A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice
To explore the relationship between diabetes and intervertebral disc degeneration in mice and the associated underlying mechanism. Four-week-old male Kunming mice were used to model diabetes using a high-fat diet combined with streptozotocin injection. After 6 months, morphological and pathological...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023005029 |
| _version_ | 1811161794732883968 |
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| author | Huilin Quan Xiaoshuang Zuo Yu Huan Xuankang Wang Zhou Yao Chunmei Wang Fang Ren Hong Wang Hongyan Qin Xueyu Hu |
| author_facet | Huilin Quan Xiaoshuang Zuo Yu Huan Xuankang Wang Zhou Yao Chunmei Wang Fang Ren Hong Wang Hongyan Qin Xueyu Hu |
| author_sort | Huilin Quan |
| collection | DOAJ |
| description | To explore the relationship between diabetes and intervertebral disc degeneration in mice and the associated underlying mechanism. Four-week-old male Kunming mice were used to model diabetes using a high-fat diet combined with streptozotocin injection. After 6 months, morphological and pathological changes in L4-L6 intervertebral discs were detected by magnetic resonance imaging, micro-CT and histological staining. Immunostaining of CD31, F4/80 and CD16/32 receptors was used to detect vascular invasion and inflammatory infiltration in endplates; the exact changes were then explored by the transmission electron microscopy. The nucleus pulposus of the control and the diabetic group had a clear boundary and regular shape without collapse, while endplate calcification and chondrocyte abnormality in the diabetic group were more obvious. Immunofluorescence confirmed that compared to control, expression levels of CD31 (vascular endothelial marker) and F4/80 (monocyte/macrophage marker) in the diabetic group were significantly increased (P < 0.05), with an elevated number of F4/80 (+)/CD16/32 (+) cells (P < 0.05). The morphology of endplates was observed by transmission electron microscopy, which showed monocytes/macrophage accumulation in the endplate of the diabetic group, accompanied by increased vascular density, collagen fiber distortion and chondrocyte abnormality. In a conclusion, diabetes promotes endplate degeneration with vascular invasion, monocyte/macrophage infiltration and inflammation in mice. |
| first_indexed | 2024-04-10T06:21:12Z |
| format | Article |
| id | doaj.art-f8fffa4f824a4d5d9f2cbf07564f5715 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T06:21:12Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f8fffa4f824a4d5d9f2cbf07564f57152023-03-02T05:00:52ZengElsevierHeliyon2405-84402023-02-0192e13295A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in miceHuilin Quan0Xiaoshuang Zuo1Yu Huan2Xuankang Wang3Zhou Yao4Chunmei Wang5Fang Ren6Hong Wang7Hongyan Qin8Xueyu Hu9Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaDepartment of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi ChinaState Key Laboratory of Cancer Biology, Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Xi'an, 710032 Shaanxi China; Corresponding author.Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032 Shaanxi China; Corresponding author.To explore the relationship between diabetes and intervertebral disc degeneration in mice and the associated underlying mechanism. Four-week-old male Kunming mice were used to model diabetes using a high-fat diet combined with streptozotocin injection. After 6 months, morphological and pathological changes in L4-L6 intervertebral discs were detected by magnetic resonance imaging, micro-CT and histological staining. Immunostaining of CD31, F4/80 and CD16/32 receptors was used to detect vascular invasion and inflammatory infiltration in endplates; the exact changes were then explored by the transmission electron microscopy. The nucleus pulposus of the control and the diabetic group had a clear boundary and regular shape without collapse, while endplate calcification and chondrocyte abnormality in the diabetic group were more obvious. Immunofluorescence confirmed that compared to control, expression levels of CD31 (vascular endothelial marker) and F4/80 (monocyte/macrophage marker) in the diabetic group were significantly increased (P < 0.05), with an elevated number of F4/80 (+)/CD16/32 (+) cells (P < 0.05). The morphology of endplates was observed by transmission electron microscopy, which showed monocytes/macrophage accumulation in the endplate of the diabetic group, accompanied by increased vascular density, collagen fiber distortion and chondrocyte abnormality. In a conclusion, diabetes promotes endplate degeneration with vascular invasion, monocyte/macrophage infiltration and inflammation in mice.http://www.sciencedirect.com/science/article/pii/S2405844023005029DiabetesEndplateVascular invasionMonocyte/macrophage infiltrationInflammation |
| spellingShingle | Huilin Quan Xiaoshuang Zuo Yu Huan Xuankang Wang Zhou Yao Chunmei Wang Fang Ren Hong Wang Hongyan Qin Xueyu Hu A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice Heliyon Diabetes Endplate Vascular invasion Monocyte/macrophage infiltration Inflammation |
| title | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| title_full | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| title_fullStr | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| title_full_unstemmed | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| title_short | A systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| title_sort | systematic morphology study on the effect of high glucose on intervertebral disc endplate degeneration in mice |
| topic | Diabetes Endplate Vascular invasion Monocyte/macrophage infiltration Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023005029 |
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