Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides
CVN (cyanovirin-N) is an anti-HIV protein. CVNH (cyanovirin-N homology) represents its homology. In a previous study, we first reported the full-length sequences of the CVNH gene cloned from Ceratopteris thalictroides. Based on the finding, the coding sequence of CtCVNH was optimized in the study, a...
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| Format: | Article |
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MDPI AG
2013-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/14/4/7506 |
| _version_ | 1811289868259557376 |
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| author | Ting Wang Yingjuan Su Junbo Sun |
| author_facet | Ting Wang Yingjuan Su Junbo Sun |
| author_sort | Ting Wang |
| collection | DOAJ |
| description | CVN (cyanovirin-N) is an anti-HIV protein. CVNH (cyanovirin-N homology) represents its homology. In a previous study, we first reported the full-length sequences of the CVNH gene cloned from Ceratopteris thalictroides. Based on the finding, the coding sequence of CtCVNH was optimized in the study, and then a pET prokaryotic expression vector was constructed. The purification and identification of CtCVNH protein were investigated, as well. SDS-PAGE analysis indicated that a 31 kDa protein was overexpressed and mainly accumulated in the soluble fraction. Only a single protein was obtained after the Ni- nitrilotriacetic acid (NTA) affinity chromatography. The purified protein was identified to be the recombinant CtCVNH by both Western blot and peptide mass fingerprinting analysis. |
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| id | doaj.art-f8f00241fdb449799af2bfde84304ca1 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T04:01:39Z |
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| spelling | doaj.art-f8f00241fdb449799af2bfde84304ca12022-12-22T03:03:28ZengMDPI AGInternational Journal of Molecular Sciences1422-00672013-04-011447506751410.3390/ijms14047506Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroidesTing WangYingjuan SuJunbo SunCVN (cyanovirin-N) is an anti-HIV protein. CVNH (cyanovirin-N homology) represents its homology. In a previous study, we first reported the full-length sequences of the CVNH gene cloned from Ceratopteris thalictroides. Based on the finding, the coding sequence of CtCVNH was optimized in the study, and then a pET prokaryotic expression vector was constructed. The purification and identification of CtCVNH protein were investigated, as well. SDS-PAGE analysis indicated that a 31 kDa protein was overexpressed and mainly accumulated in the soluble fraction. Only a single protein was obtained after the Ni- nitrilotriacetic acid (NTA) affinity chromatography. The purified protein was identified to be the recombinant CtCVNH by both Western blot and peptide mass fingerprinting analysis.http://www.mdpi.com/1422-0067/14/4/7506Ceratopteris thalictroidesCtCVNHanti-HIV proteinprokaryotic expression |
| spellingShingle | Ting Wang Yingjuan Su Junbo Sun Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides International Journal of Molecular Sciences Ceratopteris thalictroides CtCVNH anti-HIV protein prokaryotic expression |
| title | Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides |
| title_full | Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides |
| title_fullStr | Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides |
| title_full_unstemmed | Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides |
| title_short | Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides |
| title_sort | expression purification and identification of ctcvnh a novel anti hiv human immunodeficiency virus protein from ceratopteris thalictroides |
| topic | Ceratopteris thalictroides CtCVNH anti-HIV protein prokaryotic expression |
| url | http://www.mdpi.com/1422-0067/14/4/7506 |
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