Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation
Poor solubility and short biological half-life present a challenge that needs to be overcome in order to improve the recognized bioactivities of curcumin (CUR), the main phenolic compounds derived from the roots of <i>Curcuma longa</i>. However, drug delivery systems have proven to be an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/10/2431 |
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| author | María Isabel Quirós-Fallas Krissia Wilhelm-Romero Silvia Quesada-Mora Gabriela Azofeifa-Cordero Luis Felipe Vargas-Huertas Diego Alvarado-Corella Juan José Mora-Román José Roberto Vega-Baudrit Mirtha Navarro-Hoyos Andrea Mariela Araya-Sibaja |
| author_facet | María Isabel Quirós-Fallas Krissia Wilhelm-Romero Silvia Quesada-Mora Gabriela Azofeifa-Cordero Luis Felipe Vargas-Huertas Diego Alvarado-Corella Juan José Mora-Román José Roberto Vega-Baudrit Mirtha Navarro-Hoyos Andrea Mariela Araya-Sibaja |
| author_sort | María Isabel Quirós-Fallas |
| collection | DOAJ |
| description | Poor solubility and short biological half-life present a challenge that needs to be overcome in order to improve the recognized bioactivities of curcumin (CUR), the main phenolic compounds derived from the roots of <i>Curcuma longa</i>. However, drug delivery systems have proven to be an excellent strategy to improve and obtain greater bioavailability. Our previous studies on curcuminoid hybrid nanoparticles have shown promising results by significantly increasing the solubility of desmethoxycurcumin (DMC) and bisdemethoxycurcumin (BDM). In this contribution, we performed a detailed characterization of a CUR as well as in vitro and in vivo studies. The developed method produced CUR loaded nanoparticles with an average size of 49.46 ± 0.80. Moreover, the FT-IR analysis confirmed the encapsulation, and TEM images showed their spherical shape. The NP achieved an encapsulation efficiency greater than 99%. Further, the release studies found that the NPs obtained a significantly higher release than the pure compounds in water. In vivo delayed-type hypersensitivity (DTH) studies showed promising results by enhancing the immune activity response of CUR in NP compared to bulk CUR. Furthermore, we report a significant increase in antioxidant activity for CUR-NP in aqueous solution compared to free CUR. Finally, an important in vitro cytotoxic effect on gastric AGS and colon SW620 adenocarcinoma cell lines was found for CUR-NP while empty carrier nanoparticles are observed to exhibit low cytotoxicity, indicating the potential of these CUR-PLU NPs for further studies to assess their phytotherapeutic applications. |
| first_indexed | 2024-03-09T20:39:27Z |
| format | Article |
| id | doaj.art-f8f50ab2465b45e8a894d9ee8e28b948 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:39:27Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-f8f50ab2465b45e8a894d9ee8e28b9482023-11-23T23:02:57ZengMDPI AGBiomedicines2227-90592022-09-011010243110.3390/biomedicines10102431Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity EvaluationMaría Isabel Quirós-Fallas0Krissia Wilhelm-Romero1Silvia Quesada-Mora2Gabriela Azofeifa-Cordero3Luis Felipe Vargas-Huertas4Diego Alvarado-Corella5Juan José Mora-Román6José Roberto Vega-Baudrit7Mirtha Navarro-Hoyos8Andrea Mariela Araya-Sibaja9Laboratorio BIODESS, Escuela de Química, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaLaboratorio BIODESS, Escuela de Química, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaDepartmento de Bioquímica, Escuela de Medicina, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaDepartmento de Bioquímica, Escuela de Medicina, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaLaboratorio BIODESS, Escuela de Química, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaLaboratorio BIODESS, Escuela de Química, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaFacultad de Farmacia, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaLaboratorio Nacional de Nanotecnología LANOTEC-CeNAT-CONARE, Pavas, San José 1174-1200, Costa RicaLaboratorio BIODESS, Escuela de Química, Universidad de Costa Rica, San Pedro de Montes de Oca, San José 2060, Costa RicaLaboratorio Nacional de Nanotecnología LANOTEC-CeNAT-CONARE, Pavas, San José 1174-1200, Costa RicaPoor solubility and short biological half-life present a challenge that needs to be overcome in order to improve the recognized bioactivities of curcumin (CUR), the main phenolic compounds derived from the roots of <i>Curcuma longa</i>. However, drug delivery systems have proven to be an excellent strategy to improve and obtain greater bioavailability. Our previous studies on curcuminoid hybrid nanoparticles have shown promising results by significantly increasing the solubility of desmethoxycurcumin (DMC) and bisdemethoxycurcumin (BDM). In this contribution, we performed a detailed characterization of a CUR as well as in vitro and in vivo studies. The developed method produced CUR loaded nanoparticles with an average size of 49.46 ± 0.80. Moreover, the FT-IR analysis confirmed the encapsulation, and TEM images showed their spherical shape. The NP achieved an encapsulation efficiency greater than 99%. Further, the release studies found that the NPs obtained a significantly higher release than the pure compounds in water. In vivo delayed-type hypersensitivity (DTH) studies showed promising results by enhancing the immune activity response of CUR in NP compared to bulk CUR. Furthermore, we report a significant increase in antioxidant activity for CUR-NP in aqueous solution compared to free CUR. Finally, an important in vitro cytotoxic effect on gastric AGS and colon SW620 adenocarcinoma cell lines was found for CUR-NP while empty carrier nanoparticles are observed to exhibit low cytotoxicity, indicating the potential of these CUR-PLU NPs for further studies to assess their phytotherapeutic applications.https://www.mdpi.com/2227-9059/10/10/2431curcuminhybrid lipid polymeric nanoparticlesImmune cellular responsecytotoxicityantioxidant activitynanoparticle characterization |
| spellingShingle | María Isabel Quirós-Fallas Krissia Wilhelm-Romero Silvia Quesada-Mora Gabriela Azofeifa-Cordero Luis Felipe Vargas-Huertas Diego Alvarado-Corella Juan José Mora-Román José Roberto Vega-Baudrit Mirtha Navarro-Hoyos Andrea Mariela Araya-Sibaja Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation Biomedicines curcumin hybrid lipid polymeric nanoparticles Immune cellular response cytotoxicity antioxidant activity nanoparticle characterization |
| title | Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation |
| title_full | Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation |
| title_fullStr | Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation |
| title_full_unstemmed | Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation |
| title_short | Curcumin Hybrid Lipid Polymeric Nanoparticles: Antioxidant Activity, Immune Cellular Response, and Cytotoxicity Evaluation |
| title_sort | curcumin hybrid lipid polymeric nanoparticles antioxidant activity immune cellular response and cytotoxicity evaluation |
| topic | curcumin hybrid lipid polymeric nanoparticles Immune cellular response cytotoxicity antioxidant activity nanoparticle characterization |
| url | https://www.mdpi.com/2227-9059/10/10/2431 |
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