Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica
The benthic bacterial community in Antarctic continental shelf ecosystems are not well-documented. We collected 13 surface sediments from the Ross Sea, a biological hotspot in high-latitude maritime Antarctica undergoing rapid climate change and possible microflora shift, and aimed to study the dive...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02523/full |
| _version_ | 1818150776784027648 |
|---|---|
| author | An-Zhang Li Xi-Bin Han Ming-Xia Zhang Yang Zhou Meng Chen Qing Yao Hong-Hui Zhu |
| author_facet | An-Zhang Li Xi-Bin Han Ming-Xia Zhang Yang Zhou Meng Chen Qing Yao Hong-Hui Zhu |
| author_sort | An-Zhang Li |
| collection | DOAJ |
| description | The benthic bacterial community in Antarctic continental shelf ecosystems are not well-documented. We collected 13 surface sediments from the Ross Sea, a biological hotspot in high-latitude maritime Antarctica undergoing rapid climate change and possible microflora shift, and aimed to study the diversity, structure and assembly mechanism of benthic bacterial community using both culture-dependent and -independent approaches. High-throughput sequencing of 16S rRNA gene amplicons revealed 370 OTUs distributed in 21 phyla and 284 genera. The bacterial community was dominated by Bacteroidetes, Gamma- and Alphaproteobacteria, and constituted by a compact, conserved and positively-correlated group of anaerobes and other competitive aerobic chemoheterotrophs. Null-model test based on βNTI and RCBray indicated that stochastic processes, including dispersal limitation and undominated fractions, were the main forces driving community assembly. On the other hand, environmental factors, mainly temperature, organic matter and chlorophyll, were significantly correlated with bacterial richness, diversity and community structure. Moreover, metabolic and physiological features of the prokaryotic taxa were mapped to evaluate the adaptive mechanisms and functional composition of the benthic bacterial community. Our study is helpful to understand the structural and functional aspects, as well as the ecological and biogeochemical role of the benthic bacterial community in the Ross Sea. |
| first_indexed | 2024-12-11T13:28:19Z |
| format | Article |
| id | doaj.art-f90e637a197541c9999e83472ec0f7d6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T13:28:19Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f90e637a197541c9999e83472ec0f7d62022-12-22T01:05:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02523481042Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, AntarcticaAn-Zhang Li0Xi-Bin Han1Ming-Xia Zhang2Yang Zhou3Meng Chen4Qing Yao5Hong-Hui Zhu6State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaKey Laboratory of Submarine Geosciences, Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangdong Engineering Research Center for Grass Science, Guangdong Engineering Center for Litchi, College of Horticulture, South China Agricultural University, Guangzhou, ChinaState Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, ChinaThe benthic bacterial community in Antarctic continental shelf ecosystems are not well-documented. We collected 13 surface sediments from the Ross Sea, a biological hotspot in high-latitude maritime Antarctica undergoing rapid climate change and possible microflora shift, and aimed to study the diversity, structure and assembly mechanism of benthic bacterial community using both culture-dependent and -independent approaches. High-throughput sequencing of 16S rRNA gene amplicons revealed 370 OTUs distributed in 21 phyla and 284 genera. The bacterial community was dominated by Bacteroidetes, Gamma- and Alphaproteobacteria, and constituted by a compact, conserved and positively-correlated group of anaerobes and other competitive aerobic chemoheterotrophs. Null-model test based on βNTI and RCBray indicated that stochastic processes, including dispersal limitation and undominated fractions, were the main forces driving community assembly. On the other hand, environmental factors, mainly temperature, organic matter and chlorophyll, were significantly correlated with bacterial richness, diversity and community structure. Moreover, metabolic and physiological features of the prokaryotic taxa were mapped to evaluate the adaptive mechanisms and functional composition of the benthic bacterial community. Our study is helpful to understand the structural and functional aspects, as well as the ecological and biogeochemical role of the benthic bacterial community in the Ross Sea.https://www.frontiersin.org/article/10.3389/fmicb.2019.02523/fullRoss Seabacterial diversitycommunity structuremicrofloracommunity assemblyhigh-throughput sequencing |
| spellingShingle | An-Zhang Li Xi-Bin Han Ming-Xia Zhang Yang Zhou Meng Chen Qing Yao Hong-Hui Zhu Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica Frontiers in Microbiology Ross Sea bacterial diversity community structure microflora community assembly high-throughput sequencing |
| title | Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica |
| title_full | Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica |
| title_fullStr | Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica |
| title_full_unstemmed | Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica |
| title_short | Culture-Dependent and -Independent Analyses Reveal the Diversity, Structure, and Assembly Mechanism of Benthic Bacterial Community in the Ross Sea, Antarctica |
| title_sort | culture dependent and independent analyses reveal the diversity structure and assembly mechanism of benthic bacterial community in the ross sea antarctica |
| topic | Ross Sea bacterial diversity community structure microflora community assembly high-throughput sequencing |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02523/full |
| work_keys_str_mv | AT anzhangli culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT xibinhan culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT mingxiazhang culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT yangzhou culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT mengchen culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT qingyao culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica AT honghuizhu culturedependentandindependentanalysesrevealthediversitystructureandassemblymechanismofbenthicbacterialcommunityintherossseaantarctica |