In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect
Additively manufactured biodegradable zinc (Zn) scaffolds have great potential to repair infected bone defects due to their osteogenic and antibacterial properties. However, the enhancement of antibacterial properties depends on a high concentration of dissolved Zn2+, which in return deteriorates os...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423003459 |
| _version_ | 1797355251610681344 |
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| author | Xiang Jin Dongxu Xie Zhenbao Zhang Aobo Liu Menglin Wang Jiabao Dai Xuan Wang Huanze Deng Yijie Liang Yantao Zhao Peng Wen Yanfeng Li |
| author_facet | Xiang Jin Dongxu Xie Zhenbao Zhang Aobo Liu Menglin Wang Jiabao Dai Xuan Wang Huanze Deng Yijie Liang Yantao Zhao Peng Wen Yanfeng Li |
| author_sort | Xiang Jin |
| collection | DOAJ |
| description | Additively manufactured biodegradable zinc (Zn) scaffolds have great potential to repair infected bone defects due to their osteogenic and antibacterial properties. However, the enhancement of antibacterial properties depends on a high concentration of dissolved Zn2+, which in return deteriorates osteogenic activity. In this study, a vancomycin (Van)-loaded polydopamine (PDA) coating was prepared on pure Zn porous scaffolds to solve the above dilemma. Compared with pure Zn scaffolds according to comprehensive in vitro tests, the PDA coating resulted in a slow degradation and inhibited the excessive release of Zn2+ at the early stage, thus improving cytocompatibility and osteogenic activity. Meanwhile, the addition of Van drug substantially suppressed the attachment and proliferation of S. aureus and E. coli bacterial. Furthermore, in vivo implantation confirmed the simultaneously improved osteogenic and antibacterial functions by using the pure Zn scaffolds with Van-loaded PDA coating. Therefore, it is promising to employ biodegradable Zn porous scaffolds with the proposed drug-loaded coating for the treatment of infected bone defects. |
| first_indexed | 2024-03-08T14:09:33Z |
| format | Article |
| id | doaj.art-f9039364e2294a51b439faa7547194d3 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-08T14:09:33Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-f9039364e2294a51b439faa7547194d32024-01-15T04:23:55ZengElsevierMaterials Today Bio2590-00642024-02-0124100885In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defectXiang Jin0Dongxu Xie1Zhenbao Zhang2Aobo Liu3Menglin Wang4Jiabao Dai5Xuan Wang6Huanze Deng7Yijie Liang8Yantao Zhao9Peng Wen10Yanfeng Li11Postgraduate Training Base, Jinzhou Medical University and The Fourth Medical Centre, Chinese PLA General Hospital, Beijing, 10048, China; Department of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaState Key Laboratory of Tribology in Advanced Equipment, Beijing, 100084, China; Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, ChinaDepartment of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaState Key Laboratory of Tribology in Advanced Equipment, Beijing, 100084, China; Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, ChinaDepartment of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaState Key Laboratory of Tribology in Advanced Equipment, Beijing, 100084, China; Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, ChinaPostgraduate Training Base, Jinzhou Medical University and The Fourth Medical Centre, Chinese PLA General Hospital, Beijing, 10048, China; Department of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaDepartment of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaPostgraduate Training Base, Jinzhou Medical University and The Fourth Medical Centre, Chinese PLA General Hospital, Beijing, 10048, China; Department of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, ChinaDepartment of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, China; Senior Department of Orthopedics, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, China; Beijing Engineering Research Center of Orthopedics Implants, Beijing, 100048, China; Corresponding author. Department of Stomatology, the Fourth Medical Centre, PLA General Hospital, Beijing, 100048, China.State Key Laboratory of Tribology in Advanced Equipment, Beijing, 100084, China; Department of Mechanical Engineering, Tsinghua University, Beijing, 100084, China; Corresponding author. State Key Laboratory of Tribology in Advanced Equipment, Beijing, 100084, China.Department of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, China; Postgraduate Training Base, Jinzhou Medical University and The Fourth Medical Centre, Chinese PLA General Hospital, Beijing, 10048, China; Corresponding author. Department of Stomatology, The Fourth Medical Centre, PLA General Hospital, Beijing, 100048, China.Additively manufactured biodegradable zinc (Zn) scaffolds have great potential to repair infected bone defects due to their osteogenic and antibacterial properties. However, the enhancement of antibacterial properties depends on a high concentration of dissolved Zn2+, which in return deteriorates osteogenic activity. In this study, a vancomycin (Van)-loaded polydopamine (PDA) coating was prepared on pure Zn porous scaffolds to solve the above dilemma. Compared with pure Zn scaffolds according to comprehensive in vitro tests, the PDA coating resulted in a slow degradation and inhibited the excessive release of Zn2+ at the early stage, thus improving cytocompatibility and osteogenic activity. Meanwhile, the addition of Van drug substantially suppressed the attachment and proliferation of S. aureus and E. coli bacterial. Furthermore, in vivo implantation confirmed the simultaneously improved osteogenic and antibacterial functions by using the pure Zn scaffolds with Van-loaded PDA coating. Therefore, it is promising to employ biodegradable Zn porous scaffolds with the proposed drug-loaded coating for the treatment of infected bone defects.http://www.sciencedirect.com/science/article/pii/S2590006423003459Additive manufacturingPure ZnPorous scaffoldBone repairAnti-infection |
| spellingShingle | Xiang Jin Dongxu Xie Zhenbao Zhang Aobo Liu Menglin Wang Jiabao Dai Xuan Wang Huanze Deng Yijie Liang Yantao Zhao Peng Wen Yanfeng Li In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect Materials Today Bio Additive manufacturing Pure Zn Porous scaffold Bone repair Anti-infection |
| title | In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect |
| title_full | In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect |
| title_fullStr | In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect |
| title_full_unstemmed | In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect |
| title_short | In vitro and in vivo studies on biodegradable Zn porous scaffolds with a drug-loaded coating for the treatment of infected bone defect |
| title_sort | in vitro and in vivo studies on biodegradable zn porous scaffolds with a drug loaded coating for the treatment of infected bone defect |
| topic | Additive manufacturing Pure Zn Porous scaffold Bone repair Anti-infection |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423003459 |
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