The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment
A study of alginate-chitosan membrane synthesize was done. The membrane was prepared by mixing alginate hydrosol and chitosan hydrosol at mass ratio of 1:1 and pH of 5.28 approximately. Then it was applied for matrix immobilization of urease and bromothymol blue (BTB) by entrapment technique. The ph...
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| Format: | Article |
| Language: | English |
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Jenderal Soedirman University
2020-03-01
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| Series: | Molekul |
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| Online Access: | https://ojs.jmolekul.com/ojs/index.php/jm/article/view/527 |
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| author | Dhony Hermanto Mudasir Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli |
| author_facet | Dhony Hermanto Mudasir Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli |
| author_sort | Dhony Hermanto |
| collection | DOAJ |
| description | A study of alginate-chitosan membrane synthesize was done. The membrane was prepared by mixing alginate hydrosol and chitosan hydrosol at mass ratio of 1:1 and pH of 5.28 approximately. Then it was applied for matrix immobilization of urease and bromothymol blue (BTB) by entrapment technique. The physical, chemical, thermal properties of alginate–chitosan membrane and their impact on immobilized urease activity were investigated. The polymer products were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). It’s showed that alginate-chitosan membrane was formed by electrostatic interaction. The obtained membran has better mechanical properties than original alginat and chitosan membranes. The immobilization urease into alginate-chitosan membrane retained the catalytic activity of the enzyme, as confirmed by color change of BTB indicator after membrane was immersed in substrate solution (urea). Therefore, alginate-chitosan membrane has good characteristics as matrix of enzyme immobilization. |
| first_indexed | 2024-04-12T07:15:36Z |
| format | Article |
| id | doaj.art-f918e24949ee4dd299f4d8ccae35de94 |
| institution | Directory Open Access Journal |
| issn | 1907-9761 2503-0310 |
| language | English |
| last_indexed | 2024-04-12T07:15:36Z |
| publishDate | 2020-03-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Molekul |
| spelling | doaj.art-f918e24949ee4dd299f4d8ccae35de942022-12-22T03:42:30ZengJenderal Soedirman UniversityMolekul1907-97612503-03102020-03-01151404710.20884/1.jm.2020.15.1.527291The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease EntrapmentDhony Hermanto0Mudasir Mudasir1Dwi Siswanta2Bambang Kuswandi3Nurul Ismillayli4Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram, Mataram 83125, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University Gadjah Mada, Yogyakarta 55281, IndonesiaChemo and Biosensor Group, Faculty of Pharmacy, University of Jember, Jember East Java 68121, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, University of Mataram, Mataram 83125, IndonesiaA study of alginate-chitosan membrane synthesize was done. The membrane was prepared by mixing alginate hydrosol and chitosan hydrosol at mass ratio of 1:1 and pH of 5.28 approximately. Then it was applied for matrix immobilization of urease and bromothymol blue (BTB) by entrapment technique. The physical, chemical, thermal properties of alginate–chitosan membrane and their impact on immobilized urease activity were investigated. The polymer products were characterized by Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). It’s showed that alginate-chitosan membrane was formed by electrostatic interaction. The obtained membran has better mechanical properties than original alginat and chitosan membranes. The immobilization urease into alginate-chitosan membrane retained the catalytic activity of the enzyme, as confirmed by color change of BTB indicator after membrane was immersed in substrate solution (urea). Therefore, alginate-chitosan membrane has good characteristics as matrix of enzyme immobilization.https://ojs.jmolekul.com/ojs/index.php/jm/article/view/527alginate–chitosan, membrane, preparation, characterization, application |
| spellingShingle | Dhony Hermanto Mudasir Mudasir Dwi Siswanta Bambang Kuswandi Nurul Ismillayli The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment Molekul alginate–chitosan, membrane, preparation, characterization, application |
| title | The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment |
| title_full | The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment |
| title_fullStr | The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment |
| title_full_unstemmed | The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment |
| title_short | The Preparation and Characterization of Alginate–Chitosan Membranes as Solid Support for BTB and Urease Entrapment |
| title_sort | preparation and characterization of alginate chitosan membranes as solid support for btb and urease entrapment |
| topic | alginate–chitosan, membrane, preparation, characterization, application |
| url | https://ojs.jmolekul.com/ojs/index.php/jm/article/view/527 |
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