Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolera...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13677 |
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| author | Kiril Mishev Petre I. Dobrev Jozef Lacek Roberta Filepová Bistra Yuperlieva-Mateeva Anelia Kostadinova Tsveta Hristeva |
| author_facet | Kiril Mishev Petre I. Dobrev Jozef Lacek Roberta Filepová Bistra Yuperlieva-Mateeva Anelia Kostadinova Tsveta Hristeva |
| author_sort | Kiril Mishev |
| collection | DOAJ |
| description | Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolerance. However, parasitic plants have adopted the capacity to sense the same signaling molecules and to trigger their own seed germination in the immediate vicinity of host roots. The contribution of AM fungi and parasitic plants to the regulation of phytohormone levels in host plant roots and root exudates remains largely obscure. Here, we studied the hormonome in the model system comprising tobacco as a host plant, <i>Phelipanche</i> spp. as a holoparasitic plant, and the AM fungus <i>Rhizophagus irregularis</i>. Co-cultivation of tobacco with broomrape and AM fungi alone or in combination led to characteristic changes in the levels of endogenous and exuded abscisic acid, indole-3-acetic acid, cytokinins, salicylic acid, and orobanchol-type strigolactones. The hormonal content in exudates of broomrape-infested mycorrhizal roots resembled that in exudates of infested non-mycorrhizal roots and differed from that observed in exudates of non-infested mycorrhizal roots. Moreover, we observed a significant reduction in AM colonization of infested tobacco plants, pointing to a dominant role of the holoparasite within the tripartite system. |
| first_indexed | 2024-03-10T03:55:11Z |
| format | Article |
| id | doaj.art-f90f6a1efbdd463180455a8fa452a850 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:55:11Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f90f6a1efbdd463180455a8fa452a8502023-11-23T08:49:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241367710.3390/ijms222413677Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal FungiKiril Mishev0Petre I. Dobrev1Jozef Lacek2Roberta Filepová3Bistra Yuperlieva-Mateeva4Anelia Kostadinova5Tsveta Hristeva6Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, 165 02 Praha, Czech RepublicLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, 165 02 Praha, Czech RepublicLaboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, 165 02 Praha, Czech RepublicInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaTobacco and Tobacco Products Institute, Agricultural Academy, 4108 Plovdiv, BulgariaBelowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolerance. However, parasitic plants have adopted the capacity to sense the same signaling molecules and to trigger their own seed germination in the immediate vicinity of host roots. The contribution of AM fungi and parasitic plants to the regulation of phytohormone levels in host plant roots and root exudates remains largely obscure. Here, we studied the hormonome in the model system comprising tobacco as a host plant, <i>Phelipanche</i> spp. as a holoparasitic plant, and the AM fungus <i>Rhizophagus irregularis</i>. Co-cultivation of tobacco with broomrape and AM fungi alone or in combination led to characteristic changes in the levels of endogenous and exuded abscisic acid, indole-3-acetic acid, cytokinins, salicylic acid, and orobanchol-type strigolactones. The hormonal content in exudates of broomrape-infested mycorrhizal roots resembled that in exudates of infested non-mycorrhizal roots and differed from that observed in exudates of non-infested mycorrhizal roots. Moreover, we observed a significant reduction in AM colonization of infested tobacco plants, pointing to a dominant role of the holoparasite within the tripartite system.https://www.mdpi.com/1422-0067/22/24/13677parasitic plantsmycorrhizal fungiplant hormonesroot exudatesrhizospheresmall-molecule communication |
| spellingShingle | Kiril Mishev Petre I. Dobrev Jozef Lacek Roberta Filepová Bistra Yuperlieva-Mateeva Anelia Kostadinova Tsveta Hristeva Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi International Journal of Molecular Sciences parasitic plants mycorrhizal fungi plant hormones root exudates rhizosphere small-molecule communication |
| title | Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi |
| title_full | Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi |
| title_fullStr | Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi |
| title_full_unstemmed | Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi |
| title_short | Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi |
| title_sort | hormonomic changes driving the negative impact of broomrape on plant host interactions with arbuscular mycorrhizal fungi |
| topic | parasitic plants mycorrhizal fungi plant hormones root exudates rhizosphere small-molecule communication |
| url | https://www.mdpi.com/1422-0067/22/24/13677 |
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