BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry
Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immu...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Tehran
2018-10-01
|
| Series: | Iranian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | https://ijvm.ut.ac.ir/article_68554_c1882a67da80659d741c532de35a01a7.pdf |
| _version_ | 1818724034556198912 |
|---|---|
| author | Keivan Jamshidi Ozlem Ozmen |
| author_facet | Keivan Jamshidi Ozlem Ozmen |
| author_sort | Keivan Jamshidi |
| collection | DOAJ |
| description | Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. RESULTS: Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of259 sheep (6.35%). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3 % and 11.6 %, respectively. Pneumonias were microscopically classified in sheep as interstitial pneumonia (49.8%), suppurative bronchopneumonia (15.7%), bronchointerstitial pneumonia (11.1 %), and parasitic pneumonia (14.3%). A total of 220 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of BHV1 antigen, were considered. BHV1 antigen was determined to be 8.63 % by the immunohistochemistry (IHC) method. CONCLUSIONS: In conclusion, the presence of viral antigen in lung tissues of sheep may indicate that natural pneumonia may be induced by BHV1 or possibly other species-specific herpesviruses. Moreover, it is suggested that sheep might have a role in the transmission of this virus to cattle. |
| first_indexed | 2024-12-17T21:20:00Z |
| format | Article |
| id | doaj.art-f903f1c184c941829289ef4d3f3bedb8 |
| institution | Directory Open Access Journal |
| issn | 2251-8894 2252-0554 |
| language | English |
| last_indexed | 2024-12-17T21:20:00Z |
| publishDate | 2018-10-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Iranian Journal of Veterinary Medicine |
| spelling | doaj.art-f903f1c184c941829289ef4d3f3bedb82022-12-21T21:32:14ZengUniversity of TehranIranian Journal of Veterinary Medicine2251-88942252-05542018-10-0112431332110.22059/ijvm.2018.233674.100489068554BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using ImmunohistochemistryKeivan Jamshidi0Ozlem Ozmen1Department of Veterinary Pathology, Faculty of Veterinary Medicine, IAU Garmsar Branch, Garmsar, IranDepartment of Veterinary Pathology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, TurkeyBackground: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which were raised in various farms in the Garmsar district and surrounding areas and were brought to the local abattoir for slaughtering between April and September 2016, were examined. RESULTS: Macroscopic pneumonia findings were detected in different lobes particularly in the apical and cardiac lobes of the lungs of259 sheep (6.35%). The rates of mild, moderate and severe consolidations observed in the pneumonic lungs were 59.8%, 26.3 % and 11.6 %, respectively. Pneumonias were microscopically classified in sheep as interstitial pneumonia (49.8%), suppurative bronchopneumonia (15.7%), bronchointerstitial pneumonia (11.1 %), and parasitic pneumonia (14.3%). A total of 220 pneumonic lungs, excluding parasitic pneumonia, examination with immunohistochemistry (IH) in terms of BHV1 antigen, were considered. BHV1 antigen was determined to be 8.63 % by the immunohistochemistry (IHC) method. CONCLUSIONS: In conclusion, the presence of viral antigen in lung tissues of sheep may indicate that natural pneumonia may be induced by BHV1 or possibly other species-specific herpesviruses. Moreover, it is suggested that sheep might have a role in the transmission of this virus to cattle.https://ijvm.ut.ac.ir/article_68554_c1882a67da80659d741c532de35a01a7.pdfbhv1immunohistochemistrylungpneumoniasheep |
| spellingShingle | Keivan Jamshidi Ozlem Ozmen BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry Iranian Journal of Veterinary Medicine bhv1 immunohistochemistry lung pneumonia sheep |
| title | BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry |
| title_full | BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry |
| title_fullStr | BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry |
| title_full_unstemmed | BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry |
| title_short | BHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry |
| title_sort | bhv 1 antigen detection in paraffinized lung sections of pneumonic sheep lung using immunohistochemistry |
| topic | bhv1 immunohistochemistry lung pneumonia sheep |
| url | https://ijvm.ut.ac.ir/article_68554_c1882a67da80659d741c532de35a01a7.pdf |
| work_keys_str_mv | AT keivanjamshidi bhv1antigendetectioninparaffinizedlungsectionsofpneumonicsheeplungusingimmunohistochemistry AT ozlemozmen bhv1antigendetectioninparaffinizedlungsectionsofpneumonicsheeplungusingimmunohistochemistry |