Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans
Coniothyrium minitans is a sclerotial parasite, which has been investigated for commercial control of crop diseases caused by Sclerotinia sclerotiorum. Previously, we obtained a T-DNA insertional mutant, ZS-1TN24363, which did not produce melanin during conidiation. To understand the function of mel...
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| Format: | Article |
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Frontiers Media S.A.
2018-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02658/full |
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| author | Chenwei Luo Chenwei Luo Huizhang Zhao Huizhang Zhao Xiaoxiang Yang Xiaoxiang Yang Cuicui Qiang Cuicui Qiang Jiasen Cheng Jiasen Cheng Jiatao Xie Jiatao Xie Tao Chen Daohong Jiang Daohong Jiang Yanping Fu |
| author_facet | Chenwei Luo Chenwei Luo Huizhang Zhao Huizhang Zhao Xiaoxiang Yang Xiaoxiang Yang Cuicui Qiang Cuicui Qiang Jiasen Cheng Jiasen Cheng Jiatao Xie Jiatao Xie Tao Chen Daohong Jiang Daohong Jiang Yanping Fu |
| author_sort | Chenwei Luo |
| collection | DOAJ |
| description | Coniothyrium minitans is a sclerotial parasite, which has been investigated for commercial control of crop diseases caused by Sclerotinia sclerotiorum. Previously, we obtained a T-DNA insertional mutant, ZS-1TN24363, which did not produce melanin during conidiation. To understand the function of melanin in C. minitans, we cloned the gene that was disrupted by the T-DNA insertion, and found that this gene, called CmMR1, encoded a putative protein of 1,011 amino acids, which is a homolog of the transcription factor MR. Full-length CmMR1 contains 3,167 bp, with three exons and two introns. To confirm that the disrupted gene is responsible for the melanin-deficiency of the mutant, CmMR1 was disrupted and three targeted knockout mutants were obtained. Biological assays showed that the phenotype of the targeted knockout mutants was similar to that of the T-DNA insertional mutant. Furthermore, gene complementation confirmed that CmMR1 is responsible for the mutant phenotype. CmMR1 disruption did not affect hyphal growth, conidiation, and parasitization of C. minitans, however, the ROS accumulation increased and tolerance to UV light decreased significantly in the mutants. Our result may enhance the understanding of melanin in the ecology of C. minitans on molecular level. |
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| language | English |
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| publishDate | 2018-11-01 |
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| spelling | doaj.art-f91725231999403b9d0b6c55e729003f2022-12-21T18:21:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-11-01910.3389/fmicb.2018.02658415260Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitansChenwei Luo0Chenwei Luo1Huizhang Zhao2Huizhang Zhao3Xiaoxiang Yang4Xiaoxiang Yang5Cuicui Qiang6Cuicui Qiang7Jiasen Cheng8Jiasen Cheng9Jiatao Xie10Jiatao Xie11Tao Chen12Daohong Jiang13Daohong Jiang14Yanping Fu15State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaConiothyrium minitans is a sclerotial parasite, which has been investigated for commercial control of crop diseases caused by Sclerotinia sclerotiorum. Previously, we obtained a T-DNA insertional mutant, ZS-1TN24363, which did not produce melanin during conidiation. To understand the function of melanin in C. minitans, we cloned the gene that was disrupted by the T-DNA insertion, and found that this gene, called CmMR1, encoded a putative protein of 1,011 amino acids, which is a homolog of the transcription factor MR. Full-length CmMR1 contains 3,167 bp, with three exons and two introns. To confirm that the disrupted gene is responsible for the melanin-deficiency of the mutant, CmMR1 was disrupted and three targeted knockout mutants were obtained. Biological assays showed that the phenotype of the targeted knockout mutants was similar to that of the T-DNA insertional mutant. Furthermore, gene complementation confirmed that CmMR1 is responsible for the mutant phenotype. CmMR1 disruption did not affect hyphal growth, conidiation, and parasitization of C. minitans, however, the ROS accumulation increased and tolerance to UV light decreased significantly in the mutants. Our result may enhance the understanding of melanin in the ecology of C. minitans on molecular level.https://www.frontiersin.org/article/10.3389/fmicb.2018.02658/fullConiothyrium minitansmelanintranscription factor MRROSUV-tolerance |
| spellingShingle | Chenwei Luo Chenwei Luo Huizhang Zhao Huizhang Zhao Xiaoxiang Yang Xiaoxiang Yang Cuicui Qiang Cuicui Qiang Jiasen Cheng Jiasen Cheng Jiatao Xie Jiatao Xie Tao Chen Daohong Jiang Daohong Jiang Yanping Fu Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans Frontiers in Microbiology Coniothyrium minitans melanin transcription factor MR ROS UV-tolerance |
| title | Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans |
| title_full | Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans |
| title_fullStr | Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans |
| title_full_unstemmed | Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans |
| title_short | Functional Analysis of the Melanin-Associated Gene CmMR1 in Coniothyrium minitans |
| title_sort | functional analysis of the melanin associated gene cmmr1 in coniothyrium minitans |
| topic | Coniothyrium minitans melanin transcription factor MR ROS UV-tolerance |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02658/full |
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