Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress
Potato is a drought-sensitive crop whose global sustainable production is threatened by alterations in water availability. Whilst ancestral Solanum tuberosum Andigenum landraces retain wild drought tolerance mechanisms, their molecular bases remain poorly understood. In this study, an aeroponic grow...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1003907/full |
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| author | Olga Patricia Ponce Yerisf Torres Yerisf Torres Ankush Prashar Robin Buell Roberto Lozano Roberto Lozano Gisella Orjeda Lindsey Compton |
| author_facet | Olga Patricia Ponce Yerisf Torres Yerisf Torres Ankush Prashar Robin Buell Roberto Lozano Roberto Lozano Gisella Orjeda Lindsey Compton |
| author_sort | Olga Patricia Ponce |
| collection | DOAJ |
| description | Potato is a drought-sensitive crop whose global sustainable production is threatened by alterations in water availability. Whilst ancestral Solanum tuberosum Andigenum landraces retain wild drought tolerance mechanisms, their molecular bases remain poorly understood. In this study, an aeroponic growth system was established to investigate stress responses in leaf and root of two Andigenum varieties with contrasting drought tolerance. Comparative transcriptome analysis revealed widespread differences in the response of the two varieties at early and late time points of exposure to drought stress and in the recovery after rewatering. Major differences in the response of the two varieties occurred at the early time point, suggesting the speed of response is crucial. In the leaves and roots of the tolerant variety, we observed rapid upregulation of ABA-related genes, which did not occur until later in the susceptible variety and indicated not only more effective ABA synthesis and mobilization, but more effective feedback regulation to limit detrimental effects of too much ABA. Roots of both varieties showed differential expression of genes involved in cell wall reinforcement and remodeling to maintain cell wall strength, hydration and growth under drought stress, including genes involved in lignification and wall expansion, though the response was stronger in the tolerant variety. Such changes in leaf and root may help to limit water losses in the tolerant variety, while limiting the reduction in photosynthetic rate. These findings provide insights into molecular bases of drought tolerance mechanisms and pave the way for their reintroduction into modern cultivars with improved resistance to drought stress and yield stability under drought conditions. |
| first_indexed | 2024-12-10T06:17:53Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T06:17:53Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f9100dbbbb97407ba7a237e05fc0f5be2022-12-22T01:59:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10039071003907Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stressOlga Patricia Ponce0Yerisf Torres1Yerisf Torres2Ankush Prashar3Robin Buell4Roberto Lozano5Roberto Lozano6Gisella Orjeda7Lindsey Compton8School of Biosciences, University of Birmingham, Birmingham, United KingdomDepartment of Plant Science, Wageningen University, Wageningen, NetherlandsUnidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, Lima, PeruSchool of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United KingdomDepartment of Crop & Soil Sciences, Institute for Plant Breeding, Genetics & Genomics, Center for Applied Genetic Technology, University of Georgia, Athens, GA, United StatesUnidad de genómica, Laboratorios de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, Lima, PeruDigital Science and Technology Department, Joyn Bio LLC, Boston, MA, United StatesFaculty of Biological Sciences, Universidad Nacional Mayor de San Marcos, Lima, PeruSchool of Biosciences, University of Birmingham, Birmingham, United KingdomPotato is a drought-sensitive crop whose global sustainable production is threatened by alterations in water availability. Whilst ancestral Solanum tuberosum Andigenum landraces retain wild drought tolerance mechanisms, their molecular bases remain poorly understood. In this study, an aeroponic growth system was established to investigate stress responses in leaf and root of two Andigenum varieties with contrasting drought tolerance. Comparative transcriptome analysis revealed widespread differences in the response of the two varieties at early and late time points of exposure to drought stress and in the recovery after rewatering. Major differences in the response of the two varieties occurred at the early time point, suggesting the speed of response is crucial. In the leaves and roots of the tolerant variety, we observed rapid upregulation of ABA-related genes, which did not occur until later in the susceptible variety and indicated not only more effective ABA synthesis and mobilization, but more effective feedback regulation to limit detrimental effects of too much ABA. Roots of both varieties showed differential expression of genes involved in cell wall reinforcement and remodeling to maintain cell wall strength, hydration and growth under drought stress, including genes involved in lignification and wall expansion, though the response was stronger in the tolerant variety. Such changes in leaf and root may help to limit water losses in the tolerant variety, while limiting the reduction in photosynthetic rate. These findings provide insights into molecular bases of drought tolerance mechanisms and pave the way for their reintroduction into modern cultivars with improved resistance to drought stress and yield stability under drought conditions.https://www.frontiersin.org/articles/10.3389/fpls.2022.1003907/fulldroughtpotatoabiotic stressrehydrationtranscriptome |
| spellingShingle | Olga Patricia Ponce Yerisf Torres Yerisf Torres Ankush Prashar Robin Buell Roberto Lozano Roberto Lozano Gisella Orjeda Lindsey Compton Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress Frontiers in Plant Science drought potato abiotic stress rehydration transcriptome |
| title | Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress |
| title_full | Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress |
| title_fullStr | Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress |
| title_full_unstemmed | Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress |
| title_short | Transcriptome profiling shows a rapid variety-specific response in two Andigenum potato varieties under drought stress |
| title_sort | transcriptome profiling shows a rapid variety specific response in two andigenum potato varieties under drought stress |
| topic | drought potato abiotic stress rehydration transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1003907/full |
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