Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk
Halari donkey breed is one of the indigenous breeds of India and its population is rapidly decreasing. The Jenny milk is more similar to human milk, exhibits a wide range of probiotic diversity and hypo-allergenicity, especially among infants suffering from cow and buffalo milk protein allergy. Some...
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024010223 |
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| author | Prashant Singh Anuradha Bhardwaj Varij Nayan Ram Avatar Legha Yash Pal Sonali Soni Shiv Kumar Giri T.K. Bhattacharya |
| author_facet | Prashant Singh Anuradha Bhardwaj Varij Nayan Ram Avatar Legha Yash Pal Sonali Soni Shiv Kumar Giri T.K. Bhattacharya |
| author_sort | Prashant Singh |
| collection | DOAJ |
| description | Halari donkey breed is one of the indigenous breeds of India and its population is rapidly decreasing. The Jenny milk is more similar to human milk, exhibits a wide range of probiotic diversity and hypo-allergenicity, especially among infants suffering from cow and buffalo milk protein allergy. Some studies indicated low levels of κ-casein fraction of casein protein in donkey milk. The k-casein gene was not amplified from the DNA derived from the milk somatic cells of Halari donkeys. The Halari donkey milk has low somatic cells count. We report the first isolation of DNA from milk somatic cells of Halari donkeys with subsequent characterization of k-casein gene. Through our work, we showed that the milk somatic cells can be used as a non-invasive source for DNA isolation towards molecular studies. It also proved the presence of k-casein gene in Halari donkey milk by its amplification from isolated DNA. |
| first_indexed | 2024-03-08T06:54:31Z |
| format | Article |
| id | doaj.art-f8e112ba84254b5c948f8e5acb901f20 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:54:31Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f8e112ba84254b5c948f8e5acb901f202024-02-03T06:38:48ZengElsevierHeliyon2405-84402024-01-01102e24991Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milkPrashant Singh0Anuradha Bhardwaj1Varij Nayan2Ram Avatar Legha3Yash Pal4Sonali Soni5Shiv Kumar Giri6T.K. Bhattacharya7ICAR-National Research Centre on Equines, Hisar 125001 (Haryana), IndiaICAR-National Research Centre on Equines, Hisar 125001 (Haryana), India; Corresponding author. ICAR-National Research Centre on Equines, Sirsa Road, Hisar-125001, Haryana, India.ICAR- Central Institute for Research on Buffaloes, Hisar 125001 (Haryana), IndiaICAR-National Research Centre on Equines, Hisar 125001 (Haryana), IndiaICAR-National Research Centre on Equines, Hisar 125001 (Haryana), IndiaICAR-National Research Centre on Equines, Hisar 125001 (Haryana), India; Department of Biotechnology (SBAS), Maharaja Agrasen University, Baddi 174103 (Solan) HP, IndiaDepartment of Biotechnology (SBAS), Maharaja Agrasen University, Baddi 174103 (Solan) HP, IndiaICAR-National Research Centre on Equines, Hisar 125001 (Haryana), IndiaHalari donkey breed is one of the indigenous breeds of India and its population is rapidly decreasing. The Jenny milk is more similar to human milk, exhibits a wide range of probiotic diversity and hypo-allergenicity, especially among infants suffering from cow and buffalo milk protein allergy. Some studies indicated low levels of κ-casein fraction of casein protein in donkey milk. The k-casein gene was not amplified from the DNA derived from the milk somatic cells of Halari donkeys. The Halari donkey milk has low somatic cells count. We report the first isolation of DNA from milk somatic cells of Halari donkeys with subsequent characterization of k-casein gene. Through our work, we showed that the milk somatic cells can be used as a non-invasive source for DNA isolation towards molecular studies. It also proved the presence of k-casein gene in Halari donkey milk by its amplification from isolated DNA.http://www.sciencedirect.com/science/article/pii/S2405844024010223Halari donkeyMilkSomatic cellsCSN3 |
| spellingShingle | Prashant Singh Anuradha Bhardwaj Varij Nayan Ram Avatar Legha Yash Pal Sonali Soni Shiv Kumar Giri T.K. Bhattacharya Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk Heliyon Halari donkey Milk Somatic cells CSN3 |
| title | Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk |
| title_full | Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk |
| title_fullStr | Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk |
| title_full_unstemmed | Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk |
| title_short | Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk |
| title_sort | milk somatic cell dna isolation and characterization of κ casein gene in halari donkey milk |
| topic | Halari donkey Milk Somatic cells CSN3 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024010223 |
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