<i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts
Marine algae have gained much importance in the development of nutraceutical products due to their high content of bioactive compounds. In this work, we investigated the activity of <i>Padina pavonica</i> with the aim to demonstrate the pro-osteogenic ability of its extract on human prim...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/8/473 |
| _version_ | 1798005449793994752 |
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| author | Mariagiulia Minetti Giulia Bernardini Manuele Biazzo Gilles Gutierrez Michela Geminiani Teresa Petrucci Annalisa Santucci |
| author_facet | Mariagiulia Minetti Giulia Bernardini Manuele Biazzo Gilles Gutierrez Michela Geminiani Teresa Petrucci Annalisa Santucci |
| author_sort | Mariagiulia Minetti |
| collection | DOAJ |
| description | Marine algae have gained much importance in the development of nutraceutical products due to their high content of bioactive compounds. In this work, we investigated the activity of <i>Padina pavonica</i> with the aim to demonstrate the pro-osteogenic ability of its extract on human primary osteoblast (HOb). Our data indicated that the acetonic extract of <i>P. pavonica</i> (EPP) is a safe product as it did not show any effect on osteoblast viability. At the same time, EPP showed to possess a beneficial effect on HOb functionality, triggering their differentiation and mineralization abilities. In particular EPP enhanced the expression of the earlier differentiation stage markers: a 5.4-fold increase in collagen type I alpha 1 chain (COL1A1), and a 2.3-fold increase in alkaline phosphatase (ALPL), as well as those involved in the late differentiation stage: a 3.7-fold increase in osteocalcin (BGLAP) expression and a 2.8-fold in osteoprotegerin (TNFRSF11B). These findings were corroborated by the enhancement in ALPL enzymatic activity (1.7-fold increase) and by the reduction of receptor activator of nuclear factor-<i>κ</i>B ligand (RANKL) and osteoprotegerin (OPG) ratio (0.6-fold decrease). Moreover, EPP demonstrated the capacity to enhance the bone nodules formation by 3.2-fold in 4 weeks treated HOb. Therefore, EPP showed a significant capability of promoting osteoblast phenotype. Given its positive effect on bone homeostasis, EPP could be used as a useful nutraceutical product that, in addition to a healthy lifestyle and diet, can be able to contrast and prevent bone diseases, especially those connected with ageing, such as osteoporosis (OP). |
| first_indexed | 2024-04-11T12:39:25Z |
| format | Article |
| id | doaj.art-f8fa40efc499401ba5f6cffbb8454058 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:39:25Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f8fa40efc499401ba5f6cffbb84540582022-12-22T04:23:32ZengMDPI AGMarine Drugs1660-33972019-08-0117847310.3390/md17080473md17080473<i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary OsteoblastsMariagiulia Minetti0Giulia Bernardini1Manuele Biazzo2Gilles Gutierrez3Michela Geminiani4Teresa Petrucci5Annalisa Santucci6Dipartimento di Biotecnologie, Chimica e Farmacia (Dipartimento di Eccellenza 2018-2022), Università degli Studi di Siena, via Aldo Moro 2, 53100 Siena, ItalyDipartimento di Biotecnologie, Chimica e Farmacia (Dipartimento di Eccellenza 2018-2022), Università degli Studi di Siena, via Aldo Moro 2, 53100 Siena, ItalyInstitute of Cellular Pharmacology (ICP Ltd.), F24, Triq Valletta, Mosta Technopark, Mosta MST 3000, MaltaInstitute of Cellular Pharmacology (ICP Ltd.), F24, Triq Valletta, Mosta Technopark, Mosta MST 3000, MaltaDipartimento di Biotecnologie, Chimica e Farmacia (Dipartimento di Eccellenza 2018-2022), Università degli Studi di Siena, via Aldo Moro 2, 53100 Siena, ItalyDipartimento di Biotecnologie, Chimica e Farmacia (Dipartimento di Eccellenza 2018-2022), Università degli Studi di Siena, via Aldo Moro 2, 53100 Siena, ItalyDipartimento di Biotecnologie, Chimica e Farmacia (Dipartimento di Eccellenza 2018-2022), Università degli Studi di Siena, via Aldo Moro 2, 53100 Siena, ItalyMarine algae have gained much importance in the development of nutraceutical products due to their high content of bioactive compounds. In this work, we investigated the activity of <i>Padina pavonica</i> with the aim to demonstrate the pro-osteogenic ability of its extract on human primary osteoblast (HOb). Our data indicated that the acetonic extract of <i>P. pavonica</i> (EPP) is a safe product as it did not show any effect on osteoblast viability. At the same time, EPP showed to possess a beneficial effect on HOb functionality, triggering their differentiation and mineralization abilities. In particular EPP enhanced the expression of the earlier differentiation stage markers: a 5.4-fold increase in collagen type I alpha 1 chain (COL1A1), and a 2.3-fold increase in alkaline phosphatase (ALPL), as well as those involved in the late differentiation stage: a 3.7-fold increase in osteocalcin (BGLAP) expression and a 2.8-fold in osteoprotegerin (TNFRSF11B). These findings were corroborated by the enhancement in ALPL enzymatic activity (1.7-fold increase) and by the reduction of receptor activator of nuclear factor-<i>κ</i>B ligand (RANKL) and osteoprotegerin (OPG) ratio (0.6-fold decrease). Moreover, EPP demonstrated the capacity to enhance the bone nodules formation by 3.2-fold in 4 weeks treated HOb. Therefore, EPP showed a significant capability of promoting osteoblast phenotype. Given its positive effect on bone homeostasis, EPP could be used as a useful nutraceutical product that, in addition to a healthy lifestyle and diet, can be able to contrast and prevent bone diseases, especially those connected with ageing, such as osteoporosis (OP).https://www.mdpi.com/1660-3397/17/8/473<i>Padina pavonica</i>marine algaeosteoporosisbone metabolismbone healthnutraceutical |
| spellingShingle | Mariagiulia Minetti Giulia Bernardini Manuele Biazzo Gilles Gutierrez Michela Geminiani Teresa Petrucci Annalisa Santucci <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts Marine Drugs <i>Padina pavonica</i> marine algae osteoporosis bone metabolism bone health nutraceutical |
| title | <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts |
| title_full | <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts |
| title_fullStr | <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts |
| title_full_unstemmed | <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts |
| title_short | <i>Padina pavonica</i> Extract Promotes In Vitro Differentiation and Functionality of Human Primary Osteoblasts |
| title_sort | i padina pavonica i extract promotes in vitro differentiation and functionality of human primary osteoblasts |
| topic | <i>Padina pavonica</i> marine algae osteoporosis bone metabolism bone health nutraceutical |
| url | https://www.mdpi.com/1660-3397/17/8/473 |
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