Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats
Seminal studies stated that bean proteins are efficient neuronal tracers with affinity for brain tissue. A low molecular weight peptide fraction (<3kDa) from Phaseolus vulgaris (PV3) was previously reported to be antioxidant, non-cytotoxic, and capable of reducing reactive oxygen species and incr...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2022-12-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100680&tlng=en |
| _version_ | 1811185895776190464 |
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| author | D. Graziani J.V.V. Ribeiro L.C. Turones E.A. Costa L.L. Reis-Silva E.G. Araújo L.G.F. de Paula M.D. Ferreira-Junior R.M. Gomes H.M. Campos P.C. Ghedini K.A. Batista K.F. Fernandes C.H. Xavier |
| author_facet | D. Graziani J.V.V. Ribeiro L.C. Turones E.A. Costa L.L. Reis-Silva E.G. Araújo L.G.F. de Paula M.D. Ferreira-Junior R.M. Gomes H.M. Campos P.C. Ghedini K.A. Batista K.F. Fernandes C.H. Xavier |
| author_sort | D. Graziani |
| collection | DOAJ |
| description | Seminal studies stated that bean proteins are efficient neuronal tracers with affinity for brain tissue. A low molecular weight peptide fraction (<3kDa) from Phaseolus vulgaris (PV3) was previously reported to be antioxidant, non-cytotoxic, and capable of reducing reactive oxygen species and increasing nitric oxide in cells. We evaluated the effects of PV3 (5, 50, 100, 500, and 5000 µg/kg) on behavior and the molecular routes potentially involved. Acute and chronic PV3 treatments were performed before testing Wistar rats: i) in the elevated plus-maze (EPM) to assess the anxiolytic-like effect; ii) in the open field (OF) to evaluate locomotion and exploration; and iii) for depression-like behavior in forced swimming (FS). Catecholaminergic involvement was tested using the tyrosine hydroxylases (TH) enzyme inhibitor, α-methyl-DL-tyrosine (AMPT). Brain areas of chronically treated groups were dissected to assess: i) lipid peroxidation (LPO); ii) carbonylated proteins (CP); iii) superoxide dismutase (SOD) and catalase (CAT) enzymatic activities. Neuronal nitric oxide synthases (nNOS) and argininosuccinate synthase (ASS) protein expression was evaluated by western blotting. Acute treatment with PV3 increased the frequency and time spent in the EPM open arms, suggesting anxiolysis. PV3 increased crossing episodes in the OF. These PV3 effects on anxiety and locomotion were absent in the chronically treated group. Acute and chronic PV3 treatments reduced the immobility time in the FS test, suggesting an antidepressant effect. TH inhibition by AMPT reverted acute PV3 effects. PV3 decreased LPO and CP levels and SOD and CAT activities, whereas nNOS and ASS were reduced in few brain areas. In conclusion, PV3 displayed central antioxidant actions that are concomitant to catecholaminergic-dependent anxiolytic and antidepressant effects. |
| first_indexed | 2024-04-11T13:38:12Z |
| format | Article |
| id | doaj.art-f8ee93960bbf4ded9223ebdc9c6ab450 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T13:38:12Z |
| publishDate | 2022-12-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-f8ee93960bbf4ded9223ebdc9c6ab4502022-12-22T04:21:24ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2022-12-015510.1590/1414-431x2022e12314Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in ratsD. Grazianihttps://orcid.org/0000-0002-4997-2658J.V.V. Ribeirohttps://orcid.org/0000-0003-3937-1071L.C. Turoneshttps://orcid.org/0000-0003-4646-5682E.A. Costahttps://orcid.org/0000-0003-1996-0901L.L. Reis-Silvahttps://orcid.org/0000-0003-1799-1106E.G. Araújohttps://orcid.org/0000-0002-0242-2827L.G.F. de Paulahttps://orcid.org/0000-0002-6058-0495M.D. Ferreira-Juniorhttps://orcid.org/0000-0003-0490-1212R.M. Gomeshttps://orcid.org/0000-0002-9012-3287H.M. Camposhttps://orcid.org/0000-0001-8032-3771P.C. Ghedinihttps://orcid.org/0000-0003-2104-4064K.A. Batistahttps://orcid.org/0000-0003-4396-032XK.F. Fernandeshttps://orcid.org/0000-0002-6558-5447C.H. Xavierhttps://orcid.org/0000-0003-4006-8213Seminal studies stated that bean proteins are efficient neuronal tracers with affinity for brain tissue. A low molecular weight peptide fraction (<3kDa) from Phaseolus vulgaris (PV3) was previously reported to be antioxidant, non-cytotoxic, and capable of reducing reactive oxygen species and increasing nitric oxide in cells. We evaluated the effects of PV3 (5, 50, 100, 500, and 5000 µg/kg) on behavior and the molecular routes potentially involved. Acute and chronic PV3 treatments were performed before testing Wistar rats: i) in the elevated plus-maze (EPM) to assess the anxiolytic-like effect; ii) in the open field (OF) to evaluate locomotion and exploration; and iii) for depression-like behavior in forced swimming (FS). Catecholaminergic involvement was tested using the tyrosine hydroxylases (TH) enzyme inhibitor, α-methyl-DL-tyrosine (AMPT). Brain areas of chronically treated groups were dissected to assess: i) lipid peroxidation (LPO); ii) carbonylated proteins (CP); iii) superoxide dismutase (SOD) and catalase (CAT) enzymatic activities. Neuronal nitric oxide synthases (nNOS) and argininosuccinate synthase (ASS) protein expression was evaluated by western blotting. Acute treatment with PV3 increased the frequency and time spent in the EPM open arms, suggesting anxiolysis. PV3 increased crossing episodes in the OF. These PV3 effects on anxiety and locomotion were absent in the chronically treated group. Acute and chronic PV3 treatments reduced the immobility time in the FS test, suggesting an antidepressant effect. TH inhibition by AMPT reverted acute PV3 effects. PV3 decreased LPO and CP levels and SOD and CAT activities, whereas nNOS and ASS were reduced in few brain areas. In conclusion, PV3 displayed central antioxidant actions that are concomitant to catecholaminergic-dependent anxiolytic and antidepressant effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100680&tlng=enBeanPhaseolus vulgarisPeptidesAnxietyDepressionNutraceutical |
| spellingShingle | D. Graziani J.V.V. Ribeiro L.C. Turones E.A. Costa L.L. Reis-Silva E.G. Araújo L.G.F. de Paula M.D. Ferreira-Junior R.M. Gomes H.M. Campos P.C. Ghedini K.A. Batista K.F. Fernandes C.H. Xavier Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats Brazilian Journal of Medical and Biological Research Bean Phaseolus vulgaris Peptides Anxiety Depression Nutraceutical |
| title | Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats |
| title_full | Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats |
| title_fullStr | Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats |
| title_full_unstemmed | Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats |
| title_short | Behavioral effects of a low molecular weight peptide fraction from Phaseolus vulgaris in rats |
| title_sort | behavioral effects of a low molecular weight peptide fraction from phaseolus vulgaris in rats |
| topic | Bean Phaseolus vulgaris Peptides Anxiety Depression Nutraceutical |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100680&tlng=en |
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