Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook)
Pollen grains produce certain metabolites, which can improve or inhibit germination and tube growth. Metabolomic analysis of germinating and growing Chinese fir pollen has not been reported. Therefore, this study aimed to analyse metabolites changes, content and expression in the germinating pollen...
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MDPI AG
2018-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/23/12/3162 |
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| author | Seif Aldin Dawina Abdallah Fragallah Pei Wang Nuo Li Yu Chen Sizu Lin |
| author_facet | Seif Aldin Dawina Abdallah Fragallah Pei Wang Nuo Li Yu Chen Sizu Lin |
| author_sort | Seif Aldin Dawina Abdallah Fragallah |
| collection | DOAJ |
| description | Pollen grains produce certain metabolites, which can improve or inhibit germination and tube growth. Metabolomic analysis of germinating and growing Chinese fir pollen has not been reported. Therefore, this study aimed to analyse metabolites changes, content and expression in the germinating pollen of Chinese fir. To understand the metabolic differences, two clones from Chinese fir were selected. Metabolomics analyses were performed on three stages (1-, 24- and 48-h) during in vitro pollen germination. The metabolites profiles at different time points were analyzed by using liquid chromatography-mass spectrometry. The results showed that 171 peaks were screened; the corresponding differential metabolites of 121 peaks were classified into nine types of substances. The expression of metabolites showed significant differences across and between clones, and the variation was evident at all germination stages. The expression was obvious at the early stage of germination, which differed clearly from that of the late stage after pollen tube growth. Moreover, the metabolites were mainly enriched in 14 metabolic pathways. Pollen germination and tube growth and metabolites expressions changed per incubation time. Since this work is preliminary, we suggest further investigations to understand the relationship between the differential metabolites and pollen development, and factors affecting pollen germination process. |
| first_indexed | 2024-12-10T09:41:50Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T09:41:50Z |
| publishDate | 2018-11-01 |
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| series | Molecules |
| spelling | doaj.art-f90afd2e4ed5499695c71452137f03a72022-12-22T01:53:58ZengMDPI AGMolecules1420-30492018-11-012312316210.3390/molecules23123162molecules23123162Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook)Seif Aldin Dawina Abdallah Fragallah0Pei Wang1Nuo Li2Yu Chen3Sizu Lin4College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaPollen grains produce certain metabolites, which can improve or inhibit germination and tube growth. Metabolomic analysis of germinating and growing Chinese fir pollen has not been reported. Therefore, this study aimed to analyse metabolites changes, content and expression in the germinating pollen of Chinese fir. To understand the metabolic differences, two clones from Chinese fir were selected. Metabolomics analyses were performed on three stages (1-, 24- and 48-h) during in vitro pollen germination. The metabolites profiles at different time points were analyzed by using liquid chromatography-mass spectrometry. The results showed that 171 peaks were screened; the corresponding differential metabolites of 121 peaks were classified into nine types of substances. The expression of metabolites showed significant differences across and between clones, and the variation was evident at all germination stages. The expression was obvious at the early stage of germination, which differed clearly from that of the late stage after pollen tube growth. Moreover, the metabolites were mainly enriched in 14 metabolic pathways. Pollen germination and tube growth and metabolites expressions changed per incubation time. Since this work is preliminary, we suggest further investigations to understand the relationship between the differential metabolites and pollen development, and factors affecting pollen germination process.https://www.mdpi.com/1420-3049/23/12/3162Chinese firmetabolomicsin-vitro pollen germinationmetabolic differences |
| spellingShingle | Seif Aldin Dawina Abdallah Fragallah Pei Wang Nuo Li Yu Chen Sizu Lin Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) Molecules Chinese fir metabolomics in-vitro pollen germination metabolic differences |
| title | Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) |
| title_full | Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) |
| title_fullStr | Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) |
| title_full_unstemmed | Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) |
| title_short | Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (<i>Cunninghamia lanceolata</i> (Lamb) Hook) |
| title_sort | metabolomic analysis of pollen grains with different germination abilities from two clones of chinese fir i cunninghamia lanceolata i lamb hook |
| topic | Chinese fir metabolomics in-vitro pollen germination metabolic differences |
| url | https://www.mdpi.com/1420-3049/23/12/3162 |
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