| Summary: | A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of <i>Alternaria alternata</i> and <i>Fusarium solani</i> treated with cell-free extracts of <i>P. fluorescens</i>. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of <i>P. fluorescens</i> contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of <i>P. fluorescens</i> and chitosan [50–1.5% (<i>v</i>/<i>v</i>)] had a synergistic effect since they notably inhibited the mycelial growth of <i>A. altenata</i> and <i>F. solani</i>. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents.
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