Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice
<p>Abstract</p> <p>Background</p> <p>Surfactant protein-A (SP-A) has been shown to play a variety of roles related to lung host defense function. Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) pro...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/8/1/34 |
| _version_ | 1811282851971203072 |
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| author | Mikerov Anatoly N Haque Rizwanul Umstead Todd M Ali Mehboob Freeman Willard M Floros Joanna Phelps David S |
| author_facet | Mikerov Anatoly N Haque Rizwanul Umstead Todd M Ali Mehboob Freeman Willard M Floros Joanna Phelps David S |
| author_sort | Mikerov Anatoly N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Surfactant protein-A (SP-A) has been shown to play a variety of roles related to lung host defense function. Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with <it>Klebsiella pneumoniae </it>by 2D-DIGE.</p> <p>Methods</p> <p>Mice were infected intratracheally with <it>K. pneumoniae </it>and after 4 and 24 hours they were subject to BAL. Cell-free BAL was analyzed by 2D-DIGE on two-dimensional gels with pH ranges of 4-7 and 7-11. Under baseline conditions and at 4 and 24 hr post-infection BAL was compared between untreated and infected wild type and SP-A-/- mice. Sixty proteins identified by mass spectrometry were categorized as host defense, redox regulation, and protein metabolism/modification.</p> <p>Results</p> <p>We found: 1) ~75% of 32 host defense proteins were lower in uninfected SP-A-/- vs wild type, suggesting increased susceptibility to infection or oxidative injury; 2) At 4 hr post-infection > 2/3 of identified proteins were higher in SP-A-/- than wild type mice, almost the exact opposite of untreated mice; 3) At 24 hr post-infection some proteins continued increasing, but many returned to baseline; 4) In infected wild type mice significant changes occurred in 13 of 60 proteins, with 12 of 13 increasing, vs on 4 significant changes in SP-A-/- mice. Infection response patterns between strains demonstrated both commonalities and differences. In several cases changes between 4 and 24 hr followed different patterns between strains.</p> <p>Conclusions</p> <p>These indicate that SP-A plays a key role in regulating the BAL proteome, functioning indirectly to regulate lung host defense function, possibly via the macrophage. In the absence of SP-A baseline levels of many host defense molecules are lower. However, many of these indirect deficits in SP-A-/- mice are rapidly compensated for during infection, indicating that SP-A also has a direct role on host defense against <it>K. pneumoniae </it>that may be instrumental in determining clinical course.</p> |
| first_indexed | 2024-04-13T01:59:59Z |
| format | Article |
| id | doaj.art-f8f25f52bb5748b3a1faf0aa243dec88 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-04-13T01:59:59Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-f8f25f52bb5748b3a1faf0aa243dec882022-12-22T03:07:40ZengBMCProteome Science1477-59562010-06-01813410.1186/1477-5956-8-34Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- miceMikerov Anatoly NHaque RizwanulUmstead Todd MAli MehboobFreeman Willard MFloros JoannaPhelps David S<p>Abstract</p> <p>Background</p> <p>Surfactant protein-A (SP-A) has been shown to play a variety of roles related to lung host defense function. Mice lacking SP-A are more susceptible to infection than wild type C57BL/6 mice. We studied bronchoalveolar lavage (BAL) protein expression in wild type and SP-A-/- mice infected with <it>Klebsiella pneumoniae </it>by 2D-DIGE.</p> <p>Methods</p> <p>Mice were infected intratracheally with <it>K. pneumoniae </it>and after 4 and 24 hours they were subject to BAL. Cell-free BAL was analyzed by 2D-DIGE on two-dimensional gels with pH ranges of 4-7 and 7-11. Under baseline conditions and at 4 and 24 hr post-infection BAL was compared between untreated and infected wild type and SP-A-/- mice. Sixty proteins identified by mass spectrometry were categorized as host defense, redox regulation, and protein metabolism/modification.</p> <p>Results</p> <p>We found: 1) ~75% of 32 host defense proteins were lower in uninfected SP-A-/- vs wild type, suggesting increased susceptibility to infection or oxidative injury; 2) At 4 hr post-infection > 2/3 of identified proteins were higher in SP-A-/- than wild type mice, almost the exact opposite of untreated mice; 3) At 24 hr post-infection some proteins continued increasing, but many returned to baseline; 4) In infected wild type mice significant changes occurred in 13 of 60 proteins, with 12 of 13 increasing, vs on 4 significant changes in SP-A-/- mice. Infection response patterns between strains demonstrated both commonalities and differences. In several cases changes between 4 and 24 hr followed different patterns between strains.</p> <p>Conclusions</p> <p>These indicate that SP-A plays a key role in regulating the BAL proteome, functioning indirectly to regulate lung host defense function, possibly via the macrophage. In the absence of SP-A baseline levels of many host defense molecules are lower. However, many of these indirect deficits in SP-A-/- mice are rapidly compensated for during infection, indicating that SP-A also has a direct role on host defense against <it>K. pneumoniae </it>that may be instrumental in determining clinical course.</p>http://www.proteomesci.com/content/8/1/34 |
| spellingShingle | Mikerov Anatoly N Haque Rizwanul Umstead Todd M Ali Mehboob Freeman Willard M Floros Joanna Phelps David S Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice Proteome Science |
| title | Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice |
| title_full | Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice |
| title_fullStr | Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice |
| title_full_unstemmed | Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice |
| title_short | Differences in the BAL proteome after <it>Klebsiella pneumoniae </it>infection in wild type and SP-A-/- mice |
| title_sort | differences in the bal proteome after it klebsiella pneumoniae it infection in wild type and sp a mice |
| url | http://www.proteomesci.com/content/8/1/34 |
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