NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES
The morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 μg/ml for SPEV cell line, human fibrobla...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2014-02-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/no1_2014/pavlovich.pdf |
| _version_ | 1797728037643485184 |
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| author | Pavlovich О. V. |
| author_facet | Pavlovich О. V. |
| author_sort | Pavlovich О. V. |
| collection | DOAJ |
| description | The morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 μg/ml for SPEV cell line, human fibroblasts in suspension were investigated. The localization of Eu2O3 nanoparticles in SPEV cells and fibroblasts was determined by confocal scanning microscopy.
We found that europium oxide nanoparticles at a concentration of 6.8 μg/ml did not have a toxic effect on the cell cultures, luminesce in the visible spectral range and remain for a long time in the cells without a significant decrease in intensity of the luminescence. The localization of nanoparticles in cell cytoplasme but not in nuclei was shown by confocal scanning microscopy. They can be used as nanoluminophores for labelling of cell suspensions and cultures. |
| first_indexed | 2024-03-12T11:09:05Z |
| format | Article |
| id | doaj.art-f8ed3a4012664d45b697b530e678f47b |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T11:09:05Z |
| publishDate | 2014-02-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-f8ed3a4012664d45b697b530e678f47b2023-09-02T03:26:41ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2014-02-017111712210.15407/biotech7.01.117NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURESPavlovich О. V.0Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, KharkіvThe morphological and functional characteristics of the cells in cultures in the presence of nanoparticles of Eu2O3 and luminescence characteristics of nanoparticles in cells and also the possible toxic effect of nanoparticles of Eu2O3 in the range of 6.8–340 μg/ml for SPEV cell line, human fibroblasts in suspension were investigated. The localization of Eu2O3 nanoparticles in SPEV cells and fibroblasts was determined by confocal scanning microscopy. We found that europium oxide nanoparticles at a concentration of 6.8 μg/ml did not have a toxic effect on the cell cultures, luminesce in the visible spectral range and remain for a long time in the cells without a significant decrease in intensity of the luminescence. The localization of nanoparticles in cell cytoplasme but not in nuclei was shown by confocal scanning microscopy. They can be used as nanoluminophores for labelling of cell suspensions and cultures.http://biotechnology.kiev.ua/images/storage/no1_2014/pavlovich.pdfSPEV cell linefibroblastsnanoparticlesluminophores |
| spellingShingle | Pavlovich О. V. NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES Biotechnologia Acta SPEV cell line fibroblasts nanoparticles luminophores |
| title | NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES |
| title_full | NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES |
| title_fullStr | NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES |
| title_full_unstemmed | NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES |
| title_short | NANOPARTICLES OF EUROPIUM OXIDE AS FLUORESCENT LABLES OF THE CELL CULTURES |
| title_sort | nanoparticles of europium oxide as fluorescent lables of the cell cultures |
| topic | SPEV cell line fibroblasts nanoparticles luminophores |
| url | http://biotechnology.kiev.ua/images/storage/no1_2014/pavlovich.pdf |
| work_keys_str_mv | AT pavlovichov nanoparticlesofeuropiumoxideasfluorescentlablesofthecellcultures |