Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae
Ribosome biogenesis is essential for cellular function and involves rRNA synthesis, rRNA processing and modification, and ribosomal protein assembly. Ribosome biogenesis factors and small nucleolar RNA assist these events. Ribosomal maturation takes place in the nucleolus, the nucleoplasm, and the c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-09-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12487 |
| _version_ | 1827990601286549504 |
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| author | Denise Palm Deniz Streit Maike Ruprecht Stefan Simm Christian Scharf Enrico Schleiff |
| author_facet | Denise Palm Deniz Streit Maike Ruprecht Stefan Simm Christian Scharf Enrico Schleiff |
| author_sort | Denise Palm |
| collection | DOAJ |
| description | Ribosome biogenesis is essential for cellular function and involves rRNA synthesis, rRNA processing and modification, and ribosomal protein assembly. Ribosome biogenesis factors and small nucleolar RNA assist these events. Ribosomal maturation takes place in the nucleolus, the nucleoplasm, and the cytosol in a coordinated and controlled manner. For example, some ribosomal proteins are thought to be assembled in the cytoplasm based on the observations in Saccharomyces cerevisiae. Here, we used cellular fractionation to demonstrate that cleavage of the 20S intermediate, the precursor to mature 18S rRNA, does not occur in the nucleoplasm of Arabidopsis thaliana. It most likely occurs in the cytoplasm. Further, we verified the proposed localization of RPS10e, RPS26e, and RPL24a/b in the nucleus and RPP1 in the nucleolus of A. thaliana by ribosome profiling, immunofluorescence, and analysis of the localization of GFP fusion proteins. Our results suggest that the order of events during ribosomal protein assembly in the ribosome biogenesis pathway differs between plants and yeast. |
| first_indexed | 2024-04-10T00:38:02Z |
| format | Article |
| id | doaj.art-f90708f69d344841ac3185b5b89d75dc |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-10T00:38:02Z |
| publishDate | 2018-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-f90708f69d344841ac3185b5b89d75dc2023-03-14T13:05:47ZengWileyFEBS Open Bio2211-54632018-09-01891437144410.1002/2211-5463.12487Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiaeDenise Palm0Deniz Streit1Maike Ruprecht2Stefan Simm3Christian Scharf4Enrico Schleiff5Institute for Molecular Biosciences Goethe University Frankfurt am Main GermanyInstitute for Molecular Biosciences Goethe University Frankfurt am Main GermanyInstitute for Molecular Biosciences Goethe University Frankfurt am Main GermanyInstitute for Molecular Biosciences Goethe University Frankfurt am Main GermanyDepartment of Otorhinolaryngology, Head and Neck Surgery University of Greifswald GermanyInstitute for Molecular Biosciences Goethe University Frankfurt am Main GermanyRibosome biogenesis is essential for cellular function and involves rRNA synthesis, rRNA processing and modification, and ribosomal protein assembly. Ribosome biogenesis factors and small nucleolar RNA assist these events. Ribosomal maturation takes place in the nucleolus, the nucleoplasm, and the cytosol in a coordinated and controlled manner. For example, some ribosomal proteins are thought to be assembled in the cytoplasm based on the observations in Saccharomyces cerevisiae. Here, we used cellular fractionation to demonstrate that cleavage of the 20S intermediate, the precursor to mature 18S rRNA, does not occur in the nucleoplasm of Arabidopsis thaliana. It most likely occurs in the cytoplasm. Further, we verified the proposed localization of RPS10e, RPS26e, and RPL24a/b in the nucleus and RPP1 in the nucleolus of A. thaliana by ribosome profiling, immunofluorescence, and analysis of the localization of GFP fusion proteins. Our results suggest that the order of events during ribosomal protein assembly in the ribosome biogenesis pathway differs between plants and yeast.https://doi.org/10.1002/2211-5463.12487Arabidopsis thalianaeukaryotic systemintracellular protein localizationribosomal proteinsribosome biogenesis |
| spellingShingle | Denise Palm Deniz Streit Maike Ruprecht Stefan Simm Christian Scharf Enrico Schleiff Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae FEBS Open Bio Arabidopsis thaliana eukaryotic system intracellular protein localization ribosomal proteins ribosome biogenesis |
| title | Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae |
| title_full | Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae |
| title_fullStr | Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae |
| title_full_unstemmed | Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae |
| title_short | Late ribosomal protein localization in Arabidopsis thaliana differs to that in Saccharomyces cerevisiae |
| title_sort | late ribosomal protein localization in arabidopsis thaliana differs to that in saccharomyces cerevisiae |
| topic | Arabidopsis thaliana eukaryotic system intracellular protein localization ribosomal proteins ribosome biogenesis |
| url | https://doi.org/10.1002/2211-5463.12487 |
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