Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran
Entamoeba histolytica and Entamoeba dispar can not be differentiated with each other in microscopic examination of stool but isoenzyme electrophoresis is the gold standard technique. Isolated Entamoeba histolytica and Entamoeba dispar from stool samples of patients who referred to clinical laborator...
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| Format: | Article |
| Language: | English |
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Bushehr University of Medical Sciences
2005-02-01
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| Series: | Iranian South Medical Journal |
| Subjects: | |
| Online Access: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-88&slc_lang=en&sid=1 |
| _version_ | 1828975672522964992 |
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| author | Nematollah Sahebani Lame Akhlaghi Hoormozd Urmazdi Gholamreza Hatam Mohammad Hossain Motazedian |
| author_facet | Nematollah Sahebani Lame Akhlaghi Hoormozd Urmazdi Gholamreza Hatam Mohammad Hossain Motazedian |
| author_sort | Nematollah Sahebani |
| collection | DOAJ |
| description | Entamoeba histolytica and Entamoeba dispar can not be differentiated with each other in microscopic examination of stool but isoenzyme electrophoresis is the gold standard technique. Isolated Entamoeba histolytica and Entamoeba dispar from stool samples of patients who referred to clinical laboratories in the south parts of Iran were collected. These samples were cultivated in Robinson's medium and after mass cultivation and separation of their enzymes isoenzyme electrophoresis for four enzymes (Malic Enzyme, Glucose Phosphate Isomerase, Phospho Gluco Mutase, and Hexokinase) was done. A total of 23 positive samples were collected, six samples (16%) were diagnosed as Entamoeba histolytica and 17 samples (74%) as Entamoeba dispar. Zymodemes of Entamoeba histolytica were II and XIV and, zymodemes of Entamoeba dispar were I, XVI, XVII and XVIII. In conclusion, isoenzyme electrophoresis showed that the most reported samples as Entamoeba histolytica were Entamoeba dispar. |
| first_indexed | 2024-12-14T14:25:06Z |
| format | Article |
| id | doaj.art-f9141e7182564825a0dcf2094c5572e7 |
| institution | Directory Open Access Journal |
| issn | 1735-4374 1735-6954 |
| language | English |
| last_indexed | 2024-12-14T14:25:06Z |
| publishDate | 2005-02-01 |
| publisher | Bushehr University of Medical Sciences |
| record_format | Article |
| series | Iranian South Medical Journal |
| spelling | doaj.art-f9141e7182564825a0dcf2094c5572e72022-12-21T22:57:59ZengBushehr University of Medical SciencesIranian South Medical Journal1735-43741735-69542005-02-0172107112Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of IranNematollah Sahebani0Lame Akhlaghi1Hoormozd Urmazdi2Gholamreza Hatam3Mohammad Hossain Motazedian4 Entamoeba histolytica and Entamoeba dispar can not be differentiated with each other in microscopic examination of stool but isoenzyme electrophoresis is the gold standard technique. Isolated Entamoeba histolytica and Entamoeba dispar from stool samples of patients who referred to clinical laboratories in the south parts of Iran were collected. These samples were cultivated in Robinson's medium and after mass cultivation and separation of their enzymes isoenzyme electrophoresis for four enzymes (Malic Enzyme, Glucose Phosphate Isomerase, Phospho Gluco Mutase, and Hexokinase) was done. A total of 23 positive samples were collected, six samples (16%) were diagnosed as Entamoeba histolytica and 17 samples (74%) as Entamoeba dispar. Zymodemes of Entamoeba histolytica were II and XIV and, zymodemes of Entamoeba dispar were I, XVI, XVII and XVIII. In conclusion, isoenzyme electrophoresis showed that the most reported samples as Entamoeba histolytica were Entamoeba dispar.http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-88&slc_lang=en&sid=1Entamoeba histolytica Entamoeba dispar isoenzyme zymodeme |
| spellingShingle | Nematollah Sahebani Lame Akhlaghi Hoormozd Urmazdi Gholamreza Hatam Mohammad Hossain Motazedian Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran Iranian South Medical Journal Entamoeba histolytica Entamoeba dispar isoenzyme zymodeme |
| title | Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran |
| title_full | Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran |
| title_fullStr | Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran |
| title_full_unstemmed | Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran |
| title_short | Examination and separation of zymodemes of Entamoeba histolytica and Entamoeba dispar in the south of Iran |
| title_sort | examination and separation of zymodemes of entamoeba histolytica and entamoeba dispar in the south of iran |
| topic | Entamoeba histolytica Entamoeba dispar isoenzyme zymodeme |
| url | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-88&slc_lang=en&sid=1 |
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