A degron-based strategy reveals new insights into Aurora B function in C. elegans.
The widely conserved kinase Aurora B regulates important events during cell division. Surprisingly, recent work has uncovered a few functions of Aurora-family kinases that do not require kinase activity. Thus, understanding this important class of cell cycle regulators will require strategies to dis...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-05-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009567 |
| _version_ | 1819016890481115136 |
|---|---|
| author | Nikita S Divekar Amanda C Davis-Roca Liangyu Zhang Abby F Dernburg Sarah M Wignall |
| author_facet | Nikita S Divekar Amanda C Davis-Roca Liangyu Zhang Abby F Dernburg Sarah M Wignall |
| author_sort | Nikita S Divekar |
| collection | DOAJ |
| description | The widely conserved kinase Aurora B regulates important events during cell division. Surprisingly, recent work has uncovered a few functions of Aurora-family kinases that do not require kinase activity. Thus, understanding this important class of cell cycle regulators will require strategies to distinguish kinase-dependent from independent functions. Here, we address this need in C. elegans by combining germline-specific, auxin-induced Aurora B (AIR-2) degradation with the transgenic expression of kinase-inactive AIR-2. Through this approach, we find that kinase activity is essential for AIR-2's major meiotic functions and also for mitotic chromosome segregation. Moreover, our analysis revealed insight into the assembly of the ring complex (RC), a structure that is essential for chromosome congression in C. elegans oocytes. AIR-2 localizes to chromosomes and recruits other components to form the RC. However, we found that while kinase-dead AIR-2 could load onto chromosomes, other components were not recruited. This failure in RC assembly appeared to be due to a loss of RC SUMOylation, suggesting that there is crosstalk between SUMOylation and phosphorylation in building the RC and implicating AIR-2 in regulating the SUMO pathway in oocytes. Similar conditional depletion approaches may reveal new insights into other cell cycle regulators. |
| first_indexed | 2024-12-21T02:54:49Z |
| format | Article |
| id | doaj.art-f8e29355d77d4d189240f4e4653422db |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-21T02:54:49Z |
| publishDate | 2021-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-f8e29355d77d4d189240f4e4653422db2022-12-21T19:18:22ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-05-01175e100956710.1371/journal.pgen.1009567A degron-based strategy reveals new insights into Aurora B function in C. elegans.Nikita S DivekarAmanda C Davis-RocaLiangyu ZhangAbby F DernburgSarah M WignallThe widely conserved kinase Aurora B regulates important events during cell division. Surprisingly, recent work has uncovered a few functions of Aurora-family kinases that do not require kinase activity. Thus, understanding this important class of cell cycle regulators will require strategies to distinguish kinase-dependent from independent functions. Here, we address this need in C. elegans by combining germline-specific, auxin-induced Aurora B (AIR-2) degradation with the transgenic expression of kinase-inactive AIR-2. Through this approach, we find that kinase activity is essential for AIR-2's major meiotic functions and also for mitotic chromosome segregation. Moreover, our analysis revealed insight into the assembly of the ring complex (RC), a structure that is essential for chromosome congression in C. elegans oocytes. AIR-2 localizes to chromosomes and recruits other components to form the RC. However, we found that while kinase-dead AIR-2 could load onto chromosomes, other components were not recruited. This failure in RC assembly appeared to be due to a loss of RC SUMOylation, suggesting that there is crosstalk between SUMOylation and phosphorylation in building the RC and implicating AIR-2 in regulating the SUMO pathway in oocytes. Similar conditional depletion approaches may reveal new insights into other cell cycle regulators.https://doi.org/10.1371/journal.pgen.1009567 |
| spellingShingle | Nikita S Divekar Amanda C Davis-Roca Liangyu Zhang Abby F Dernburg Sarah M Wignall A degron-based strategy reveals new insights into Aurora B function in C. elegans. PLoS Genetics |
| title | A degron-based strategy reveals new insights into Aurora B function in C. elegans. |
| title_full | A degron-based strategy reveals new insights into Aurora B function in C. elegans. |
| title_fullStr | A degron-based strategy reveals new insights into Aurora B function in C. elegans. |
| title_full_unstemmed | A degron-based strategy reveals new insights into Aurora B function in C. elegans. |
| title_short | A degron-based strategy reveals new insights into Aurora B function in C. elegans. |
| title_sort | degron based strategy reveals new insights into aurora b function in c elegans |
| url | https://doi.org/10.1371/journal.pgen.1009567 |
| work_keys_str_mv | AT nikitasdivekar adegronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT amandacdavisroca adegronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT liangyuzhang adegronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT abbyfdernburg adegronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT sarahmwignall adegronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT nikitasdivekar degronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT amandacdavisroca degronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT liangyuzhang degronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT abbyfdernburg degronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans AT sarahmwignall degronbasedstrategyrevealsnewinsightsintoaurorabfunctionincelegans |