Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis
PCR and FC-ALTA were used to monitor parasite clearance in 54 chronic chagasic patients who had completed therapy with allopurinol (ALLO, n = 31) or itraconazole (ITRA, n = 23) ten years earlier. All patients maintained positive conventional serology. 25 of them showed positive XD (ALLO, n = 11 and...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2005-12-01
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| Series: | Parasite |
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| Online Access: | http://dx.doi.org/10.1051/parasite/2005124353 |
| _version_ | 1797430506388717568 |
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| author | Sánchez G. Coronado X. Zulantay I. Apt W. Gajardo M. Solari S. Venegas J. |
| author_facet | Sánchez G. Coronado X. Zulantay I. Apt W. Gajardo M. Solari S. Venegas J. |
| author_sort | Sánchez G. |
| collection | DOAJ |
| description | PCR and FC-ALTA were used to monitor parasite clearance in 54 chronic chagasic patients who had completed therapy with allopurinol (ALLO, n = 31) or itraconazole (ITRA, n = 23) ten years earlier. All patients maintained positive conventional serology. 25 of them showed positive XD (ALLO, n = 11 and ITRA, n = 14) and 29 negative XD (ALLO, n = 20 and ITRA, n = 9). 43 patients were positive by both techniques (ALLO, n = 23 and ITRA, n = 20). Seven of 54 patients were negative by PCR and positive by FC-ALTA and three of 54 were positive by PCR and negative by FC-ALTA. Only one case with both tests negative should be considered cured. Of 29 patients with negative XD, 14 treated ALLO (70 %) and nine with ITRA (77.8 %) showed positive PCR and FC-ALTA. These results do not show differences of efficacy among the drugs, and reinforce the relevance of using sensitive tools such as PCR and FC-ALTA for the follow-up of patients with chronic Chagas disease. |
| first_indexed | 2024-03-09T09:28:30Z |
| format | Article |
| id | doaj.art-f8eefa78ac3a4a37a4a09ebc13d51037 |
| institution | Directory Open Access Journal |
| issn | 1252-607X 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T09:28:30Z |
| publishDate | 2005-12-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-f8eefa78ac3a4a37a4a09ebc13d510372023-12-02T05:28:29ZengEDP SciencesParasite1252-607X1776-10422005-12-0112435335710.1051/parasite/2005124353parasite2005124p353Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysisSánchez G.Coronado X.Zulantay I.Apt W.Gajardo M.Solari S.Venegas J.PCR and FC-ALTA were used to monitor parasite clearance in 54 chronic chagasic patients who had completed therapy with allopurinol (ALLO, n = 31) or itraconazole (ITRA, n = 23) ten years earlier. All patients maintained positive conventional serology. 25 of them showed positive XD (ALLO, n = 11 and ITRA, n = 14) and 29 negative XD (ALLO, n = 20 and ITRA, n = 9). 43 patients were positive by both techniques (ALLO, n = 23 and ITRA, n = 20). Seven of 54 patients were negative by PCR and positive by FC-ALTA and three of 54 were positive by PCR and negative by FC-ALTA. Only one case with both tests negative should be considered cured. Of 29 patients with negative XD, 14 treated ALLO (70 %) and nine with ITRA (77.8 %) showed positive PCR and FC-ALTA. These results do not show differences of efficacy among the drugs, and reinforce the relevance of using sensitive tools such as PCR and FC-ALTA for the follow-up of patients with chronic Chagas disease.http://dx.doi.org/10.1051/parasite/2005124353chronic Chagas diseasepolymerase chain reaction (PCR)flow cytometry anti-live trypomastigote antibodies (FC-ALTA) |
| spellingShingle | Sánchez G. Coronado X. Zulantay I. Apt W. Gajardo M. Solari S. Venegas J. Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis Parasite chronic Chagas disease polymerase chain reaction (PCR) flow cytometry anti-live trypomastigote antibodies (FC-ALTA) |
| title | Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis |
| title_full | Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis |
| title_fullStr | Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis |
| title_full_unstemmed | Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis |
| title_short | Monitoring the efficacy of specific treatment in chronic Chagas disease by polymerase chain reaction and flow cytometry analysis |
| title_sort | monitoring the efficacy of specific treatment in chronic chagas disease by polymerase chain reaction and flow cytometry analysis |
| topic | chronic Chagas disease polymerase chain reaction (PCR) flow cytometry anti-live trypomastigote antibodies (FC-ALTA) |
| url | http://dx.doi.org/10.1051/parasite/2005124353 |
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