Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combinati...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6359 |
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| author | Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo |
| author_facet | Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo |
| author_sort | Ariadna Berenice Trejo-Raya |
| collection | DOAJ |
| description | A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of <i>Alternaria alternata</i> and <i>Fusarium solani</i> treated with cell-free extracts of <i>P. fluorescens</i>. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of <i>P. fluorescens</i> contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of <i>P. fluorescens</i> and chitosan [50–1.5% (<i>v</i>/<i>v</i>)] had a synergistic effect since they notably inhibited the mycelial growth of <i>A. altenata</i> and <i>F. solani</i>. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents. |
| first_indexed | 2024-03-10T05:57:21Z |
| format | Article |
| id | doaj.art-f90cb5eb197949918207d2b978e99aa1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:57:21Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f90cb5eb197949918207d2b978e99aa12023-11-22T21:20:06ZengMDPI AGMolecules1420-30492021-10-012621635910.3390/molecules26216359Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and ChitosanAriadna Berenice Trejo-Raya0Víctor Manuel Rodríguez-Romero1Silvia Bautista-Baños2Francisco Roberto Quiroz-Figueroa3Ramón Villanueva-Arce4Enrique Durán-Páramo5Laboratory of Food Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional (IPN), Av. Acueducto s/n, Barrio La Laguna Ticomán, Ciudad de México CP 07340, MexicoLaboratory of Food Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional (IPN), Av. Acueducto s/n, Barrio La Laguna Ticomán, Ciudad de México CP 07340, MexicoCentro de Desarrollo de Productos Bióticos (CeProBi), Department of Postharvest Technology, IPN, Km 8.5 Carretera Yautepec-Jojutla, San Isidro, Yautepec CP 62731, Morelos, MexicoLaboratory of Molecular Plant Breeding, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa (CIIDIR-IPN Unidad Sinaloa), Juan de Dios Batiz Paredes No. 250, San Joaquin, Guasave CP 81101, Sinaloa, MexicoLaboratory of Food Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional (IPN), Av. Acueducto s/n, Barrio La Laguna Ticomán, Ciudad de México CP 07340, MexicoLaboratory of Food Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología (UPIBI), Instituto Politécnico Nacional (IPN), Av. Acueducto s/n, Barrio La Laguna Ticomán, Ciudad de México CP 07340, MexicoA biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of <i>Alternaria alternata</i> and <i>Fusarium solani</i> treated with cell-free extracts of <i>P. fluorescens</i>. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of <i>P. fluorescens</i> contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of <i>P. fluorescens</i> and chitosan [50–1.5% (<i>v</i>/<i>v</i>)] had a synergistic effect since they notably inhibited the mycelial growth of <i>A. altenata</i> and <i>F. solani</i>. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents.https://www.mdpi.com/1420-3049/26/21/6359<i>Alternaria altenata</i><i>Fusarium solani</i>siderophoresantagonistbiofungicide |
| spellingShingle | Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan Molecules <i>Alternaria altenata</i> <i>Fusarium solani</i> siderophores antagonist biofungicide |
| title | Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan |
| title_full | Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan |
| title_fullStr | Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan |
| title_full_unstemmed | Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan |
| title_short | Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan |
| title_sort | effective in vitro control of two phytopathogens of agricultural interest using cell free extracts of i pseudomonas fluorescens i and chitosan |
| topic | <i>Alternaria altenata</i> <i>Fusarium solani</i> siderophores antagonist biofungicide |
| url | https://www.mdpi.com/1420-3049/26/21/6359 |
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