Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis
Although increasing evidence is emerging on the contribution of chemical elements in periodontal health, no studies have concomitantly evaluated the ionic profile in gingival crevicular fluid (GCF) and saliva in relation to the underlying periodontal status. Our hypothesis is that these biofluids ha...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/3/687 |
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| author | Federica Romano Giovanni Iaderosa Matteo Corana Stefano Perotto Giacomo Baima Federica Di Scipio Giuliana Abbadessa Giulia Maria Mariani Mario Aimetti Giovanni Nicolao Berta |
| author_facet | Federica Romano Giovanni Iaderosa Matteo Corana Stefano Perotto Giacomo Baima Federica Di Scipio Giuliana Abbadessa Giulia Maria Mariani Mario Aimetti Giovanni Nicolao Berta |
| author_sort | Federica Romano |
| collection | DOAJ |
| description | Although increasing evidence is emerging on the contribution of chemical elements in periodontal health, no studies have concomitantly evaluated the ionic profile in gingival crevicular fluid (GCF) and saliva in relation to the underlying periodontal status. Our hypothesis is that these biofluids have distinctive ionic content. Therefore, the aim of this cross-sectional study was to analyze the elemental composition of GCF and saliva in order to explore which biological matrix and which combination of elements could discriminate between periodontitis and periodontal health. Twelve ions were analyzed in GCF and unstimulated saliva from 54 subjects (18 periodontally healthy, 18 untreated severe periodontitis and 18 treated severe periodontitis) using inductively coupled plasma–mass spectrometry (ICP-MS) and inductively coupled plasma–optical emission spectroscopy (ICP-OES). These analytical techniques were able to determine levels of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg), while the other elements were below the detection threshold. Na and K ions were detected at elevated concentration in untreated periodontitis compared with treated periodontitis and healthy periodontium. Ca was increased in untreated periodontitis, but the difference was not significant. In saliva, only Na was significantly associated with periodontitis. The combination of Na and K in GCF enabled the correct assignment of a subject to the periodontitis or healthy group. Based on these preliminary results, GCF demonstrated higher clustering potential than saliva. |
| first_indexed | 2024-03-09T20:05:23Z |
| format | Article |
| id | doaj.art-f91eb0f8591040d09959eb58e7ad5967 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:05:23Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-f91eb0f8591040d09959eb58e7ad59672023-11-24T00:33:41ZengMDPI AGBiomedicines2227-90592022-03-0110368710.3390/biomedicines10030687Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe PeriodontitisFederica Romano0Giovanni Iaderosa1Matteo Corana2Stefano Perotto3Giacomo Baima4Federica Di Scipio5Giuliana Abbadessa6Giulia Maria Mariani7Mario Aimetti8Giovanni Nicolao Berta9Department of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, ItalyDepartment of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Surgical Sciences, C.I.R. Dental School, Section of Periodontology, University of Turin, 10126 Turin, ItalyDepartment of Clinical and Biological Sciences, University of Turin, 10043 Orbassano, ItalyAlthough increasing evidence is emerging on the contribution of chemical elements in periodontal health, no studies have concomitantly evaluated the ionic profile in gingival crevicular fluid (GCF) and saliva in relation to the underlying periodontal status. Our hypothesis is that these biofluids have distinctive ionic content. Therefore, the aim of this cross-sectional study was to analyze the elemental composition of GCF and saliva in order to explore which biological matrix and which combination of elements could discriminate between periodontitis and periodontal health. Twelve ions were analyzed in GCF and unstimulated saliva from 54 subjects (18 periodontally healthy, 18 untreated severe periodontitis and 18 treated severe periodontitis) using inductively coupled plasma–mass spectrometry (ICP-MS) and inductively coupled plasma–optical emission spectroscopy (ICP-OES). These analytical techniques were able to determine levels of sodium (Na), potassium (K), calcium (Ca) and magnesium (Mg), while the other elements were below the detection threshold. Na and K ions were detected at elevated concentration in untreated periodontitis compared with treated periodontitis and healthy periodontium. Ca was increased in untreated periodontitis, but the difference was not significant. In saliva, only Na was significantly associated with periodontitis. The combination of Na and K in GCF enabled the correct assignment of a subject to the periodontitis or healthy group. Based on these preliminary results, GCF demonstrated higher clustering potential than saliva.https://www.mdpi.com/2227-9059/10/3/687gingival crevicular fluidICP-MSICP-OESionomicsmetallomicsperiodontitis |
| spellingShingle | Federica Romano Giovanni Iaderosa Matteo Corana Stefano Perotto Giacomo Baima Federica Di Scipio Giuliana Abbadessa Giulia Maria Mariani Mario Aimetti Giovanni Nicolao Berta Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis Biomedicines gingival crevicular fluid ICP-MS ICP-OES ionomics metallomics periodontitis |
| title | Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis |
| title_full | Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis |
| title_fullStr | Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis |
| title_full_unstemmed | Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis |
| title_short | Comparing Ionic Profile of Gingival Crevicular Fluid and Saliva as Distinctive Signature of Severe Periodontitis |
| title_sort | comparing ionic profile of gingival crevicular fluid and saliva as distinctive signature of severe periodontitis |
| topic | gingival crevicular fluid ICP-MS ICP-OES ionomics metallomics periodontitis |
| url | https://www.mdpi.com/2227-9059/10/3/687 |
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