Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
Faithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyc...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-10-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009808 |
| _version_ | 1818574202254393344 |
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| author | Daniel Corbi Angelika Amon |
| author_facet | Daniel Corbi Angelika Amon |
| author_sort | Daniel Corbi |
| collection | DOAJ |
| description | Faithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyces cerevisiae mtDNA that is preferentially inherited over wild-type mtDNA (rho+), to uncover the factors governing mtDNA inheritance. We found that some regions of rho+ mtDNA persisted while others were lost after a specific hypersuppressive takeover indicating that hypersuppressive preferential inheritance may partially be due to active destruction of rho+ mtDNA. From a multicopy suppression screen, we found that overexpression of putative mitochondrial RNA exonuclease PET127 reduced biased inheritance of a subset of hypersuppressive genomes. This suppression required PET127 binding to the mitochondrial RNA polymerase RPO41 but not PET127 exonuclease activity. A temperature-sensitive allele of RPO41 improved rho+ mtDNA inheritance over a specific hypersuppressive mtDNA at semi-permissive temperatures revealing a previously unknown role for rho+ transcription in promoting hypersuppressive mtDNA inheritance. |
| first_indexed | 2024-12-15T00:23:26Z |
| format | Article |
| id | doaj.art-f909f2d7178f451eac63b3dc87e1101b |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-15T00:23:26Z |
| publishDate | 2021-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-f909f2d7178f451eac63b3dc87e1101b2022-12-21T22:42:15ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-10-011710e100980810.1371/journal.pgen.1009808Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.Daniel CorbiAngelika AmonFaithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyces cerevisiae mtDNA that is preferentially inherited over wild-type mtDNA (rho+), to uncover the factors governing mtDNA inheritance. We found that some regions of rho+ mtDNA persisted while others were lost after a specific hypersuppressive takeover indicating that hypersuppressive preferential inheritance may partially be due to active destruction of rho+ mtDNA. From a multicopy suppression screen, we found that overexpression of putative mitochondrial RNA exonuclease PET127 reduced biased inheritance of a subset of hypersuppressive genomes. This suppression required PET127 binding to the mitochondrial RNA polymerase RPO41 but not PET127 exonuclease activity. A temperature-sensitive allele of RPO41 improved rho+ mtDNA inheritance over a specific hypersuppressive mtDNA at semi-permissive temperatures revealing a previously unknown role for rho+ transcription in promoting hypersuppressive mtDNA inheritance.https://doi.org/10.1371/journal.pgen.1009808 |
| spellingShingle | Daniel Corbi Angelika Amon Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. PLoS Genetics |
| title | Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. |
| title_full | Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. |
| title_fullStr | Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. |
| title_full_unstemmed | Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. |
| title_short | Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA. |
| title_sort | decreasing mitochondrial rna polymerase activity reverses biased inheritance of hypersuppressive mtdna |
| url | https://doi.org/10.1371/journal.pgen.1009808 |
| work_keys_str_mv | AT danielcorbi decreasingmitochondrialrnapolymeraseactivityreversesbiasedinheritanceofhypersuppressivemtdna AT angelikaamon decreasingmitochondrialrnapolymeraseactivityreversesbiasedinheritanceofhypersuppressivemtdna |