Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging
Summary: Ferroptosis is a non-apoptotic iron-dependent cell death. Here we present a protocol for stratifying ferroptosis sensitivity in cells and mouse tissues. This protocol uses photochemical activation of lipid peroxidation (PALP) coupled with fluorescent imaging to assess the relative sensitivi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000697 |
| _version_ | 1818307861038497792 |
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| author | Fengxiang Wang Nathchar Naowarojna Yilong Zou |
| author_facet | Fengxiang Wang Nathchar Naowarojna Yilong Zou |
| author_sort | Fengxiang Wang |
| collection | DOAJ |
| description | Summary: Ferroptosis is a non-apoptotic iron-dependent cell death. Here we present a protocol for stratifying ferroptosis sensitivity in cells and mouse tissues. This protocol uses photochemical activation of lipid peroxidation (PALP) coupled with fluorescent imaging to assess the relative sensitivity to ferroptosis. Using commercial reagents and common equipment, PALP is readily accessible to most laboratories. One remaining challenge is the inability to multiplex this technique in analyzing multiple tissues or regions simultaneously. This protocol may have applications in developing ferroptosis-targeted therapies.For complete details on the use and execution of this protocol, please refer to Wang et al. (2021). |
| first_indexed | 2024-12-13T07:05:06Z |
| format | Article |
| id | doaj.art-f91a96c6b8824c92becb39ed53b41688 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T07:05:06Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-f91a96c6b8824c92becb39ed53b416882022-12-21T23:55:51ZengElsevierSTAR Protocols2666-16672022-06-0132101189Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imagingFengxiang Wang0Nathchar Naowarojna1Yilong Zou2College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058 China; Westlake Four-Dimensional Dynamic Metabolomics (Meta4D) Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024 China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024 China; Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024 China; Corresponding authorWestlake Four-Dimensional Dynamic Metabolomics (Meta4D) Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024 China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024 China; Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024 ChinaWestlake Four-Dimensional Dynamic Metabolomics (Meta4D) Lab, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang 310024 China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024 China; Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024 China; Corresponding authorSummary: Ferroptosis is a non-apoptotic iron-dependent cell death. Here we present a protocol for stratifying ferroptosis sensitivity in cells and mouse tissues. This protocol uses photochemical activation of lipid peroxidation (PALP) coupled with fluorescent imaging to assess the relative sensitivity to ferroptosis. Using commercial reagents and common equipment, PALP is readily accessible to most laboratories. One remaining challenge is the inability to multiplex this technique in analyzing multiple tissues or regions simultaneously. This protocol may have applications in developing ferroptosis-targeted therapies.For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722000697Cell BiologyCell MembraneCancerMetabolismMicroscopy |
| spellingShingle | Fengxiang Wang Nathchar Naowarojna Yilong Zou Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging STAR Protocols Cell Biology Cell Membrane Cancer Metabolism Microscopy |
| title | Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| title_full | Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| title_fullStr | Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| title_full_unstemmed | Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| title_short | Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| title_sort | stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging |
| topic | Cell Biology Cell Membrane Cancer Metabolism Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722000697 |
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