Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata
ABSTRACT: Objectives: Infections due to Candida species represent a serious threat to healthcare facilities. Only a few classes of antifungal agents are available, and the rapid emergence of (multi)drug resistance eliminates effective treatment options for successful patient outcome. Topically appl...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716522000340 |
| _version_ | 1828727145170468864 |
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| author | Kathrin Spettel Dominik Bumberger Iris Camp Richard Kriz Birgit Willinger |
| author_facet | Kathrin Spettel Dominik Bumberger Iris Camp Richard Kriz Birgit Willinger |
| author_sort | Kathrin Spettel |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Infections due to Candida species represent a serious threat to healthcare facilities. Only a few classes of antifungal agents are available, and the rapid emergence of (multi)drug resistance eliminates effective treatment options for successful patient outcome. Topically applied antiseptics may represent a suitable tool for infection control and local therapy. This study aimed to investigate the in vitro efficacy of the widely used antiseptic octenidine (OCT) against clinical isolates of emerging azole-, echinocandin- and multi-resistant Candida albicans and Candida glabrata. Methods: The antifungal activity of different concentrations of OCT ranging from 0.001% to 0.05% and of OCT-containing ready-to-use products was determined against well-characterised (multidrug) resistant C. albicans and C. glabrata isolates, including susceptible wild-type strains. Quantitative suspension tests were performed under “clean conditions” (0.3 g/L bovine serum albumin) and under “dirty conditions” (3 g/L albumin + 3 mL/L defibrinated sheep blood) as well as various contact times (30 s, 1 min, 2 min) according to EN13624:2013. Results: Even in the presence of a high organic load, pure OCT at 0.05% and a contact time of 30 s was fully effective for all Candida strains, with growth kinetics indicating a time- and concentration-dependent activity. Importantly, commercially available OCT-based products achieved the required reduction of ≥4 log10 for all Candida isolates under the most challenging dirty conditions within two minutes, which makes them suitable for routine clinical use. Conclusion: These results encourage consideration of the well-tolerated antiseptic molecule OCT in the eradication of emerging (multidrug) resistant C. albicans and C. glabrata. |
| first_indexed | 2024-04-12T13:53:36Z |
| format | Article |
| id | doaj.art-f8fa6cd569c24c8cb92c07d494f38f49 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-04-12T13:53:36Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-f8fa6cd569c24c8cb92c07d494f38f492022-12-22T03:30:26ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-01292328Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrataKathrin Spettel0Dominik Bumberger1Iris Camp2Richard Kriz3Birgit Willinger4Medical University of Vienna, Department of Laboratory Medicine, Division of Clinical Microbiology, AustriaMedical University of Vienna, Department of Laboratory Medicine, Division of Clinical Microbiology, AustriaMedical University of Vienna, Department of Laboratory Medicine, Division of Clinical Microbiology, AustriaMedical University of Vienna, Department of Medicine I, Division of Infectious Diseases and Tropical Medicine, AustriaMedical University of Vienna, Department of Laboratory Medicine, Division of Clinical Microbiology, Austria; Corresponding author at: Birgit Willinger, Division of Clinical Microbiology, Department of Laboratory Medicine, Medical University of Vienna, Waehringer Guertel 18-20/5P, 1090 Vienna, Austria.ABSTRACT: Objectives: Infections due to Candida species represent a serious threat to healthcare facilities. Only a few classes of antifungal agents are available, and the rapid emergence of (multi)drug resistance eliminates effective treatment options for successful patient outcome. Topically applied antiseptics may represent a suitable tool for infection control and local therapy. This study aimed to investigate the in vitro efficacy of the widely used antiseptic octenidine (OCT) against clinical isolates of emerging azole-, echinocandin- and multi-resistant Candida albicans and Candida glabrata. Methods: The antifungal activity of different concentrations of OCT ranging from 0.001% to 0.05% and of OCT-containing ready-to-use products was determined against well-characterised (multidrug) resistant C. albicans and C. glabrata isolates, including susceptible wild-type strains. Quantitative suspension tests were performed under “clean conditions” (0.3 g/L bovine serum albumin) and under “dirty conditions” (3 g/L albumin + 3 mL/L defibrinated sheep blood) as well as various contact times (30 s, 1 min, 2 min) according to EN13624:2013. Results: Even in the presence of a high organic load, pure OCT at 0.05% and a contact time of 30 s was fully effective for all Candida strains, with growth kinetics indicating a time- and concentration-dependent activity. Importantly, commercially available OCT-based products achieved the required reduction of ≥4 log10 for all Candida isolates under the most challenging dirty conditions within two minutes, which makes them suitable for routine clinical use. Conclusion: These results encourage consideration of the well-tolerated antiseptic molecule OCT in the eradication of emerging (multidrug) resistant C. albicans and C. glabrata.http://www.sciencedirect.com/science/article/pii/S2213716522000340CandidaResistanceAntisepticsOctenidine |
| spellingShingle | Kathrin Spettel Dominik Bumberger Iris Camp Richard Kriz Birgit Willinger Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata Journal of Global Antimicrobial Resistance Candida Resistance Antiseptics Octenidine |
| title | Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata |
| title_full | Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata |
| title_fullStr | Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata |
| title_full_unstemmed | Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata |
| title_short | Efficacy of octenidine against emerging echinocandin-, azole- and multidrug-resistant Candida albicans and Candida glabrata |
| title_sort | efficacy of octenidine against emerging echinocandin azole and multidrug resistant candida albicans and candida glabrata |
| topic | Candida Resistance Antiseptics Octenidine |
| url | http://www.sciencedirect.com/science/article/pii/S2213716522000340 |
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