolites were enriched in 102 KEGG pathways. Four pathways, including (1) Central carbon metabolism in cancer, (2) protein digestion and absorption, (3) alanine, aspartate and glutamate metabolism, and (4) aminoacyl-tRNA biosynthesis, were significantly enriched. The correlation analysis between metabolites and microbiotas indicated that most key differential metabolites were positively correlated with the abundance of Shewanella_sp_MR-7.DiscussionThis research provided a greater understanding of the physiological conditions of E. sinensis varieties with different shell colors by comparing the gut microbiota and gut tissue metabolome.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1218152/fullChinese mitten crabEriocheir sinensisgut microbiotagut tissue metabolomeshell coloration |
| spellingShingle | Xiaochen Zhu Yingying Zhao Yingying Zhao Na Sun Changlei Li Qing Jiang Yazhao Zhang Hua Wei Hua Wei Yingdong Li Yingdong Li Qingbiao Hu Qingbiao Hu Xiaodong Li Xiaodong Li Xiaodong Li Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors Frontiers in Microbiology Chinese mitten crab Eriocheir sinensis gut microbiota gut tissue metabolome shell coloration |
| title | Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors |
| title_full | Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors |
| title_fullStr | Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors |
| title_full_unstemmed | Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors |
| title_short | Comparison of the gut microbiota and untargeted gut tissue metabolome of Chinese mitten crabs (Eriocheir sinensis) with different shell colors |
| title_sort | comparison of the gut microbiota and untargeted gut tissue metabolome of chinese mitten crabs eriocheir sinensis with different shell colors |
| topic | Chinese mitten crab Eriocheir sinensis gut microbiota gut tissue metabolome shell coloration |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1218152/full |
| work_keys_str_mv | AT xiaochenzhu comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT yingyingzhao comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT yingyingzhao comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT nasun comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT changleili comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT qingjiang comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT yazhaozhang comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT huawei comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT huawei comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT yingdongli comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT yingdongli comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT qingbiaohu comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT qingbiaohu comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT xiaodongli comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT xiaodongli comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors AT xiaodongli comparisonofthegutmicrobiotaanduntargetedguttissuemetabolomeofchinesemittencrabseriocheirsinensiswithdifferentshellcolors |